Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys
The major satellite DNA of the subterranean rodent Ctenomys, named RPCS, contains several consensus sequences characteristic of the U3 region of retroviral long terminal repeats (LTRs), such as a polypurine tract, CCAAT boxes, binding sites for the CC-AAT/enhancer-blnding protein (C/EBP), a TATA box...
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1994
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03051048_v22_n4_p656_Pesce http://hdl.handle.net/20.500.12110/paper_03051048_v22_n4_p656_Pesce |
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paper:paper_03051048_v22_n4_p656_Pesce2023-06-08T15:30:29Z Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys Pesce, Carlos Gustavo Rossi, Maria Susana Muro, Andrés Fernando Kornblihtt, Alberto Rodolfo binding protein deoxyribonuclease i nuclear factor nuclear factor i purine satellite dna transcription factor animal cell animal tissue article binding site consensus sequence controlled study dna binding dna footprinting enhancer region genetic variability genome in situ hybridization long terminal repeat nonhuman polyadenylation priority journal protein binding retrovirus rodent simian virus 40 tata box Animals Base Sequence DNA, Satellite DNA, Viral DNA-Binding Proteins Enhancer Elements (Genetics) Genome Molecular Sequence Data Nuclear Proteins Retroviridae Rodentia Sensitivity and Specificity Animalia Ctenomys Rodentia Simiae Simian virus Simian virus 40 unidentified retrovirus The major satellite DNA of the subterranean rodent Ctenomys, named RPCS, contains several consensus sequences characteristic of the U3 region of retroviral long terminal repeats (LTRs), such as a polypurine tract, CCAAT boxes, binding sites for the CC-AAT/enhancer-blnding protein (C/EBP), a TATA box and putative polyadenylatlon signals. RPCS presents an enormous variation in abundance between species of the same genus: while C.australls or C.talarum have approximately 3×106 copies per genome, C.opimus has none. A sequence (RPCS-I) with Identity to the SV40-enhancer core element, present In all the repeating units of the satellite is specifically protected In DNase I footprlntings. Competitions of band-shift assays with different transcription factor binding sites Indicate that binding to RPCS-I is specific and involves CCAAT proteins related to NF-1, but not to C/EBP. By the use of quantitative protein/DNA binding assays we determined that, despite of their conspicuous difference in RPCS copy number, C.talarum and C.opimus have equivalent amounts and Identical quality of RPCS-bindlng proteins. These results are consistent with the observation, by in situ hybridization, that RPCS is clustered in heterochromatic regions, where it might have restricted accessibility to transcription factors in vivo. This is the first report of the binding of transcription factors to a satellite DNA of retroviral origin. © 1994 Oxford University Press. Fil:Pesce, C.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossi, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muro, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03051048_v22_n4_p656_Pesce http://hdl.handle.net/20.500.12110/paper_03051048_v22_n4_p656_Pesce |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
binding protein deoxyribonuclease i nuclear factor nuclear factor i purine satellite dna transcription factor animal cell animal tissue article binding site consensus sequence controlled study dna binding dna footprinting enhancer region genetic variability genome in situ hybridization long terminal repeat nonhuman polyadenylation priority journal protein binding retrovirus rodent simian virus 40 tata box Animals Base Sequence DNA, Satellite DNA, Viral DNA-Binding Proteins Enhancer Elements (Genetics) Genome Molecular Sequence Data Nuclear Proteins Retroviridae Rodentia Sensitivity and Specificity Animalia Ctenomys Rodentia Simiae Simian virus Simian virus 40 unidentified retrovirus |
| spellingShingle |
binding protein deoxyribonuclease i nuclear factor nuclear factor i purine satellite dna transcription factor animal cell animal tissue article binding site consensus sequence controlled study dna binding dna footprinting enhancer region genetic variability genome in situ hybridization long terminal repeat nonhuman polyadenylation priority journal protein binding retrovirus rodent simian virus 40 tata box Animals Base Sequence DNA, Satellite DNA, Viral DNA-Binding Proteins Enhancer Elements (Genetics) Genome Molecular Sequence Data Nuclear Proteins Retroviridae Rodentia Sensitivity and Specificity Animalia Ctenomys Rodentia Simiae Simian virus Simian virus 40 unidentified retrovirus Pesce, Carlos Gustavo Rossi, Maria Susana Muro, Andrés Fernando Kornblihtt, Alberto Rodolfo Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys |
| topic_facet |
binding protein deoxyribonuclease i nuclear factor nuclear factor i purine satellite dna transcription factor animal cell animal tissue article binding site consensus sequence controlled study dna binding dna footprinting enhancer region genetic variability genome in situ hybridization long terminal repeat nonhuman polyadenylation priority journal protein binding retrovirus rodent simian virus 40 tata box Animals Base Sequence DNA, Satellite DNA, Viral DNA-Binding Proteins Enhancer Elements (Genetics) Genome Molecular Sequence Data Nuclear Proteins Retroviridae Rodentia Sensitivity and Specificity Animalia Ctenomys Rodentia Simiae Simian virus Simian virus 40 unidentified retrovirus |
| description |
The major satellite DNA of the subterranean rodent Ctenomys, named RPCS, contains several consensus sequences characteristic of the U3 region of retroviral long terminal repeats (LTRs), such as a polypurine tract, CCAAT boxes, binding sites for the CC-AAT/enhancer-blnding protein (C/EBP), a TATA box and putative polyadenylatlon signals. RPCS presents an enormous variation in abundance between species of the same genus: while C.australls or C.talarum have approximately 3×106 copies per genome, C.opimus has none. A sequence (RPCS-I) with Identity to the SV40-enhancer core element, present In all the repeating units of the satellite is specifically protected In DNase I footprlntings. Competitions of band-shift assays with different transcription factor binding sites Indicate that binding to RPCS-I is specific and involves CCAAT proteins related to NF-1, but not to C/EBP. By the use of quantitative protein/DNA binding assays we determined that, despite of their conspicuous difference in RPCS copy number, C.talarum and C.opimus have equivalent amounts and Identical quality of RPCS-bindlng proteins. These results are consistent with the observation, by in situ hybridization, that RPCS is clustered in heterochromatic regions, where it might have restricted accessibility to transcription factors in vivo. This is the first report of the binding of transcription factors to a satellite DNA of retroviral origin. © 1994 Oxford University Press. |
| author |
Pesce, Carlos Gustavo Rossi, Maria Susana Muro, Andrés Fernando Kornblihtt, Alberto Rodolfo |
| author_facet |
Pesce, Carlos Gustavo Rossi, Maria Susana Muro, Andrés Fernando Kornblihtt, Alberto Rodolfo |
| author_sort |
Pesce, Carlos Gustavo |
| title |
Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys |
| title_short |
Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys |
| title_full |
Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys |
| title_fullStr |
Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys |
| title_full_unstemmed |
Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys |
| title_sort |
binding of nuclear factors to a satellite dna of retroviral origin with marked differences in copy number among species of the rodent ctenomys |
| publishDate |
1994 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03051048_v22_n4_p656_Pesce http://hdl.handle.net/20.500.12110/paper_03051048_v22_n4_p656_Pesce |
| work_keys_str_mv |
AT pescecarlosgustavo bindingofnuclearfactorstoasatellitednaofretroviraloriginwithmarkeddifferencesincopynumberamongspeciesoftherodentctenomys AT rossimariasusana bindingofnuclearfactorstoasatellitednaofretroviraloriginwithmarkeddifferencesincopynumberamongspeciesoftherodentctenomys AT muroandresfernando bindingofnuclearfactorstoasatellitednaofretroviraloriginwithmarkeddifferencesincopynumberamongspeciesoftherodentctenomys AT kornblihttalbertorodolfo bindingofnuclearfactorstoasatellitednaofretroviraloriginwithmarkeddifferencesincopynumberamongspeciesoftherodentctenomys |
| _version_ |
1768541752834129920 |