In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes

1. 1. Some in vitro studies were performed to elucidate the action of S-adenosyl-l-methionine (SAM) and thiosulphate on liver rhodanese, δ-amino-levulinic acid dehydratase (ALA-D) and cytochrome oxidase affected by cyanide in the experimental conditions. 2. 2. SAM was unable to interact with the sul...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Buzaleh, Ana María, Vázquez, Elba Susana, Batlle, Alcira María del Carmen
Publicado:	1991
Materias:	cyanide cytochrome c oxidase porphobilinogen synthase s adenosylmethionine thiosulfate thiosulfate sulfurtransferase animal tissue article controlled study enzyme activity liver homogenate mouse nonhuman priority journal Animal Cyanides Cytochrome-c Oxidase In Vitro Liver Mice Porphobilinogen Synthase S-Adenosylmethionine Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v22_n2_p281_Buzaleh http://hdl.handle.net/20.500.12110/paper_03063623_v22_n2_p281_Buzaleh
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03063623_v22_n2_p281_Buzaleh
record_format	dspace
spelling	paper:paper_03063623_v22_n2_p281_Buzaleh2023-06-08T15:31:05Z In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes Buzaleh, Ana María Vázquez, Elba Susana Batlle, Alcira María del Carmen cyanide cytochrome c oxidase porphobilinogen synthase s adenosylmethionine thiosulfate thiosulfate sulfurtransferase animal tissue article controlled study enzyme activity liver homogenate mouse nonhuman priority journal Animal Cyanides Cytochrome-c Oxidase In Vitro Liver Mice Porphobilinogen Synthase S-Adenosylmethionine Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates 1. 1. Some in vitro studies were performed to elucidate the action of S-adenosyl-l-methionine (SAM) and thiosulphate on liver rhodanese, δ-amino-levulinic acid dehydratase (ALA-D) and cytochrome oxidase affected by cyanide in the experimental conditions. 2. 2. SAM was unable to interact with the sulfur substituted rhodanese complex suggesting that SAM would blockade the thiosulphate binding sites on rhodanese. 3. 3. Cyanide and thiosulphate inhibited ALA-D activity when both compounds were present in the incubation or the preincubation mixture. Cyanide binding on the enzyme was irreversible. 4. 4. Cyanide inhibited cytochrome oxidase activity and the reversible nature of the binding was demonstrated by gel filtration. 5. 5. SAM had no effect on either ALA-D or cytochrome oxidase activities. © 1991. Fil:Buzaleh, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez, E.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del Carmen Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1991 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v22_n2_p281_Buzaleh http://hdl.handle.net/20.500.12110/paper_03063623_v22_n2_p281_Buzaleh
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cyanide cytochrome c oxidase porphobilinogen synthase s adenosylmethionine thiosulfate thiosulfate sulfurtransferase animal tissue article controlled study enzyme activity liver homogenate mouse nonhuman priority journal Animal Cyanides Cytochrome-c Oxidase In Vitro Liver Mice Porphobilinogen Synthase S-Adenosylmethionine Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates
spellingShingle	cyanide cytochrome c oxidase porphobilinogen synthase s adenosylmethionine thiosulfate thiosulfate sulfurtransferase animal tissue article controlled study enzyme activity liver homogenate mouse nonhuman priority journal Animal Cyanides Cytochrome-c Oxidase In Vitro Liver Mice Porphobilinogen Synthase S-Adenosylmethionine Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates Buzaleh, Ana María Vázquez, Elba Susana Batlle, Alcira María del Carmen In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes
topic_facet	cyanide cytochrome c oxidase porphobilinogen synthase s adenosylmethionine thiosulfate thiosulfate sulfurtransferase animal tissue article controlled study enzyme activity liver homogenate mouse nonhuman priority journal Animal Cyanides Cytochrome-c Oxidase In Vitro Liver Mice Porphobilinogen Synthase S-Adenosylmethionine Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates
description	1. 1. Some in vitro studies were performed to elucidate the action of S-adenosyl-l-methionine (SAM) and thiosulphate on liver rhodanese, δ-amino-levulinic acid dehydratase (ALA-D) and cytochrome oxidase affected by cyanide in the experimental conditions. 2. 2. SAM was unable to interact with the sulfur substituted rhodanese complex suggesting that SAM would blockade the thiosulphate binding sites on rhodanese. 3. 3. Cyanide and thiosulphate inhibited ALA-D activity when both compounds were present in the incubation or the preincubation mixture. Cyanide binding on the enzyme was irreversible. 4. 4. Cyanide inhibited cytochrome oxidase activity and the reversible nature of the binding was demonstrated by gel filtration. 5. 5. SAM had no effect on either ALA-D or cytochrome oxidase activities. © 1991.
author	Buzaleh, Ana María Vázquez, Elba Susana Batlle, Alcira María del Carmen
author_facet	Buzaleh, Ana María Vázquez, Elba Susana Batlle, Alcira María del Carmen
author_sort	Buzaleh, Ana María
title	In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes
title_short	In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes
title_full	In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes
title_fullStr	In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes
title_full_unstemmed	In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes
title_sort	in vitro effect of cyanide, thiosulphate and s-adenosyl-l-methionine on the activity of rhodanese and other enzymes
publishDate	1991
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v22_n2_p281_Buzaleh http://hdl.handle.net/20.500.12110/paper_03063623_v22_n2_p281_Buzaleh
work_keys_str_mv	AT buzalehanamaria invitroeffectofcyanidethiosulphateandsadenosyllmethionineontheactivityofrhodaneseandotherenzymes AT vazquezelbasusana invitroeffectofcyanidethiosulphateandsadenosyllmethionineontheactivityofrhodaneseandotherenzymes AT batllealciramariadelcarmen invitroeffectofcyanidethiosulphateandsadenosyllmethionineontheactivityofrhodaneseandotherenzymes
_version_	1768545188906532864

In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes

Ejemplares similares