Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation

Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of progestins...

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Autores principales:	Bertucci, Paola Yanina, Allo, Mariano, Rocha Viegas, Luciana, Ballaré, Cecilia J., Kornblihtt, Alberto Rodolfo, Vicent, Guillermo Pablo, Pecci, Adali
Publicado:	2013
Materias:	cyclic AMP responsive element binding protein binding protein histone acetyltransferase histone acetyltransferase GCN5 histone H3 histone H4 messenger RNA progesterone receptor protein bcl x protein SWI RNA polymerase II transcription factor SNF 3' untranslated region alternative RNA splicing article Bcl x gene binding site chromatin assembly and disassembly complex formation controlled study exon gene expression regulation gene function gene induction gene location gene targeting histone acetylation human human cell intron priority journal transcription elongation transcription initiation Alternative Splicing bcl-X Protein Binding Sites Cell Line, Tumor Chromatin CREB-Binding Protein Humans p300-CBP Transcription Factors Positive Transcriptional Elongation Factor B Promegestone Receptors, Progesterone RNA Polymerase II Transcription Elongation, Genetic
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03051048_v41_n12_p6072_Bertucci http://hdl.handle.net/20.500.12110/paper_03051048_v41_n12_p6072_Bertucci
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03051048_v41_n12_p6072_Bertucci
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spelling	paper:paper_03051048_v41_n12_p6072_Bertucci2023-06-08T15:30:31Z Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation Bertucci, Paola Yanina Allo, Mariano Rocha Viegas, Luciana Ballaré, Cecilia J. Kornblihtt, Alberto Rodolfo Vicent, Guillermo Pablo Pecci, Adali cyclic AMP responsive element binding protein binding protein histone acetyltransferase histone acetyltransferase GCN5 histone H3 histone H4 messenger RNA progesterone receptor protein bcl x protein SWI RNA polymerase II transcription factor SNF 3' untranslated region alternative RNA splicing article Bcl x gene binding site chromatin assembly and disassembly complex formation controlled study exon gene expression regulation gene function gene induction gene location gene targeting histone acetylation human human cell intron priority journal transcription elongation transcription initiation Alternative Splicing bcl-X Protein Binding Sites Cell Line, Tumor Chromatin CREB-Binding Protein Humans p300-CBP Transcription Factors Positive Transcriptional Elongation Factor B Promegestone Receptors, Progesterone RNA Polymerase II Transcription Elongation, Genetic Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of progestins on the transcription of the bcl-x gene, where only intragenic progesterone receptor-binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CREB-binding protein (CBP) and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI/SNF complex. Concomitant, a more relaxed chromatin was detected along bcl-x gene mainly in the regions surrounding the intragenic PRbs. PR also mediated the recruitment of the positive elongation factor pTEFb, favoring RNA polymerase II (Pol II) elongation activity. Together these events promoted the re-distribution of the active Pol II toward the 3′-end of the gene and a decrease in the ratio between proximal and distal transcription. These results suggest a novel mechanism by which PR regulates gene expression by facilitating the proper passage of the polymerase along hormone-dependent genes. © 2013 The Author(s) 2013. Published by Oxford University Press. Fil:Bertucci, P.Y. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alló, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rocha-Viegas, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ballaré, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vicent, G.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03051048_v41_n12_p6072_Bertucci http://hdl.handle.net/20.500.12110/paper_03051048_v41_n12_p6072_Bertucci
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cyclic AMP responsive element binding protein binding protein histone acetyltransferase histone acetyltransferase GCN5 histone H3 histone H4 messenger RNA progesterone receptor protein bcl x protein SWI RNA polymerase II transcription factor SNF 3' untranslated region alternative RNA splicing article Bcl x gene binding site chromatin assembly and disassembly complex formation controlled study exon gene expression regulation gene function gene induction gene location gene targeting histone acetylation human human cell intron priority journal transcription elongation transcription initiation Alternative Splicing bcl-X Protein Binding Sites Cell Line, Tumor Chromatin CREB-Binding Protein Humans p300-CBP Transcription Factors Positive Transcriptional Elongation Factor B Promegestone Receptors, Progesterone RNA Polymerase II Transcription Elongation, Genetic
spellingShingle	cyclic AMP responsive element binding protein binding protein histone acetyltransferase histone acetyltransferase GCN5 histone H3 histone H4 messenger RNA progesterone receptor protein bcl x protein SWI RNA polymerase II transcription factor SNF 3' untranslated region alternative RNA splicing article Bcl x gene binding site chromatin assembly and disassembly complex formation controlled study exon gene expression regulation gene function gene induction gene location gene targeting histone acetylation human human cell intron priority journal transcription elongation transcription initiation Alternative Splicing bcl-X Protein Binding Sites Cell Line, Tumor Chromatin CREB-Binding Protein Humans p300-CBP Transcription Factors Positive Transcriptional Elongation Factor B Promegestone Receptors, Progesterone RNA Polymerase II Transcription Elongation, Genetic Bertucci, Paola Yanina Allo, Mariano Rocha Viegas, Luciana Ballaré, Cecilia J. Kornblihtt, Alberto Rodolfo Vicent, Guillermo Pablo Pecci, Adali Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation
topic_facet	cyclic AMP responsive element binding protein binding protein histone acetyltransferase histone acetyltransferase GCN5 histone H3 histone H4 messenger RNA progesterone receptor protein bcl x protein SWI RNA polymerase II transcription factor SNF 3' untranslated region alternative RNA splicing article Bcl x gene binding site chromatin assembly and disassembly complex formation controlled study exon gene expression regulation gene function gene induction gene location gene targeting histone acetylation human human cell intron priority journal transcription elongation transcription initiation Alternative Splicing bcl-X Protein Binding Sites Cell Line, Tumor Chromatin CREB-Binding Protein Humans p300-CBP Transcription Factors Positive Transcriptional Elongation Factor B Promegestone Receptors, Progesterone RNA Polymerase II Transcription Elongation, Genetic
description	Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of progestins on the transcription of the bcl-x gene, where only intragenic progesterone receptor-binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CREB-binding protein (CBP) and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI/SNF complex. Concomitant, a more relaxed chromatin was detected along bcl-x gene mainly in the regions surrounding the intragenic PRbs. PR also mediated the recruitment of the positive elongation factor pTEFb, favoring RNA polymerase II (Pol II) elongation activity. Together these events promoted the re-distribution of the active Pol II toward the 3′-end of the gene and a decrease in the ratio between proximal and distal transcription. These results suggest a novel mechanism by which PR regulates gene expression by facilitating the proper passage of the polymerase along hormone-dependent genes. © 2013 The Author(s) 2013. Published by Oxford University Press.
author	Bertucci, Paola Yanina Allo, Mariano Rocha Viegas, Luciana Ballaré, Cecilia J. Kornblihtt, Alberto Rodolfo Vicent, Guillermo Pablo Pecci, Adali
author_facet	Bertucci, Paola Yanina Allo, Mariano Rocha Viegas, Luciana Ballaré, Cecilia J. Kornblihtt, Alberto Rodolfo Vicent, Guillermo Pablo Pecci, Adali
author_sort	Bertucci, Paola Yanina
title	Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation
title_short	Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation
title_full	Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation
title_fullStr	Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation
title_full_unstemmed	Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation
title_sort	progesterone receptor induces bcl-x expression through intragenic binding sites favoring rna polymerase ii elongation
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03051048_v41_n12_p6072_Bertucci http://hdl.handle.net/20.500.12110/paper_03051048_v41_n12_p6072_Bertucci
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_version_	1768541561331646464

Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation

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