Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice

Since the discovery that long-term memory is dependent on protein synthesis, several transcription factors have been found to participate in the transcriptional activity needed for its consolidation. Among them, NF-kappa B is a constitutive transcription factor whose nuclear activity has proven to b...

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Autores principales:	Salles, Angeles, Romano, Arturo Gabriel, Freudenthal, Ramiro Angel María
Publicado:	2015
Materias:	I kappa B alpha Memory consolidation NF-kappa B Synapse immunoglobulin enhancer binding protein Rela protein, mouse transcription factor RelA animal experiment animal tissue Article avoidance behavior controlled study enzyme activity hippocampus long term memory male memory consolidation mouse nerve cell plasticity nonhuman priority journal protein localization synaptic membrane synaptoplasm animal cell nucleus dendrite electric shock fluorescent antibody technique foot metabolism outbred strain physiology synapse synaptosome Western blotting Animals Animals, Outbred Strains Avoidance Learning Blotting, Western Cell Nucleus Dendrites Electroshock Fluorescent Antibody Technique Foot Hippocampus Male Memory Consolidation Mice Synapses Synaptosomes Transcription Factor RelA
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v291_n_p70_Salles http://hdl.handle.net/20.500.12110/paper_03064522_v291_n_p70_Salles
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03064522_v291_n_p70_Salles
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spelling	paper:paper_03064522_v291_n_p70_Salles2023-06-08T15:31:19Z Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice Salles, Angeles Romano, Arturo Gabriel Freudenthal, Ramiro Angel María I kappa B alpha Memory consolidation NF-kappa B Synapse immunoglobulin enhancer binding protein immunoglobulin enhancer binding protein Rela protein, mouse transcription factor RelA animal experiment animal tissue Article avoidance behavior controlled study enzyme activity hippocampus long term memory male memory consolidation mouse nerve cell plasticity nonhuman priority journal protein localization synaptic membrane synaptoplasm animal avoidance behavior cell nucleus dendrite electric shock fluorescent antibody technique foot hippocampus memory consolidation metabolism outbred strain physiology synapse synaptosome Western blotting Animals Animals, Outbred Strains Avoidance Learning Blotting, Western Cell Nucleus Dendrites Electroshock Fluorescent Antibody Technique Foot Hippocampus Male Memory Consolidation Mice NF-kappa B Synapses Synaptosomes Transcription Factor RelA Since the discovery that long-term memory is dependent on protein synthesis, several transcription factors have been found to participate in the transcriptional activity needed for its consolidation. Among them, NF-kappa B is a constitutive transcription factor whose nuclear activity has proven to be necessary for the consolidation of inhibitory avoidance in mice. This transcription factor has a wide distribution in the nervous system, with a well-reported presence in dendrites and synaptic terminals. Here we report changes in synaptosomal NF-kappa B localization and activity, during long-term memory consolidation. Activity comparison of synaptosomal and nuclear NF-kappa B, indicates different dynamics for both localizations. In this study we identify two pools of synaptosomal NF-kappa B, one obtained with the synaptoplasm (free fraction) and the second bound to the synaptosomal membranes. During the early steps of consolidation the first pool is activated, as the membrane associated transcription factor fraction increases and concomitantly the free fraction decreases. These results suggest that the activation of synaptic NF-kappa B and its translocation to membranes are part of the consolidation of long-term memory in mice. © 2015 IBRO. Fil:Salles, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romano, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Freudenthal, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v291_n_p70_Salles http://hdl.handle.net/20.500.12110/paper_03064522_v291_n_p70_Salles
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	I kappa B alpha Memory consolidation NF-kappa B Synapse immunoglobulin enhancer binding protein immunoglobulin enhancer binding protein Rela protein, mouse transcription factor RelA animal experiment animal tissue Article avoidance behavior controlled study enzyme activity hippocampus long term memory male memory consolidation mouse nerve cell plasticity nonhuman priority journal protein localization synaptic membrane synaptoplasm animal avoidance behavior cell nucleus dendrite electric shock fluorescent antibody technique foot hippocampus memory consolidation metabolism outbred strain physiology synapse synaptosome Western blotting Animals Animals, Outbred Strains Avoidance Learning Blotting, Western Cell Nucleus Dendrites Electroshock Fluorescent Antibody Technique Foot Hippocampus Male Memory Consolidation Mice NF-kappa B Synapses Synaptosomes Transcription Factor RelA
spellingShingle	I kappa B alpha Memory consolidation NF-kappa B Synapse immunoglobulin enhancer binding protein immunoglobulin enhancer binding protein Rela protein, mouse transcription factor RelA animal experiment animal tissue Article avoidance behavior controlled study enzyme activity hippocampus long term memory male memory consolidation mouse nerve cell plasticity nonhuman priority journal protein localization synaptic membrane synaptoplasm animal avoidance behavior cell nucleus dendrite electric shock fluorescent antibody technique foot hippocampus memory consolidation metabolism outbred strain physiology synapse synaptosome Western blotting Animals Animals, Outbred Strains Avoidance Learning Blotting, Western Cell Nucleus Dendrites Electroshock Fluorescent Antibody Technique Foot Hippocampus Male Memory Consolidation Mice NF-kappa B Synapses Synaptosomes Transcription Factor RelA Salles, Angeles Romano, Arturo Gabriel Freudenthal, Ramiro Angel María Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice
topic_facet	I kappa B alpha Memory consolidation NF-kappa B Synapse immunoglobulin enhancer binding protein immunoglobulin enhancer binding protein Rela protein, mouse transcription factor RelA animal experiment animal tissue Article avoidance behavior controlled study enzyme activity hippocampus long term memory male memory consolidation mouse nerve cell plasticity nonhuman priority journal protein localization synaptic membrane synaptoplasm animal avoidance behavior cell nucleus dendrite electric shock fluorescent antibody technique foot hippocampus memory consolidation metabolism outbred strain physiology synapse synaptosome Western blotting Animals Animals, Outbred Strains Avoidance Learning Blotting, Western Cell Nucleus Dendrites Electroshock Fluorescent Antibody Technique Foot Hippocampus Male Memory Consolidation Mice NF-kappa B Synapses Synaptosomes Transcription Factor RelA
description	Since the discovery that long-term memory is dependent on protein synthesis, several transcription factors have been found to participate in the transcriptional activity needed for its consolidation. Among them, NF-kappa B is a constitutive transcription factor whose nuclear activity has proven to be necessary for the consolidation of inhibitory avoidance in mice. This transcription factor has a wide distribution in the nervous system, with a well-reported presence in dendrites and synaptic terminals. Here we report changes in synaptosomal NF-kappa B localization and activity, during long-term memory consolidation. Activity comparison of synaptosomal and nuclear NF-kappa B, indicates different dynamics for both localizations. In this study we identify two pools of synaptosomal NF-kappa B, one obtained with the synaptoplasm (free fraction) and the second bound to the synaptosomal membranes. During the early steps of consolidation the first pool is activated, as the membrane associated transcription factor fraction increases and concomitantly the free fraction decreases. These results suggest that the activation of synaptic NF-kappa B and its translocation to membranes are part of the consolidation of long-term memory in mice. © 2015 IBRO.
author	Salles, Angeles Romano, Arturo Gabriel Freudenthal, Ramiro Angel María
author_facet	Salles, Angeles Romano, Arturo Gabriel Freudenthal, Ramiro Angel María
author_sort	Salles, Angeles
title	Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice
title_short	Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice
title_full	Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice
title_fullStr	Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice
title_full_unstemmed	Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice
title_sort	hippocampal dynamics of synaptic nf-kappa b during inhibitory avoidance long-term memory consolidation in mice
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v291_n_p70_Salles http://hdl.handle.net/20.500.12110/paper_03064522_v291_n_p70_Salles
work_keys_str_mv	AT sallesangeles hippocampaldynamicsofsynapticnfkappabduringinhibitoryavoidancelongtermmemoryconsolidationinmice AT romanoarturogabriel hippocampaldynamicsofsynapticnfkappabduringinhibitoryavoidancelongtermmemoryconsolidationinmice AT freudenthalramiroangelmaria hippocampaldynamicsofsynapticnfkappabduringinhibitoryavoidancelongtermmemoryconsolidationinmice
_version_	1768544730528874496

Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice

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