Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa

Wild-type Neurospora crassa grown in minimal medium was exposed to -difluormethyl ornithine (DFMO), a specific inhibitor of ornithine-decarboxylase (ODC-ase) activity. Protein-synthesis rates impaired by DFMO were restored by the addition of spermidine. The pattern on SDS-acrylamide gels displayed t...

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Detalles Bibliográficos
Publicado:	1990
Materias:	gene activation Neurospora crassa ODC-ase inhibition polyamine deficiency ornithine decarboxylase polyamine article fungus gene expression neurospora crassa nonhuman priority journal Eflornithine Enzyme Inhibitors Gene Expression Regulation, Fungal Neurospora Ornithine Decarboxylase Polyamines Spermidine Fungi
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v14_n1_p69_Grotewold http://hdl.handle.net/20.500.12110/paper_03091651_v14_n1_p69_Grotewold
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03091651_v14_n1_p69_Grotewold
record_format	dspace
spelling	paper:paper_03091651_v14_n1_p69_Grotewold2023-06-08T15:31:48Z Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa gene activation Neurospora crassa ODC-ase inhibition polyamine deficiency ornithine decarboxylase polyamine article fungus gene expression neurospora crassa nonhuman priority journal Eflornithine Enzyme Inhibitors Gene Expression Regulation, Fungal Neurospora Neurospora crassa Ornithine Decarboxylase Polyamines Spermidine Fungi Neurospora crassa Wild-type Neurospora crassa grown in minimal medium was exposed to -difluormethyl ornithine (DFMO), a specific inhibitor of ornithine-decarboxylase (ODC-ase) activity. Protein-synthesis rates impaired by DFMO were restored by the addition of spermidine. The pattern on SDS-acrylamide gels displayed three newly synthesized polypeptides, p27, p31 and p99 after DFMO action in the absence of exogenous polyamine. The ODC-ase mutant (spe-1) grown in spermidine-supplemented medium did not show an induced polypeptide pattern. The lack of ODC-ase activity promotes the expression of p27- and p31-coding genes in both strains but transcription of p31 gene is shut-off after spermidine addition. Both transcripts are also accumulated after exposure to low cycloheximide doses or nutrient starvation. Another cycloheximide-inducible gene coding for p70 is also expressed under DFMO-treatment. © 1990. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v14_n1_p69_Grotewold http://hdl.handle.net/20.500.12110/paper_03091651_v14_n1_p69_Grotewold
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	gene activation Neurospora crassa ODC-ase inhibition polyamine deficiency ornithine decarboxylase polyamine article fungus gene expression neurospora crassa nonhuman priority journal Eflornithine Enzyme Inhibitors Gene Expression Regulation, Fungal Neurospora Neurospora crassa Ornithine Decarboxylase Polyamines Spermidine Fungi Neurospora crassa
spellingShingle	gene activation Neurospora crassa ODC-ase inhibition polyamine deficiency ornithine decarboxylase polyamine article fungus gene expression neurospora crassa nonhuman priority journal Eflornithine Enzyme Inhibitors Gene Expression Regulation, Fungal Neurospora Neurospora crassa Ornithine Decarboxylase Polyamines Spermidine Fungi Neurospora crassa Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa
topic_facet	gene activation Neurospora crassa ODC-ase inhibition polyamine deficiency ornithine decarboxylase polyamine article fungus gene expression neurospora crassa nonhuman priority journal Eflornithine Enzyme Inhibitors Gene Expression Regulation, Fungal Neurospora Neurospora crassa Ornithine Decarboxylase Polyamines Spermidine Fungi Neurospora crassa
description	Wild-type Neurospora crassa grown in minimal medium was exposed to -difluormethyl ornithine (DFMO), a specific inhibitor of ornithine-decarboxylase (ODC-ase) activity. Protein-synthesis rates impaired by DFMO were restored by the addition of spermidine. The pattern on SDS-acrylamide gels displayed three newly synthesized polypeptides, p27, p31 and p99 after DFMO action in the absence of exogenous polyamine. The ODC-ase mutant (spe-1) grown in spermidine-supplemented medium did not show an induced polypeptide pattern. The lack of ODC-ase activity promotes the expression of p27- and p31-coding genes in both strains but transcription of p31 gene is shut-off after spermidine addition. Both transcripts are also accumulated after exposure to low cycloheximide doses or nutrient starvation. Another cycloheximide-inducible gene coding for p70 is also expressed under DFMO-treatment. © 1990.
title	Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa
title_short	Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa
title_full	Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa
title_fullStr	Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa
title_full_unstemmed	Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa
title_sort	genes responsive to the alteration of polyamine biosynthesis in neurospora crassa
publishDate	1990
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v14_n1_p69_Grotewold http://hdl.handle.net/20.500.12110/paper_03091651_v14_n1_p69_Grotewold
_version_	1768544820853211136

Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa

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