N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants

Drosophila melanogaster Meigen mutants for N-β-alanyldopamine (NBAD) metabolism have altered levels of NBAD, dopamine and other neurotransmitters. The ebony1 mutant strain has very low levels of NBAD and higher levels of dopamine, whereas the opposite situation is observed in the tan1 mutant. Dopami...

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Detalles Bibliográficos
Autores principales:	Quesada Allué, Luis Alberto, Pérez, Martín Mariano
Publicado:	2015
Materias:	Dopamine ebony Locomotor activity N-β-alanyldopamine Sleep tan Wild-type enzyme activity fly locomotion metabolism mutation sleep Drosophila melanogaster
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03076962_v40_n2_p166_Rossi http://hdl.handle.net/20.500.12110/paper_03076962_v40_n2_p166_Rossi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03076962_v40_n2_p166_Rossi
record_format	dspace
spelling	paper:paper_03076962_v40_n2_p166_Rossi2023-06-08T15:31:32Z N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants Quesada Allué, Luis Alberto Pérez, Martín Mariano Dopamine ebony Locomotor activity N-β-alanyldopamine Sleep tan Wild-type enzyme activity fly locomotion metabolism mutation sleep Drosophila melanogaster Drosophila melanogaster Meigen mutants for N-β-alanyldopamine (NBAD) metabolism have altered levels of NBAD, dopamine and other neurotransmitters. The ebony1 mutant strain has very low levels of NBAD and higher levels of dopamine, whereas the opposite situation is observed in the tan1 mutant. Dopamine is implicated in the control of movement, memory and arousal, as well as in the regulation of sleep and wakefulness in D. melanogaster. N-β-alanyldopamine, which is best known as a cuticle cross-linking agent, is also present in nervous tissue and has been proposed to promote locomotor activity in this fly. The daily locomotor activity and the sleep patterns of ebony1 and tan1 mutants are analyzed, and are compared with wild-type flies. The tan1 mutant shows reduced locomotor activity, whereas ebony1 shows higher levels of activity than wild-type flies, suggesting that NBAD does not promote locomotor activity. Both mutants spend less time asleep than wild-type flies during night-time; ebony shows more consolidated activity during night-time and increased sleep latency, whereas tan is unable to consolidate locomotor activity and sleep in either phase of the day. The daily level of NBAD-synthase activity is measured in vitro using wild-type and tan1 protein extracts, and the lowest NBAD synthesis is observed at the time of higher locomotor activity. The abnormalities in several parameters of the waking/sleep cycle indicate some dysfunction in the processes that regulates these behaviours in both mutants. © 2015 The Royal Entomological Society. Fil:Quesada-Allué, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03076962_v40_n2_p166_Rossi http://hdl.handle.net/20.500.12110/paper_03076962_v40_n2_p166_Rossi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Dopamine ebony Locomotor activity N-β-alanyldopamine Sleep tan Wild-type enzyme activity fly locomotion metabolism mutation sleep Drosophila melanogaster
spellingShingle	Dopamine ebony Locomotor activity N-β-alanyldopamine Sleep tan Wild-type enzyme activity fly locomotion metabolism mutation sleep Drosophila melanogaster Quesada Allué, Luis Alberto Pérez, Martín Mariano N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
topic_facet	Dopamine ebony Locomotor activity N-β-alanyldopamine Sleep tan Wild-type enzyme activity fly locomotion metabolism mutation sleep Drosophila melanogaster
description	Drosophila melanogaster Meigen mutants for N-β-alanyldopamine (NBAD) metabolism have altered levels of NBAD, dopamine and other neurotransmitters. The ebony1 mutant strain has very low levels of NBAD and higher levels of dopamine, whereas the opposite situation is observed in the tan1 mutant. Dopamine is implicated in the control of movement, memory and arousal, as well as in the regulation of sleep and wakefulness in D. melanogaster. N-β-alanyldopamine, which is best known as a cuticle cross-linking agent, is also present in nervous tissue and has been proposed to promote locomotor activity in this fly. The daily locomotor activity and the sleep patterns of ebony1 and tan1 mutants are analyzed, and are compared with wild-type flies. The tan1 mutant shows reduced locomotor activity, whereas ebony1 shows higher levels of activity than wild-type flies, suggesting that NBAD does not promote locomotor activity. Both mutants spend less time asleep than wild-type flies during night-time; ebony shows more consolidated activity during night-time and increased sleep latency, whereas tan is unable to consolidate locomotor activity and sleep in either phase of the day. The daily level of NBAD-synthase activity is measured in vitro using wild-type and tan1 protein extracts, and the lowest NBAD synthesis is observed at the time of higher locomotor activity. The abnormalities in several parameters of the waking/sleep cycle indicate some dysfunction in the processes that regulates these behaviours in both mutants. © 2015 The Royal Entomological Society.
author	Quesada Allué, Luis Alberto Pérez, Martín Mariano
author_facet	Quesada Allué, Luis Alberto Pérez, Martín Mariano
author_sort	Quesada Allué, Luis Alberto
title	N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_short	N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_full	N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_fullStr	N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_full_unstemmed	N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants
title_sort	n-β-alanyldopamine metabolism, locomotor activity and sleep in drosophila melanogaster ebony and tan mutants
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03076962_v40_n2_p166_Rossi http://hdl.handle.net/20.500.12110/paper_03076962_v40_n2_p166_Rossi
work_keys_str_mv	AT quesadaallueluisalberto nbalanyldopaminemetabolismlocomotoractivityandsleepindrosophilamelanogasterebonyandtanmutants AT perezmartinmariano nbalanyldopaminemetabolismlocomotoractivityandsleepindrosophilamelanogasterebonyandtanmutants
_version_	1768544274385731584

N-β-alanyldopamine metabolism, locomotor activity and sleep in Drosophila melanogaster ebony and tan mutants

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