Potentiation of the 5-aminolevulinic acid-based photodynamic therapy with cyclophosphamide

We have investigated the efficacy of the Photodynamic Therapy (PDT) from 5-aminolevulinic acid (ALA) in combination with an antineoplastic agent using an in vitro-in vivo model developed in our laboratory. The alkylant cyclophosphamide (CY) was chosen because there is evidence of the porphyrinogenic...

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Autores principales:	Casas, Adriana Gabriela, Fukuda, Haydeé, Batlle, Alcira María del Carmen
Publicado:	1997
Materias:	5-aminolevulinic acid Cyclophosphamide Photodynamic therapy alkylating agent aminolevulinic acid cyclophosphamide photosensitizing agent porphyrin adenocarcinoma animal article Bagg albino mouse biosynthesis chemistry experimental neoplasm liver male metabolism mouse multimodality cancer therapy photochemotherapy time tissue distribution Adenocarcinoma Aminolevulinic Acid Animals Antineoplastic Agents, Alkylating Combined Modality Therapy Liver Male Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Photochemotherapy Photosensitizing Agents Porphyrins Time Factors Tissue Distribution
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03057232_v16_n1_p183_Casas http://hdl.handle.net/20.500.12110/paper_03057232_v16_n1_p183_Casas
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_03057232_v16_n1_p183_Casas2023-06-08T15:30:49Z Potentiation of the 5-aminolevulinic acid-based photodynamic therapy with cyclophosphamide Casas, Adriana Gabriela Fukuda, Haydeé Batlle, Alcira María del Carmen 5-aminolevulinic acid Cyclophosphamide Photodynamic therapy alkylating agent aminolevulinic acid cyclophosphamide photosensitizing agent porphyrin adenocarcinoma animal article Bagg albino mouse biosynthesis chemistry experimental neoplasm liver male metabolism mouse multimodality cancer therapy photochemotherapy time tissue distribution Adenocarcinoma Aminolevulinic Acid Animals Antineoplastic Agents, Alkylating Combined Modality Therapy Cyclophosphamide Liver Male Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Photochemotherapy Photosensitizing Agents Porphyrins Time Factors Tissue Distribution We have investigated the efficacy of the Photodynamic Therapy (PDT) from 5-aminolevulinic acid (ALA) in combination with an antineoplastic agent using an in vitro-in vivo model developed in our laboratory. The alkylant cyclophosphamide (CY) was chosen because there is evidence of the porphyrinogenic properties of this drug. Male BALB/c mice bearing a transplantable mammary adenoarcinoma were given two doses of 35 mg de CY/kg wt. i.p. and 9 mg/kg wt intratumorally. At 16, 22 and 40 hrs after the last injection of CY the animals were sacrificed and explants of 2 mg of tumor were incubated 2 hrs in a medium containing 0.6 mM ALA; and then irradiated with a He-Ne laser. Innocula of 1 mm3 of irradiated and nonirradiated tissue were then injected subcutaneously under the right and left flanks of a normal mouse, respectively. The efficacy of the treatment was determined following the growth of the tumor from day 10 after tumor implantation. Under the present conditions a 30% increased efficacy was observed in the case of the explants treated with CY 40 hrs after the last i.p. injection. Porphyrins in the liver and tumor and other tissues of the injected mice were also determined; except for a slight increase in tumor and liver, 40 and 22 hrs after CY i.p. injection respectively, no other changes were observed in any tissue, as compared with not CY treated mice. These results indicate that future treatment, combining the tumor localizing properties of endogenously formed porphyrins from ALA and antineoplasic drugs such as cyclophosphamide, should be encouraged. Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A.M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03057232_v16_n1_p183_Casas http://hdl.handle.net/20.500.12110/paper_03057232_v16_n1_p183_Casas
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5-aminolevulinic acid Cyclophosphamide Photodynamic therapy alkylating agent aminolevulinic acid cyclophosphamide photosensitizing agent porphyrin adenocarcinoma animal article Bagg albino mouse biosynthesis chemistry experimental neoplasm liver male metabolism mouse multimodality cancer therapy photochemotherapy time tissue distribution Adenocarcinoma Aminolevulinic Acid Animals Antineoplastic Agents, Alkylating Combined Modality Therapy Cyclophosphamide Liver Male Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Photochemotherapy Photosensitizing Agents Porphyrins Time Factors Tissue Distribution
spellingShingle	5-aminolevulinic acid Cyclophosphamide Photodynamic therapy alkylating agent aminolevulinic acid cyclophosphamide photosensitizing agent porphyrin adenocarcinoma animal article Bagg albino mouse biosynthesis chemistry experimental neoplasm liver male metabolism mouse multimodality cancer therapy photochemotherapy time tissue distribution Adenocarcinoma Aminolevulinic Acid Animals Antineoplastic Agents, Alkylating Combined Modality Therapy Cyclophosphamide Liver Male Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Photochemotherapy Photosensitizing Agents Porphyrins Time Factors Tissue Distribution Casas, Adriana Gabriela Fukuda, Haydeé Batlle, Alcira María del Carmen Potentiation of the 5-aminolevulinic acid-based photodynamic therapy with cyclophosphamide
topic_facet	5-aminolevulinic acid Cyclophosphamide Photodynamic therapy alkylating agent aminolevulinic acid cyclophosphamide photosensitizing agent porphyrin adenocarcinoma animal article Bagg albino mouse biosynthesis chemistry experimental neoplasm liver male metabolism mouse multimodality cancer therapy photochemotherapy time tissue distribution Adenocarcinoma Aminolevulinic Acid Animals Antineoplastic Agents, Alkylating Combined Modality Therapy Cyclophosphamide Liver Male Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Photochemotherapy Photosensitizing Agents Porphyrins Time Factors Tissue Distribution
description	We have investigated the efficacy of the Photodynamic Therapy (PDT) from 5-aminolevulinic acid (ALA) in combination with an antineoplastic agent using an in vitro-in vivo model developed in our laboratory. The alkylant cyclophosphamide (CY) was chosen because there is evidence of the porphyrinogenic properties of this drug. Male BALB/c mice bearing a transplantable mammary adenoarcinoma were given two doses of 35 mg de CY/kg wt. i.p. and 9 mg/kg wt intratumorally. At 16, 22 and 40 hrs after the last injection of CY the animals were sacrificed and explants of 2 mg of tumor were incubated 2 hrs in a medium containing 0.6 mM ALA; and then irradiated with a He-Ne laser. Innocula of 1 mm3 of irradiated and nonirradiated tissue were then injected subcutaneously under the right and left flanks of a normal mouse, respectively. The efficacy of the treatment was determined following the growth of the tumor from day 10 after tumor implantation. Under the present conditions a 30% increased efficacy was observed in the case of the explants treated with CY 40 hrs after the last i.p. injection. Porphyrins in the liver and tumor and other tissues of the injected mice were also determined; except for a slight increase in tumor and liver, 40 and 22 hrs after CY i.p. injection respectively, no other changes were observed in any tissue, as compared with not CY treated mice. These results indicate that future treatment, combining the tumor localizing properties of endogenously formed porphyrins from ALA and antineoplasic drugs such as cyclophosphamide, should be encouraged.
author	Casas, Adriana Gabriela Fukuda, Haydeé Batlle, Alcira María del Carmen
author_facet	Casas, Adriana Gabriela Fukuda, Haydeé Batlle, Alcira María del Carmen
author_sort	Casas, Adriana Gabriela
title	Potentiation of the 5-aminolevulinic acid-based photodynamic therapy with cyclophosphamide
title_short	Potentiation of the 5-aminolevulinic acid-based photodynamic therapy with cyclophosphamide
title_full	Potentiation of the 5-aminolevulinic acid-based photodynamic therapy with cyclophosphamide
title_fullStr	Potentiation of the 5-aminolevulinic acid-based photodynamic therapy with cyclophosphamide
title_full_unstemmed	Potentiation of the 5-aminolevulinic acid-based photodynamic therapy with cyclophosphamide
title_sort	potentiation of the 5-aminolevulinic acid-based photodynamic therapy with cyclophosphamide
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03057232_v16_n1_p183_Casas http://hdl.handle.net/20.500.12110/paper_03057232_v16_n1_p183_Casas
work_keys_str_mv	AT casasadrianagabriela potentiationofthe5aminolevulinicacidbasedphotodynamictherapywithcyclophosphamide AT fukudahaydee potentiationofthe5aminolevulinicacidbasedphotodynamictherapywithcyclophosphamide AT batllealciramariadelcarmen potentiationofthe5aminolevulinicacidbasedphotodynamictherapywithcyclophosphamide
_version_	1768546301237002240

Potentiation of the 5-aminolevulinic acid-based photodynamic therapy with cyclophosphamide

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