Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis

1. 1. The effect of growing Euglena gracilis cells under light (photoheterotrophic) and dark (heterotrophic) conditions on growth, chlorophyll accumulation, ALA-S, DOVA-T activities and total soluble protein content was studied. 2. 2. Growth is greater in dark-grown cells. 3. 3. When cells previousl...

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Autores principales: Lombardo, María Elisa, Araujo, Lidia Susana, Juknat, Adela Ana
Publicado: 1988
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v91_n2_p279_Lombardo
http://hdl.handle.net/20.500.12110/paper_03050491_v91_n2_p279_Lombardo
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paper:paper_03050491_v91_n2_p279_Lombardo2023-06-08T15:30:23Z Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis Lombardo, María Elisa Araujo, Lidia Susana Juknat, Adela Ana 1. 1. The effect of growing Euglena gracilis cells under light (photoheterotrophic) and dark (heterotrophic) conditions on growth, chlorophyll accumulation, ALA-S, DOVA-T activities and total soluble protein content was studied. 2. 2. Growth is greater in dark-grown cells. 3. 3. When cells previously grown in dark are transferred to light, chlorophyll synthesis is rapidly induced, although maximal greening attained is lower than that reached when cells are grown photoheterotrophically from start. 4. 4. Ninety to ninety-five percent of total green pigment is chlorophyll α. Its increased formation with light parallels that of carotenoids. 5. 5. Both ALA-S and DOVA-T activities are greater in light-grown cells. When chloroplast development is light-induced in dark-preincubated cells shifted to light, the levels of both enzymes are also enhanced. 6. 6. Total soluble protein content of cells is greater in greening cultures and decreases with time. 7. 7. Independently of growing conditions, DOVA-T activity is always 10-15 times higher than that of ALA-S. © 1988. Fil:Lombardo, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Araujo, L.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Juknat, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1988 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v91_n2_p279_Lombardo http://hdl.handle.net/20.500.12110/paper_03050491_v91_n2_p279_Lombardo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description 1. 1. The effect of growing Euglena gracilis cells under light (photoheterotrophic) and dark (heterotrophic) conditions on growth, chlorophyll accumulation, ALA-S, DOVA-T activities and total soluble protein content was studied. 2. 2. Growth is greater in dark-grown cells. 3. 3. When cells previously grown in dark are transferred to light, chlorophyll synthesis is rapidly induced, although maximal greening attained is lower than that reached when cells are grown photoheterotrophically from start. 4. 4. Ninety to ninety-five percent of total green pigment is chlorophyll α. Its increased formation with light parallels that of carotenoids. 5. 5. Both ALA-S and DOVA-T activities are greater in light-grown cells. When chloroplast development is light-induced in dark-preincubated cells shifted to light, the levels of both enzymes are also enhanced. 6. 6. Total soluble protein content of cells is greater in greening cultures and decreases with time. 7. 7. Independently of growing conditions, DOVA-T activity is always 10-15 times higher than that of ALA-S. © 1988.
author Lombardo, María Elisa
Araujo, Lidia Susana
Juknat, Adela Ana
spellingShingle Lombardo, María Elisa
Araujo, Lidia Susana
Juknat, Adela Ana
Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis
author_facet Lombardo, María Elisa
Araujo, Lidia Susana
Juknat, Adela Ana
author_sort Lombardo, María Elisa
title Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis
title_short Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis
title_full Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis
title_fullStr Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis
title_full_unstemmed Effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in Euglena gracilis
title_sort effect of illumination on growth, chlorophyll content and δ-aminolevulinic acid synthesis in euglena gracilis
publishDate 1988
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v91_n2_p279_Lombardo
http://hdl.handle.net/20.500.12110/paper_03050491_v91_n2_p279_Lombardo
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AT araujolidiasusana effectofilluminationongrowthchlorophyllcontentanddaminolevulinicacidsynthesisineuglenagracilis
AT juknatadelaana effectofilluminationongrowthchlorophyllcontentanddaminolevulinicacidsynthesisineuglenagracilis
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