Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype

Heme biosynthesis was studied in the segregants of Saccharomyces cerevisiae (DW10 tetrade 2) from D27 and D27/C6 mating, as a function of the carbon source in the growth medium and the physiological state of the cells. The effects of the HEM R+ gene on the 5-aminolevulinate synthase (ALA-S) and 5-am...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	De Siervi, Adriana, Rossetti, María Victoria, Batlle, Alcira María del Carmen
Publicado:	1996
Materias:	5-aminolevulinate dehydratase 5-aminolevulinate synthase catabolite repression and de-repression HEM R+ mutants heme biosynthesis intracellular 5-aminolevulinate acid porphobilinogen-deaminase Saccharomyces cerevisiae heme article heme synthesis mutant phenotype priority journal
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v115_n2_p167_DeSiervi http://hdl.handle.net/20.500.12110/paper_03050491_v115_n2_p167_DeSiervi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03050491_v115_n2_p167_DeSiervi
record_format	dspace
spelling	paper:paper_03050491_v115_n2_p167_DeSiervi2023-06-08T15:30:18Z Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype De Siervi, Adriana Rossetti, María Victoria Batlle, Alcira María del Carmen 5-aminolevulinate dehydratase 5-aminolevulinate synthase catabolite repression and de-repression HEM R+ mutants heme biosynthesis intracellular 5-aminolevulinate acid porphobilinogen-deaminase Saccharomyces cerevisiae heme article heme synthesis mutant phenotype priority journal Saccharomyces cerevisiae Saccharomyces cerevisiae Heme biosynthesis was studied in the segregants of Saccharomyces cerevisiae (DW10 tetrade 2) from D27 and D27/C6 mating, as a function of the carbon source in the growth medium and the physiological state of the cells. The effects of the HEM R+ gene on the 5-aminolevulinate synthase (ALA-S) and 5-aminolevulinate dehydratase (ALA-D) activities of heme biosynthesis in cells grown on nonfermentable and fermentable carbon sources were compared. Profiles obtained for both strains grown on a fermentable carbon source (glucose) were identical. However, in the presence of a nonfermentable carbon source (ethanol), they behave quite differently, as if the mutation could only be expressed under these growth conditions. Moreover, their behavior is similar to that found for the parental strains, indicating that for the mutant its particular behavior might be inheritedly linked to the HEM R+ gene, which in turn affects some regulatory aspects of ALA synthesis explaining its characteristic phenotype. Fil:De Siervi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A.M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v115_n2_p167_DeSiervi http://hdl.handle.net/20.500.12110/paper_03050491_v115_n2_p167_DeSiervi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5-aminolevulinate dehydratase 5-aminolevulinate synthase catabolite repression and de-repression HEM R+ mutants heme biosynthesis intracellular 5-aminolevulinate acid porphobilinogen-deaminase Saccharomyces cerevisiae heme article heme synthesis mutant phenotype priority journal Saccharomyces cerevisiae Saccharomyces cerevisiae
spellingShingle	5-aminolevulinate dehydratase 5-aminolevulinate synthase catabolite repression and de-repression HEM R+ mutants heme biosynthesis intracellular 5-aminolevulinate acid porphobilinogen-deaminase Saccharomyces cerevisiae heme article heme synthesis mutant phenotype priority journal Saccharomyces cerevisiae Saccharomyces cerevisiae De Siervi, Adriana Rossetti, María Victoria Batlle, Alcira María del Carmen Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
topic_facet	5-aminolevulinate dehydratase 5-aminolevulinate synthase catabolite repression and de-repression HEM R+ mutants heme biosynthesis intracellular 5-aminolevulinate acid porphobilinogen-deaminase Saccharomyces cerevisiae heme article heme synthesis mutant phenotype priority journal Saccharomyces cerevisiae Saccharomyces cerevisiae
description	Heme biosynthesis was studied in the segregants of Saccharomyces cerevisiae (DW10 tetrade 2) from D27 and D27/C6 mating, as a function of the carbon source in the growth medium and the physiological state of the cells. The effects of the HEM R+ gene on the 5-aminolevulinate synthase (ALA-S) and 5-aminolevulinate dehydratase (ALA-D) activities of heme biosynthesis in cells grown on nonfermentable and fermentable carbon sources were compared. Profiles obtained for both strains grown on a fermentable carbon source (glucose) were identical. However, in the presence of a nonfermentable carbon source (ethanol), they behave quite differently, as if the mutation could only be expressed under these growth conditions. Moreover, their behavior is similar to that found for the parental strains, indicating that for the mutant its particular behavior might be inheritedly linked to the HEM R+ gene, which in turn affects some regulatory aspects of ALA synthesis explaining its characteristic phenotype.
author	De Siervi, Adriana Rossetti, María Victoria Batlle, Alcira María del Carmen
author_facet	De Siervi, Adriana Rossetti, María Victoria Batlle, Alcira María del Carmen
author_sort	De Siervi, Adriana
title	Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
title_short	Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
title_full	Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
title_fullStr	Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
title_full_unstemmed	Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
title_sort	porphyrin biosynthesis in normal and haem mutants of saccharomyces cerevisiae. studies on the inheritance of the hem r+ phenotype
publishDate	1996
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v115_n2_p167_DeSiervi http://hdl.handle.net/20.500.12110/paper_03050491_v115_n2_p167_DeSiervi
work_keys_str_mv	AT desierviadriana porphyrinbiosynthesisinnormalandhaemmutantsofsaccharomycescerevisiaestudiesontheinheritanceofthehemrphenotype AT rossettimariavictoria porphyrinbiosynthesisinnormalandhaemmutantsofsaccharomycescerevisiaestudiesontheinheritanceofthehemrphenotype AT batllealciramariadelcarmen porphyrinbiosynthesisinnormalandhaemmutantsofsaccharomycescerevisiaestudiesontheinheritanceofthehemrphenotype
_version_	1768543369386000384

Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype

Ejemplares similares