Testosterone modulates Cav2.2 calcium channels' functional expression at rat levator ani neuromuscular junction
Spinal nucleus of bulbocavernosus and its target musculature, the bulbocavernosus and levator ani muscles, are sexually dimorphic, and their sexual differentiation depends on plasmatic levels of testosterone. Electrophysiological and immunocytochemical studies have demonstrated that at mammalian adu...
Guardado en:
| Autores principales: | , , |
|---|---|
| Publicado: |
2005
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v134_n3_p817_Nudler http://hdl.handle.net/20.500.12110/paper_03064522_v134_n3_p817_Nudler |
| Aporte de: |
| id |
paper:paper_03064522_v134_n3_p817_Nudler |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_03064522_v134_n3_p817_Nudler2023-06-08T15:31:14Z Testosterone modulates Cav2.2 calcium channels' functional expression at rat levator ani neuromuscular junction Nudler, Silvana Iris Pagani, Mario Rafael Uchitel, Osvaldo Daniel ω-Conotoxin GVIA Bulbocavernosus Gonadectomy Immunostaining Synaptic transmission Testosterone propionate calcium channel calcium channel blocking agent calcium channel N type conotoxin protein subunit testosterone animal tissue article castration controlled study gonadectomy hormone action levator ani muscle male motoneuron neuromuscular synapse neuromuscular transmission neurotransmitter release nonhuman priority journal protein expression protein function protein localization rat sensitivity analysis synapse Animals Animals, Newborn Calcium Channel Blockers Calcium Channels, L-Type Calcium Channels, N-Type Diaphragm Drug Interactions Evoked Potentials Gene Expression Regulation Immunohistochemistry Male Neuromuscular Junction omega-Agatoxin IVA omega-Conotoxin GVIA Orchiectomy Pelvic Floor Radioimmunoassay Rats Rats, Sprague-Dawley Receptors, Cholinergic Testosterone Spinal nucleus of bulbocavernosus and its target musculature, the bulbocavernosus and levator ani muscles, are sexually dimorphic, and their sexual differentiation depends on plasmatic levels of testosterone. Electrophysiological and immunocytochemical studies have demonstrated that at mammalian adult neuromuscular junctions only P/Q-type Ca2+ channels (Cav2.1), mediate evoked transmitter release. Here we report that N-type Ca2+ channel (Cav2.2) blocker ω-Conotoxin GVIA, as well as Cav2.1 blocker ω-Agatoxin IVA, significantly reduced quantal content of transmitter release by ∼ 80% and ∼70% respectively at levator ani muscle of the adult rats, indicating that neuromuscular transmission is jointly mediated by both types of channels. In these synapses, we also observed that castration and restitution of plasmatic testosterone in rats resulted in changes in the sensitivity to ω-Conotoxin GVIA. Castration induced, whereas testosterone treatment avoided, functional loss of Cav2.2, as mediators of transmitter release in these synapses. Strikingly, the expression and localization of α1B subunits, which form the pore of the Cav2.2 channel, were similar at control, gonadectomized and gonadectomized testosterone-treated rats, suggesting that testosterone may regulate the coupling mechanisms between Ca v2.2 and transmitter release at the neuromuscular junctions of these sexually dimorphic motoneurons. © 2005 IBRO. Published by Elsevier Ltd. All rights reserved. Fil:Nudler, S.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pagani, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Uchitel, O.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v134_n3_p817_Nudler http://hdl.handle.net/20.500.12110/paper_03064522_v134_n3_p817_Nudler |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ω-Conotoxin GVIA Bulbocavernosus Gonadectomy Immunostaining Synaptic transmission Testosterone propionate calcium channel calcium channel blocking agent calcium channel N type conotoxin protein subunit testosterone animal tissue article castration controlled study gonadectomy hormone action levator ani muscle male motoneuron neuromuscular synapse neuromuscular transmission neurotransmitter release nonhuman priority journal protein expression protein function protein localization rat sensitivity analysis synapse Animals Animals, Newborn Calcium Channel Blockers Calcium Channels, L-Type Calcium Channels, N-Type Diaphragm Drug Interactions Evoked Potentials Gene Expression Regulation Immunohistochemistry Male Neuromuscular Junction omega-Agatoxin IVA omega-Conotoxin GVIA Orchiectomy Pelvic Floor Radioimmunoassay Rats Rats, Sprague-Dawley Receptors, Cholinergic Testosterone |
| spellingShingle |
ω-Conotoxin GVIA Bulbocavernosus Gonadectomy Immunostaining Synaptic transmission Testosterone propionate calcium channel calcium channel blocking agent calcium channel N type conotoxin protein subunit testosterone animal tissue article castration controlled study gonadectomy hormone action levator ani muscle male motoneuron neuromuscular synapse neuromuscular transmission neurotransmitter release nonhuman priority journal protein expression protein function protein localization rat sensitivity analysis synapse Animals Animals, Newborn Calcium Channel Blockers Calcium Channels, L-Type Calcium Channels, N-Type Diaphragm Drug Interactions Evoked Potentials Gene Expression Regulation Immunohistochemistry Male Neuromuscular Junction omega-Agatoxin IVA omega-Conotoxin GVIA Orchiectomy Pelvic Floor Radioimmunoassay Rats Rats, Sprague-Dawley Receptors, Cholinergic Testosterone Nudler, Silvana Iris Pagani, Mario Rafael Uchitel, Osvaldo Daniel Testosterone modulates Cav2.2 calcium channels' functional expression at rat levator ani neuromuscular junction |
| topic_facet |
ω-Conotoxin GVIA Bulbocavernosus Gonadectomy Immunostaining Synaptic transmission Testosterone propionate calcium channel calcium channel blocking agent calcium channel N type conotoxin protein subunit testosterone animal tissue article castration controlled study gonadectomy hormone action levator ani muscle male motoneuron neuromuscular synapse neuromuscular transmission neurotransmitter release nonhuman priority journal protein expression protein function protein localization rat sensitivity analysis synapse Animals Animals, Newborn Calcium Channel Blockers Calcium Channels, L-Type Calcium Channels, N-Type Diaphragm Drug Interactions Evoked Potentials Gene Expression Regulation Immunohistochemistry Male Neuromuscular Junction omega-Agatoxin IVA omega-Conotoxin GVIA Orchiectomy Pelvic Floor Radioimmunoassay Rats Rats, Sprague-Dawley Receptors, Cholinergic Testosterone |
| description |
Spinal nucleus of bulbocavernosus and its target musculature, the bulbocavernosus and levator ani muscles, are sexually dimorphic, and their sexual differentiation depends on plasmatic levels of testosterone. Electrophysiological and immunocytochemical studies have demonstrated that at mammalian adult neuromuscular junctions only P/Q-type Ca2+ channels (Cav2.1), mediate evoked transmitter release. Here we report that N-type Ca2+ channel (Cav2.2) blocker ω-Conotoxin GVIA, as well as Cav2.1 blocker ω-Agatoxin IVA, significantly reduced quantal content of transmitter release by ∼ 80% and ∼70% respectively at levator ani muscle of the adult rats, indicating that neuromuscular transmission is jointly mediated by both types of channels. In these synapses, we also observed that castration and restitution of plasmatic testosterone in rats resulted in changes in the sensitivity to ω-Conotoxin GVIA. Castration induced, whereas testosterone treatment avoided, functional loss of Cav2.2, as mediators of transmitter release in these synapses. Strikingly, the expression and localization of α1B subunits, which form the pore of the Cav2.2 channel, were similar at control, gonadectomized and gonadectomized testosterone-treated rats, suggesting that testosterone may regulate the coupling mechanisms between Ca v2.2 and transmitter release at the neuromuscular junctions of these sexually dimorphic motoneurons. © 2005 IBRO. Published by Elsevier Ltd. All rights reserved. |
| author |
Nudler, Silvana Iris Pagani, Mario Rafael Uchitel, Osvaldo Daniel |
| author_facet |
Nudler, Silvana Iris Pagani, Mario Rafael Uchitel, Osvaldo Daniel |
| author_sort |
Nudler, Silvana Iris |
| title |
Testosterone modulates Cav2.2 calcium channels' functional expression at rat levator ani neuromuscular junction |
| title_short |
Testosterone modulates Cav2.2 calcium channels' functional expression at rat levator ani neuromuscular junction |
| title_full |
Testosterone modulates Cav2.2 calcium channels' functional expression at rat levator ani neuromuscular junction |
| title_fullStr |
Testosterone modulates Cav2.2 calcium channels' functional expression at rat levator ani neuromuscular junction |
| title_full_unstemmed |
Testosterone modulates Cav2.2 calcium channels' functional expression at rat levator ani neuromuscular junction |
| title_sort |
testosterone modulates cav2.2 calcium channels' functional expression at rat levator ani neuromuscular junction |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v134_n3_p817_Nudler http://hdl.handle.net/20.500.12110/paper_03064522_v134_n3_p817_Nudler |
| work_keys_str_mv |
AT nudlersilvanairis testosteronemodulatescav22calciumchannelsfunctionalexpressionatratlevatoranineuromuscularjunction AT paganimariorafael testosteronemodulatescav22calciumchannelsfunctionalexpressionatratlevatoranineuromuscularjunction AT uchitelosvaldodaniel testosteronemodulatescav22calciumchannelsfunctionalexpressionatratlevatoranineuromuscularjunction |
| _version_ |
1768543324748120064 |