Postnatal behavioral and inflammatory alterations in female pups prenatally exposed to valproic acid
In Autism Spectrum Disorders (ASD), a bias to a higher incidence in boys than in girls has been reported. With the aim to identify biological mechanisms acting in female animals that could underlie this bias, we used an extensively validated mouse model of ASD: the prenatal exposure to valproic acid...
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paper:paper_03064530_v72_n_p11_Kazlauskas2023-06-08T15:31:21Z Postnatal behavioral and inflammatory alterations in female pups prenatally exposed to valproic acid Kazlauskas, Nadia Lucchina, Luciana Depino, Amaicha Mara Astrocyte Autism spectrum disorder HDAC Hypothalamus-pituitary-adrenal response Microglia Purkinje cells histone H3 valproic acid GABAergic receptor affecting agent valproic acid acoustic reflex adult animal experiment animal model animal tissue Article astrocyte auditory stimulation body weight cell density controlled study developmental stage female grip strength histone acetylation hypothalamus hypophysis adrenal system male microglia mouse nerve cell differentiation nervous system inflammation nonhuman prenatal drug exposure priority journal Purkinje cell sex difference startle reflex stereotypy animal animal behavior brain cell count cytology maternal behavior metabolism physiology pregnancy prenatal exposure Animals Astrocytes Behavior, Animal Brain Cell Count Female GABA Agents Maternal Behavior Mice Microglia Pregnancy Prenatal Exposure Delayed Effects Reflex, Startle Valproic Acid In Autism Spectrum Disorders (ASD), a bias to a higher incidence in boys than in girls has been reported. With the aim to identify biological mechanisms acting in female animals that could underlie this bias, we used an extensively validated mouse model of ASD: the prenatal exposure to valproic acid (VPA). We found postnatal behavioral alterations in female VPA pups: a longer latency in righting reflex at postnatal day (P) 3, and a delay in the acquisition of the acoustic startle response. We also analyzed the density of glial cells in the prefrontal cortex, hippocampus and cerebellum, in VPA and control animals. Female VPA pups showed alterations in the density of astrocytes and microglial cells between P21 and P42, with specific dynamics in each brain region. We also found a decrease in histone 3 acetylation in the cerebellum of female VPA pups at P14, suggesting that the changes in glial cell density could be due to alterations in the epigenetic developmental program. Finally, no differences in maternal behavior were found. Our results show that female VPA pups exhibit behavioral and inflammatory alterations postnatally, although they have been reported to have normal levels of sociability in adulthood. With our work, we contribute to the understanding of biological mechanisms underlying different effects of VPA on male and female rodents, and we hope to help elucidate whether there are factors increasing susceptibility to ASD in boys and/or resilience in girls. © 2016 Elsevier Ltd Fil:Kazlauskas, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lucchina, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Depino, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064530_v72_n_p11_Kazlauskas http://hdl.handle.net/20.500.12110/paper_03064530_v72_n_p11_Kazlauskas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Astrocyte Autism spectrum disorder HDAC Hypothalamus-pituitary-adrenal response Microglia Purkinje cells histone H3 valproic acid GABAergic receptor affecting agent valproic acid acoustic reflex adult animal experiment animal model animal tissue Article astrocyte auditory stimulation body weight cell density controlled study developmental stage female grip strength histone acetylation hypothalamus hypophysis adrenal system male microglia mouse nerve cell differentiation nervous system inflammation nonhuman prenatal drug exposure priority journal Purkinje cell sex difference startle reflex stereotypy animal animal behavior brain cell count cytology maternal behavior metabolism physiology pregnancy prenatal exposure Animals Astrocytes Behavior, Animal Brain Cell Count Female GABA Agents Maternal Behavior Mice Microglia Pregnancy Prenatal Exposure Delayed Effects Reflex, Startle Valproic Acid |
| spellingShingle |
Astrocyte Autism spectrum disorder HDAC Hypothalamus-pituitary-adrenal response Microglia Purkinje cells histone H3 valproic acid GABAergic receptor affecting agent valproic acid acoustic reflex adult animal experiment animal model animal tissue Article astrocyte auditory stimulation body weight cell density controlled study developmental stage female grip strength histone acetylation hypothalamus hypophysis adrenal system male microglia mouse nerve cell differentiation nervous system inflammation nonhuman prenatal drug exposure priority journal Purkinje cell sex difference startle reflex stereotypy animal animal behavior brain cell count cytology maternal behavior metabolism physiology pregnancy prenatal exposure Animals Astrocytes Behavior, Animal Brain Cell Count Female GABA Agents Maternal Behavior Mice Microglia Pregnancy Prenatal Exposure Delayed Effects Reflex, Startle Valproic Acid Kazlauskas, Nadia Lucchina, Luciana Depino, Amaicha Mara Postnatal behavioral and inflammatory alterations in female pups prenatally exposed to valproic acid |
| topic_facet |
Astrocyte Autism spectrum disorder HDAC Hypothalamus-pituitary-adrenal response Microglia Purkinje cells histone H3 valproic acid GABAergic receptor affecting agent valproic acid acoustic reflex adult animal experiment animal model animal tissue Article astrocyte auditory stimulation body weight cell density controlled study developmental stage female grip strength histone acetylation hypothalamus hypophysis adrenal system male microglia mouse nerve cell differentiation nervous system inflammation nonhuman prenatal drug exposure priority journal Purkinje cell sex difference startle reflex stereotypy animal animal behavior brain cell count cytology maternal behavior metabolism physiology pregnancy prenatal exposure Animals Astrocytes Behavior, Animal Brain Cell Count Female GABA Agents Maternal Behavior Mice Microglia Pregnancy Prenatal Exposure Delayed Effects Reflex, Startle Valproic Acid |
| description |
In Autism Spectrum Disorders (ASD), a bias to a higher incidence in boys than in girls has been reported. With the aim to identify biological mechanisms acting in female animals that could underlie this bias, we used an extensively validated mouse model of ASD: the prenatal exposure to valproic acid (VPA). We found postnatal behavioral alterations in female VPA pups: a longer latency in righting reflex at postnatal day (P) 3, and a delay in the acquisition of the acoustic startle response. We also analyzed the density of glial cells in the prefrontal cortex, hippocampus and cerebellum, in VPA and control animals. Female VPA pups showed alterations in the density of astrocytes and microglial cells between P21 and P42, with specific dynamics in each brain region. We also found a decrease in histone 3 acetylation in the cerebellum of female VPA pups at P14, suggesting that the changes in glial cell density could be due to alterations in the epigenetic developmental program. Finally, no differences in maternal behavior were found. Our results show that female VPA pups exhibit behavioral and inflammatory alterations postnatally, although they have been reported to have normal levels of sociability in adulthood. With our work, we contribute to the understanding of biological mechanisms underlying different effects of VPA on male and female rodents, and we hope to help elucidate whether there are factors increasing susceptibility to ASD in boys and/or resilience in girls. © 2016 Elsevier Ltd |
| author |
Kazlauskas, Nadia Lucchina, Luciana Depino, Amaicha Mara |
| author_facet |
Kazlauskas, Nadia Lucchina, Luciana Depino, Amaicha Mara |
| author_sort |
Kazlauskas, Nadia |
| title |
Postnatal behavioral and inflammatory alterations in female pups prenatally exposed to valproic acid |
| title_short |
Postnatal behavioral and inflammatory alterations in female pups prenatally exposed to valproic acid |
| title_full |
Postnatal behavioral and inflammatory alterations in female pups prenatally exposed to valproic acid |
| title_fullStr |
Postnatal behavioral and inflammatory alterations in female pups prenatally exposed to valproic acid |
| title_full_unstemmed |
Postnatal behavioral and inflammatory alterations in female pups prenatally exposed to valproic acid |
| title_sort |
postnatal behavioral and inflammatory alterations in female pups prenatally exposed to valproic acid |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064530_v72_n_p11_Kazlauskas http://hdl.handle.net/20.500.12110/paper_03064530_v72_n_p11_Kazlauskas |
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AT kazlauskasnadia postnatalbehavioralandinflammatoryalterationsinfemalepupsprenatallyexposedtovalproicacid AT lucchinaluciana postnatalbehavioralandinflammatoryalterationsinfemalepupsprenatallyexposedtovalproicacid AT depinoamaichamara postnatalbehavioralandinflammatoryalterationsinfemalepupsprenatallyexposedtovalproicacid |
| _version_ |
1768545692064677888 |