The effect of cyanide intoxication on hepatic rhodanese kinetics
1. 1. Results of studies on the kinetics of hepatic rhodanese and the effects of S-adenosyl-l-methionine (SAM) on these kinetic parameters in cyanide-treated and non-treated mice are reported here. 2. 2. The enzyme exhibited typical Michaelis-Menten behaviour with cyanide inhibition at concentration...
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1990
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v21_n2_p219_Buzaleh http://hdl.handle.net/20.500.12110/paper_03063623_v21_n2_p219_Buzaleh |
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paper:paper_03063623_v21_n2_p219_Buzaleh2023-06-08T15:31:04Z The effect of cyanide intoxication on hepatic rhodanese kinetics Buzaleh, Ana María Vázquez, Elba Susana liver enzyme potassium cyanide s adenosylmethionine animal cell animal experiment article enzyme kinetics intoxication intraperitoneal drug administration mouse nonhuman priority journal xenobiotic metabolism Animal Cyanides Kinetics Liver Mice S-Adenosylmethionine Sulfurtransferases Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates 1. 1. Results of studies on the kinetics of hepatic rhodanese and the effects of S-adenosyl-l-methionine (SAM) on these kinetic parameters in cyanide-treated and non-treated mice are reported here. 2. 2. The enzyme exhibited typical Michaelis-Menten behaviour with cyanide inhibition at concentrations higher than 50 mM. Km values of 4.74 and 0.85 mM were obtained for thiosulphate and cyanide, respectively, in control mice. 3. 3. These results stress the biological importance of the rhodanese reaction for cyanide detoxification. 4. 4. Km values were not significantly modified when the animals were intoxicated with a lethal (20 mg/kg) or a non-lethal (4 mg/kg) dose of cyanide. 5. 5. SAM treatment either in control or in cyanide-poisoned animals doubled the Km's for cyanide. © 1990. Fil:Buzaleh, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez, E.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v21_n2_p219_Buzaleh http://hdl.handle.net/20.500.12110/paper_03063623_v21_n2_p219_Buzaleh |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
liver enzyme potassium cyanide s adenosylmethionine animal cell animal experiment article enzyme kinetics intoxication intraperitoneal drug administration mouse nonhuman priority journal xenobiotic metabolism Animal Cyanides Kinetics Liver Mice S-Adenosylmethionine Sulfurtransferases Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates |
| spellingShingle |
liver enzyme potassium cyanide s adenosylmethionine animal cell animal experiment article enzyme kinetics intoxication intraperitoneal drug administration mouse nonhuman priority journal xenobiotic metabolism Animal Cyanides Kinetics Liver Mice S-Adenosylmethionine Sulfurtransferases Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates Buzaleh, Ana María Vázquez, Elba Susana The effect of cyanide intoxication on hepatic rhodanese kinetics |
| topic_facet |
liver enzyme potassium cyanide s adenosylmethionine animal cell animal experiment article enzyme kinetics intoxication intraperitoneal drug administration mouse nonhuman priority journal xenobiotic metabolism Animal Cyanides Kinetics Liver Mice S-Adenosylmethionine Sulfurtransferases Support, Non-U.S. Gov't Thiosulfate Sulfurtransferase Thiosulfates |
| description |
1. 1. Results of studies on the kinetics of hepatic rhodanese and the effects of S-adenosyl-l-methionine (SAM) on these kinetic parameters in cyanide-treated and non-treated mice are reported here. 2. 2. The enzyme exhibited typical Michaelis-Menten behaviour with cyanide inhibition at concentrations higher than 50 mM. Km values of 4.74 and 0.85 mM were obtained for thiosulphate and cyanide, respectively, in control mice. 3. 3. These results stress the biological importance of the rhodanese reaction for cyanide detoxification. 4. 4. Km values were not significantly modified when the animals were intoxicated with a lethal (20 mg/kg) or a non-lethal (4 mg/kg) dose of cyanide. 5. 5. SAM treatment either in control or in cyanide-poisoned animals doubled the Km's for cyanide. © 1990. |
| author |
Buzaleh, Ana María Vázquez, Elba Susana |
| author_facet |
Buzaleh, Ana María Vázquez, Elba Susana |
| author_sort |
Buzaleh, Ana María |
| title |
The effect of cyanide intoxication on hepatic rhodanese kinetics |
| title_short |
The effect of cyanide intoxication on hepatic rhodanese kinetics |
| title_full |
The effect of cyanide intoxication on hepatic rhodanese kinetics |
| title_fullStr |
The effect of cyanide intoxication on hepatic rhodanese kinetics |
| title_full_unstemmed |
The effect of cyanide intoxication on hepatic rhodanese kinetics |
| title_sort |
effect of cyanide intoxication on hepatic rhodanese kinetics |
| publishDate |
1990 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v21_n2_p219_Buzaleh http://hdl.handle.net/20.500.12110/paper_03063623_v21_n2_p219_Buzaleh |
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