Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions

Corticotropin-releasing hormone (CRH) coordinates neuroendocrine and behavioral adaptations to stress. Acute CRH administration in vivo activates extracellular signal-regulated kinase 1/2 (ERK1/2) in limbic brain areas, acting through the CRH receptor type 1 (CRH-R1). In the present study, we used C...

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Autores principales:	Silberstein Cuña, Susana Iris, Refojo, Damián
Publicado:	2009
Materias:	CRF CRH CRH receptor ERK HPA axis mitogen activated protein kinase 1 mitogen activated protein kinase 3 acute stress amygdaloid nucleus animal experiment article brain region controlled study immunohistochemistry in situ hybridization mouse nonhuman priority journal protein expression Amphibian Proteins Amygdala Animals Autoradiography Corticosterone Corticotropin-Releasing Hormone Disease Models, Animal Gene Expression Regulation, Enzymologic Iodine Isotopes Male Mice Mice, Transgenic Mitogen-Activated Protein Kinase 3 Peptide Hormones Protein Binding Radioimmunoassay Receptors, Corticotropin-Releasing Hormone Restraint, Physical Stress, Psychological Time Factors
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v159_n2_p610_Silberstein http://hdl.handle.net/20.500.12110/paper_03064522_v159_n2_p610_Silberstein
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03064522_v159_n2_p610_Silberstein
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spelling	paper:paper_03064522_v159_n2_p610_Silberstein2023-06-08T15:31:15Z Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions Silberstein Cuña, Susana Iris Refojo, Damián CRF CRH CRH receptor ERK HPA axis mitogen activated protein kinase 1 mitogen activated protein kinase 3 acute stress amygdaloid nucleus animal experiment article brain region controlled study immunohistochemistry in situ hybridization mouse nonhuman priority journal protein expression Amphibian Proteins Amygdala Animals Autoradiography Corticosterone Corticotropin-Releasing Hormone Disease Models, Animal Gene Expression Regulation, Enzymologic Iodine Isotopes Male Mice Mice, Transgenic Mitogen-Activated Protein Kinase 3 Peptide Hormones Protein Binding Radioimmunoassay Receptors, Corticotropin-Releasing Hormone Restraint, Physical Stress, Psychological Time Factors Corticotropin-releasing hormone (CRH) coordinates neuroendocrine and behavioral adaptations to stress. Acute CRH administration in vivo activates extracellular signal-regulated kinase 1/2 (ERK1/2) in limbic brain areas, acting through the CRH receptor type 1 (CRH-R1). In the present study, we used CRH-COE-Cam mice that overexpress CRH in limbic-restricted areas, to analyze the effect of chronic CRH overexpression on ERK1/2 activation. By immunohistochemistry and confocal microscopy analysis we found that pERK1/2 levels in the basolateral amygdala (BLA) were similar in control and CRH overexpressing mice under basal conditions. Acute stress caused comparably increased levels of corticosterone in both control (CRH-COEcon-Cam) and CRH overexpressing (CRH-COEhom-Cam) animals. CRH-COEhom-Cam mice after stress showed reduced pERK1/2 immunoreactivity in the BLA compared to CRH-COEhom-Cam animals under basal conditions. Radioligand binding and in situ hybridization revealed higher density of CRH-R1 in the amygdala of CRH-COEhom mice under basal conditions compared to control littermates. A significant reduction of the receptor levels was observed in this area after acute stress, suggesting that stress may trigger CRH-R1 internalization/downregulation in these CRH overexpressing mice. Chronic CRH overexpression leads to reduced ERK1/2 activation in response to acute stress in the BLA. © 2009 IBRO. Fil:Silberstein, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Refojo, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v159_n2_p610_Silberstein http://hdl.handle.net/20.500.12110/paper_03064522_v159_n2_p610_Silberstein
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	CRF CRH CRH receptor ERK HPA axis mitogen activated protein kinase 1 mitogen activated protein kinase 3 acute stress amygdaloid nucleus animal experiment article brain region controlled study immunohistochemistry in situ hybridization mouse nonhuman priority journal protein expression Amphibian Proteins Amygdala Animals Autoradiography Corticosterone Corticotropin-Releasing Hormone Disease Models, Animal Gene Expression Regulation, Enzymologic Iodine Isotopes Male Mice Mice, Transgenic Mitogen-Activated Protein Kinase 3 Peptide Hormones Protein Binding Radioimmunoassay Receptors, Corticotropin-Releasing Hormone Restraint, Physical Stress, Psychological Time Factors
spellingShingle	CRF CRH CRH receptor ERK HPA axis mitogen activated protein kinase 1 mitogen activated protein kinase 3 acute stress amygdaloid nucleus animal experiment article brain region controlled study immunohistochemistry in situ hybridization mouse nonhuman priority journal protein expression Amphibian Proteins Amygdala Animals Autoradiography Corticosterone Corticotropin-Releasing Hormone Disease Models, Animal Gene Expression Regulation, Enzymologic Iodine Isotopes Male Mice Mice, Transgenic Mitogen-Activated Protein Kinase 3 Peptide Hormones Protein Binding Radioimmunoassay Receptors, Corticotropin-Releasing Hormone Restraint, Physical Stress, Psychological Time Factors Silberstein Cuña, Susana Iris Refojo, Damián Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
topic_facet	CRF CRH CRH receptor ERK HPA axis mitogen activated protein kinase 1 mitogen activated protein kinase 3 acute stress amygdaloid nucleus animal experiment article brain region controlled study immunohistochemistry in situ hybridization mouse nonhuman priority journal protein expression Amphibian Proteins Amygdala Animals Autoradiography Corticosterone Corticotropin-Releasing Hormone Disease Models, Animal Gene Expression Regulation, Enzymologic Iodine Isotopes Male Mice Mice, Transgenic Mitogen-Activated Protein Kinase 3 Peptide Hormones Protein Binding Radioimmunoassay Receptors, Corticotropin-Releasing Hormone Restraint, Physical Stress, Psychological Time Factors
description	Corticotropin-releasing hormone (CRH) coordinates neuroendocrine and behavioral adaptations to stress. Acute CRH administration in vivo activates extracellular signal-regulated kinase 1/2 (ERK1/2) in limbic brain areas, acting through the CRH receptor type 1 (CRH-R1). In the present study, we used CRH-COE-Cam mice that overexpress CRH in limbic-restricted areas, to analyze the effect of chronic CRH overexpression on ERK1/2 activation. By immunohistochemistry and confocal microscopy analysis we found that pERK1/2 levels in the basolateral amygdala (BLA) were similar in control and CRH overexpressing mice under basal conditions. Acute stress caused comparably increased levels of corticosterone in both control (CRH-COEcon-Cam) and CRH overexpressing (CRH-COEhom-Cam) animals. CRH-COEhom-Cam mice after stress showed reduced pERK1/2 immunoreactivity in the BLA compared to CRH-COEhom-Cam animals under basal conditions. Radioligand binding and in situ hybridization revealed higher density of CRH-R1 in the amygdala of CRH-COEhom mice under basal conditions compared to control littermates. A significant reduction of the receptor levels was observed in this area after acute stress, suggesting that stress may trigger CRH-R1 internalization/downregulation in these CRH overexpressing mice. Chronic CRH overexpression leads to reduced ERK1/2 activation in response to acute stress in the BLA. © 2009 IBRO.
author	Silberstein Cuña, Susana Iris Refojo, Damián
author_facet	Silberstein Cuña, Susana Iris Refojo, Damián
author_sort	Silberstein Cuña, Susana Iris
title	Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
title_short	Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
title_full	Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
title_fullStr	Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
title_full_unstemmed	Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
title_sort	amygdaloid perk1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v159_n2_p610_Silberstein http://hdl.handle.net/20.500.12110/paper_03064522_v159_n2_p610_Silberstein
work_keys_str_mv	AT silbersteincunasusanairis amygdaloidperk12incorticotropinreleasinghormoneoverexpressingmiceunderbasalandacutestressconditions AT refojodamian amygdaloidperk12incorticotropinreleasinghormoneoverexpressingmiceunderbasalandacutestressconditions
_version_	1768545231406366720

Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions

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