cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans

Intracellular levels of cAMP and specific activities of adenylate cyclase, cAMP phosphodiesterase and cAMP-dependent protein kinase were measured during filamentation in the dimorphic fungus Candida albicans. Enzymatic assays were performed in permeabilized cells under conditions prevented endogenou...

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Detalles Bibliográficos
Autores principales:	Egidy, Giorgia Anna Rosa, Paveto, María Cristina, Galvagno, Miguel Angel
Publicado:	1990
Materias:	adenylate cyclase cyclic amp cyclic amp dependent protein kinase cyclic amp phosphodiesterase article candida albicans differentiation fungus morphology nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Candida albicans Cyclic AMP Fungi
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v14_n1_p59_Egidy http://hdl.handle.net/20.500.12110/paper_03091651_v14_n1_p59_Egidy
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03091651_v14_n1_p59_Egidy
record_format	dspace
spelling	paper:paper_03091651_v14_n1_p59_Egidy2023-06-08T15:31:47Z cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans Egidy, Giorgia Anna Rosa Paveto, María Cristina Galvagno, Miguel Angel adenylate cyclase cyclic amp cyclic amp dependent protein kinase cyclic amp phosphodiesterase article candida albicans differentiation fungus morphology nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Candida albicans Cyclic AMP Candida albicans Fungi Intracellular levels of cAMP and specific activities of adenylate cyclase, cAMP phosphodiesterase and cAMP-dependent protein kinase were measured during filamentation in the dimorphic fungus Candida albicans. Enzymatic assays were performed in permeabilized cells under conditions prevented endogenous proteolysis. The variations observed in cAMP levels were mainly accounted for by variations in the specific activities of adenylate cyclase and cAMP phosphodiesterase at different stages during germ tube formation. cAMP-dependent protein kinase, measured with kemptide as exogenous substrate, was developmental regulated. Some properties of the enzymatic activities from cell-free extracts are described. © 1990. Fil:Egidy, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paveto, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Galvagno, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v14_n1_p59_Egidy http://hdl.handle.net/20.500.12110/paper_03091651_v14_n1_p59_Egidy
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	adenylate cyclase cyclic amp cyclic amp dependent protein kinase cyclic amp phosphodiesterase article candida albicans differentiation fungus morphology nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Candida albicans Cyclic AMP Candida albicans Fungi
spellingShingle	adenylate cyclase cyclic amp cyclic amp dependent protein kinase cyclic amp phosphodiesterase article candida albicans differentiation fungus morphology nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Candida albicans Cyclic AMP Candida albicans Fungi Egidy, Giorgia Anna Rosa Paveto, María Cristina Galvagno, Miguel Angel cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans
topic_facet	adenylate cyclase cyclic amp cyclic amp dependent protein kinase cyclic amp phosphodiesterase article candida albicans differentiation fungus morphology nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Candida albicans Cyclic AMP Candida albicans Fungi
description	Intracellular levels of cAMP and specific activities of adenylate cyclase, cAMP phosphodiesterase and cAMP-dependent protein kinase were measured during filamentation in the dimorphic fungus Candida albicans. Enzymatic assays were performed in permeabilized cells under conditions prevented endogenous proteolysis. The variations observed in cAMP levels were mainly accounted for by variations in the specific activities of adenylate cyclase and cAMP phosphodiesterase at different stages during germ tube formation. cAMP-dependent protein kinase, measured with kemptide as exogenous substrate, was developmental regulated. Some properties of the enzymatic activities from cell-free extracts are described. © 1990.
author	Egidy, Giorgia Anna Rosa Paveto, María Cristina Galvagno, Miguel Angel
author_facet	Egidy, Giorgia Anna Rosa Paveto, María Cristina Galvagno, Miguel Angel
author_sort	Egidy, Giorgia Anna Rosa
title	cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans
title_short	cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans
title_full	cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans
title_fullStr	cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans
title_full_unstemmed	cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans
title_sort	camp levels and in situ measurement of camp related enzymes during yeast-to-hyphae transition in candida albicans
publishDate	1990
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v14_n1_p59_Egidy http://hdl.handle.net/20.500.12110/paper_03091651_v14_n1_p59_Egidy
work_keys_str_mv	AT egidygiorgiaannarosa camplevelsandinsitumeasurementofcamprelatedenzymesduringyeasttohyphaetransitionincandidaalbicans AT pavetomariacristina camplevelsandinsitumeasurementofcamprelatedenzymesduringyeasttohyphaetransitionincandidaalbicans AT galvagnomiguelangel camplevelsandinsitumeasurementofcamprelatedenzymesduringyeasttohyphaetransitionincandidaalbicans
_version_	1768544866634039296

cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans

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