Progesterone effects on neuronal brain-derived neurotrophic factor and glial cells during progression of Wobbler mouse neurodegeneration
Previous results have shown a depletion of brain-derived neurotrophic factor (BDNF) mRNA in the degenerating motoneurons from clinically afflicted Wobbler mice, whereas progesterone treatment reverts this depletion. We now compared progesterone regulation of BDNF in motoneurons and oligodendrocytes...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v201_n_p267_Meyer http://hdl.handle.net/20.500.12110/paper_03064522_v201_n_p267_Meyer |
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paper:paper_03064522_v201_n_p267_Meyer2023-06-08T15:31:17Z Progesterone effects on neuronal brain-derived neurotrophic factor and glial cells during progression of Wobbler mouse neurodegeneration Brain-derived neurotrophic factor Motoneuron degeneration Neuroprotection Oligodendrocytes Progesterone Wobbler mouse brain derived neurotrophic factor progesterone animal experiment animal model animal tissue article astrocyte autoradiography controlled study disease course enzyme linked immunosorbent assay female gene expression glia cell gray matter immunohistochemistry in situ hybridization male motoneuron mouse nerve degeneration nerve fiber transport nonhuman oligodendroglia priority journal protein expression spinal cord ventral horn upregulation Age Factors Animals Brain-Derived Neurotrophic Factor Disease Models, Animal Disease Progression Enzyme-Linked Immunosorbent Assay Gene Expression Regulation Mice Mice, Neurologic Mutants Motor Neuron Disease Mutation Neuroglia Neurons Progesterone RNA, Messenger Vesicular Transport Proteins Previous results have shown a depletion of brain-derived neurotrophic factor (BDNF) mRNA in the degenerating motoneurons from clinically afflicted Wobbler mice, whereas progesterone treatment reverts this depletion. We now compared progesterone regulation of BDNF in motoneurons and oligodendrocytes of Wobbler mice at the progressive (EP, 1-3 months), symptomatic (SYM, 5-8 months old), and late stages (LS, 12-13 months). As controls we used NFR/NFR mice. Controls and Wobbler mice of different ages remained untreated or received a 20 mg progesterone pellet during 18 days. BDNF mRNA was determined in the ventral, intermediolateral, and dorsal gray matter by film autoradiography and in motoneurons using in situ hybridization. A depletion of BDNF mRNA already occurred at the EP stage of Wobblers, but progesterone was inactive at this period. In contrast, progesterone upregulated the low levels of BDNF mRNA in SYM Wobblers in the three gray matter regions analyzed. Progesterone also increased BDNF mRNA in LS Wobblers, according to grain counting procedures. BDNF protein analyzed by enzyme-linked immunosorbent assay (ELISA) in ventral horns or immunostaining of motoneurons was normal in steroid-naive SYM Wobblers. BDNF protein was decreased by progesterone, suggesting increased anterograde transport and/or release of neuronal BDNF. Wobbler mice also showed depletion of CC1-immunopositive oligodendrocytes, whereas progesterone treatment enhanced the density of BDNF+ and CC1+ oligodendrocytes in EP, SYM, and LS Wobblers. Our results suggest that BDNF could be involved in progesterone effects on motoneurons at the SYM and LS periods, whereas effects on oligodendrocytes occurred at all stages of the Wobbler disease. These steroid actions may be important to arrest the ongoing neurodegeneration. © 2011 IBRO. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v201_n_p267_Meyer http://hdl.handle.net/20.500.12110/paper_03064522_v201_n_p267_Meyer |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Brain-derived neurotrophic factor Motoneuron degeneration Neuroprotection Oligodendrocytes Progesterone Wobbler mouse brain derived neurotrophic factor progesterone animal experiment animal model animal tissue article astrocyte autoradiography controlled study disease course enzyme linked immunosorbent assay female gene expression glia cell gray matter immunohistochemistry in situ hybridization male motoneuron mouse nerve degeneration nerve fiber transport nonhuman oligodendroglia priority journal protein expression spinal cord ventral horn upregulation Age Factors Animals Brain-Derived Neurotrophic Factor Disease Models, Animal Disease Progression Enzyme-Linked Immunosorbent Assay Gene Expression Regulation Mice Mice, Neurologic Mutants Motor Neuron Disease Mutation Neuroglia Neurons Progesterone RNA, Messenger Vesicular Transport Proteins |
| spellingShingle |
Brain-derived neurotrophic factor Motoneuron degeneration Neuroprotection Oligodendrocytes Progesterone Wobbler mouse brain derived neurotrophic factor progesterone animal experiment animal model animal tissue article astrocyte autoradiography controlled study disease course enzyme linked immunosorbent assay female gene expression glia cell gray matter immunohistochemistry in situ hybridization male motoneuron mouse nerve degeneration nerve fiber transport nonhuman oligodendroglia priority journal protein expression spinal cord ventral horn upregulation Age Factors Animals Brain-Derived Neurotrophic Factor Disease Models, Animal Disease Progression Enzyme-Linked Immunosorbent Assay Gene Expression Regulation Mice Mice, Neurologic Mutants Motor Neuron Disease Mutation Neuroglia Neurons Progesterone RNA, Messenger Vesicular Transport Proteins Progesterone effects on neuronal brain-derived neurotrophic factor and glial cells during progression of Wobbler mouse neurodegeneration |
| topic_facet |
Brain-derived neurotrophic factor Motoneuron degeneration Neuroprotection Oligodendrocytes Progesterone Wobbler mouse brain derived neurotrophic factor progesterone animal experiment animal model animal tissue article astrocyte autoradiography controlled study disease course enzyme linked immunosorbent assay female gene expression glia cell gray matter immunohistochemistry in situ hybridization male motoneuron mouse nerve degeneration nerve fiber transport nonhuman oligodendroglia priority journal protein expression spinal cord ventral horn upregulation Age Factors Animals Brain-Derived Neurotrophic Factor Disease Models, Animal Disease Progression Enzyme-Linked Immunosorbent Assay Gene Expression Regulation Mice Mice, Neurologic Mutants Motor Neuron Disease Mutation Neuroglia Neurons Progesterone RNA, Messenger Vesicular Transport Proteins |
| description |
Previous results have shown a depletion of brain-derived neurotrophic factor (BDNF) mRNA in the degenerating motoneurons from clinically afflicted Wobbler mice, whereas progesterone treatment reverts this depletion. We now compared progesterone regulation of BDNF in motoneurons and oligodendrocytes of Wobbler mice at the progressive (EP, 1-3 months), symptomatic (SYM, 5-8 months old), and late stages (LS, 12-13 months). As controls we used NFR/NFR mice. Controls and Wobbler mice of different ages remained untreated or received a 20 mg progesterone pellet during 18 days. BDNF mRNA was determined in the ventral, intermediolateral, and dorsal gray matter by film autoradiography and in motoneurons using in situ hybridization. A depletion of BDNF mRNA already occurred at the EP stage of Wobblers, but progesterone was inactive at this period. In contrast, progesterone upregulated the low levels of BDNF mRNA in SYM Wobblers in the three gray matter regions analyzed. Progesterone also increased BDNF mRNA in LS Wobblers, according to grain counting procedures. BDNF protein analyzed by enzyme-linked immunosorbent assay (ELISA) in ventral horns or immunostaining of motoneurons was normal in steroid-naive SYM Wobblers. BDNF protein was decreased by progesterone, suggesting increased anterograde transport and/or release of neuronal BDNF. Wobbler mice also showed depletion of CC1-immunopositive oligodendrocytes, whereas progesterone treatment enhanced the density of BDNF+ and CC1+ oligodendrocytes in EP, SYM, and LS Wobblers. Our results suggest that BDNF could be involved in progesterone effects on motoneurons at the SYM and LS periods, whereas effects on oligodendrocytes occurred at all stages of the Wobbler disease. These steroid actions may be important to arrest the ongoing neurodegeneration. © 2011 IBRO. |
| title |
Progesterone effects on neuronal brain-derived neurotrophic factor and glial cells during progression of Wobbler mouse neurodegeneration |
| title_short |
Progesterone effects on neuronal brain-derived neurotrophic factor and glial cells during progression of Wobbler mouse neurodegeneration |
| title_full |
Progesterone effects on neuronal brain-derived neurotrophic factor and glial cells during progression of Wobbler mouse neurodegeneration |
| title_fullStr |
Progesterone effects on neuronal brain-derived neurotrophic factor and glial cells during progression of Wobbler mouse neurodegeneration |
| title_full_unstemmed |
Progesterone effects on neuronal brain-derived neurotrophic factor and glial cells during progression of Wobbler mouse neurodegeneration |
| title_sort |
progesterone effects on neuronal brain-derived neurotrophic factor and glial cells during progression of wobbler mouse neurodegeneration |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v201_n_p267_Meyer http://hdl.handle.net/20.500.12110/paper_03064522_v201_n_p267_Meyer |
| _version_ |
1768543847305969664 |