Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus
The effect of glycoprotein processing and transport on Junin virus (JV) maturation was studied by using brefeldin A (BFA) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), two inhibitors affecting different steps of the intracellular exocytic pathway, combined with low temperature incubation. Bot...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v142_n11_p2179_Candurra http://hdl.handle.net/20.500.12110/paper_03048608_v142_n11_p2179_Candurra |
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paper:paper_03048608_v142_n11_p2179_Candurra2023-06-08T15:30:03Z Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus Candurra, Nélida Alicia Damonte, Elsa Beatriz antiinfective agent antivirus agent brefeldin A carbonyl cyanide chlorophenylhydrazone cyclopentane derivative glycoprotein macrolide protein synthesis inhibitor proteinase virus envelope protein animal article cell fractionation Cercopithecus drug effect exocytosis growth, development and aging Junin virus metabolism physiology protein processing rabbit Vero cell Animals Anti-Bacterial Agents Antiviral Agents Brefeldin A Carbonyl Cyanide m-Chlorophenyl Hydrazone Cercopithecus aethiops Cyclopentanes Endopeptidases Exocytosis Glycoproteins Junin virus Macrolides Protein Processing, Post-Translational Protein Synthesis Inhibitors Rabbits Subcellular Fractions Vero Cells Viral Envelope Proteins Animalia Cercopithecus Junin virus Oryctolagus cuniculus The effect of glycoprotein processing and transport on Junin virus (JV) maturation was studied by using brefeldin A (BFA) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), two inhibitors affecting different steps of the intracellular exocytic pathway, combined with low temperature incubation. Both compounds inhibited the multiplication of JV, strain IV4454, in Vero cells in a dose dependent manner. The addition of the compounds after several cycles of infection for a very short treatment period produced an immediate arrest on the formation of JV infectious particles, due to their effect on a late event in JV infective cycle. Extracellular and cell-associated virus yields were reduced to the same extent, suggesting that the assembly of virus particles was the blocked stage. In infected cells treated with CCCP and BFA an altered subcellular distribution of partially processed viral glycoproteins was observed, with an accumulation of JV glycoproteins at the endoplasmic reticulum and a blockade of their transport to the site of envelopment in the plasma membrane. Concomitantly, the cleavage of the precursor GPC to the mature glycoprotein GP38 was strongly inhibited when the exocytic pathway was affected. Thus, results obtained with BFA allow to conclude that the proteolytic processing of GPC probably occurs at the trans-Golgi network and this cleavage together with the glycoprotein presence at the cell surface is required for maturation of infectious JV particles. Fil:Candurra, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v142_n11_p2179_Candurra http://hdl.handle.net/20.500.12110/paper_03048608_v142_n11_p2179_Candurra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
antiinfective agent antivirus agent brefeldin A carbonyl cyanide chlorophenylhydrazone cyclopentane derivative glycoprotein macrolide protein synthesis inhibitor proteinase virus envelope protein animal article cell fractionation Cercopithecus drug effect exocytosis growth, development and aging Junin virus metabolism physiology protein processing rabbit Vero cell Animals Anti-Bacterial Agents Antiviral Agents Brefeldin A Carbonyl Cyanide m-Chlorophenyl Hydrazone Cercopithecus aethiops Cyclopentanes Endopeptidases Exocytosis Glycoproteins Junin virus Macrolides Protein Processing, Post-Translational Protein Synthesis Inhibitors Rabbits Subcellular Fractions Vero Cells Viral Envelope Proteins Animalia Cercopithecus Junin virus Oryctolagus cuniculus |
| spellingShingle |
antiinfective agent antivirus agent brefeldin A carbonyl cyanide chlorophenylhydrazone cyclopentane derivative glycoprotein macrolide protein synthesis inhibitor proteinase virus envelope protein animal article cell fractionation Cercopithecus drug effect exocytosis growth, development and aging Junin virus metabolism physiology protein processing rabbit Vero cell Animals Anti-Bacterial Agents Antiviral Agents Brefeldin A Carbonyl Cyanide m-Chlorophenyl Hydrazone Cercopithecus aethiops Cyclopentanes Endopeptidases Exocytosis Glycoproteins Junin virus Macrolides Protein Processing, Post-Translational Protein Synthesis Inhibitors Rabbits Subcellular Fractions Vero Cells Viral Envelope Proteins Animalia Cercopithecus Junin virus Oryctolagus cuniculus Candurra, Nélida Alicia Damonte, Elsa Beatriz Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus |
| topic_facet |
antiinfective agent antivirus agent brefeldin A carbonyl cyanide chlorophenylhydrazone cyclopentane derivative glycoprotein macrolide protein synthesis inhibitor proteinase virus envelope protein animal article cell fractionation Cercopithecus drug effect exocytosis growth, development and aging Junin virus metabolism physiology protein processing rabbit Vero cell Animals Anti-Bacterial Agents Antiviral Agents Brefeldin A Carbonyl Cyanide m-Chlorophenyl Hydrazone Cercopithecus aethiops Cyclopentanes Endopeptidases Exocytosis Glycoproteins Junin virus Macrolides Protein Processing, Post-Translational Protein Synthesis Inhibitors Rabbits Subcellular Fractions Vero Cells Viral Envelope Proteins Animalia Cercopithecus Junin virus Oryctolagus cuniculus |
| description |
The effect of glycoprotein processing and transport on Junin virus (JV) maturation was studied by using brefeldin A (BFA) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), two inhibitors affecting different steps of the intracellular exocytic pathway, combined with low temperature incubation. Both compounds inhibited the multiplication of JV, strain IV4454, in Vero cells in a dose dependent manner. The addition of the compounds after several cycles of infection for a very short treatment period produced an immediate arrest on the formation of JV infectious particles, due to their effect on a late event in JV infective cycle. Extracellular and cell-associated virus yields were reduced to the same extent, suggesting that the assembly of virus particles was the blocked stage. In infected cells treated with CCCP and BFA an altered subcellular distribution of partially processed viral glycoproteins was observed, with an accumulation of JV glycoproteins at the endoplasmic reticulum and a blockade of their transport to the site of envelopment in the plasma membrane. Concomitantly, the cleavage of the precursor GPC to the mature glycoprotein GP38 was strongly inhibited when the exocytic pathway was affected. Thus, results obtained with BFA allow to conclude that the proteolytic processing of GPC probably occurs at the trans-Golgi network and this cleavage together with the glycoprotein presence at the cell surface is required for maturation of infectious JV particles. |
| author |
Candurra, Nélida Alicia Damonte, Elsa Beatriz |
| author_facet |
Candurra, Nélida Alicia Damonte, Elsa Beatriz |
| author_sort |
Candurra, Nélida Alicia |
| title |
Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus |
| title_short |
Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus |
| title_full |
Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus |
| title_fullStr |
Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus |
| title_full_unstemmed |
Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus |
| title_sort |
effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of junin virus |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v142_n11_p2179_Candurra http://hdl.handle.net/20.500.12110/paper_03048608_v142_n11_p2179_Candurra |
| work_keys_str_mv |
AT candurranelidaalicia effectofinhibitorsoftheintracellularexocyticpathwayonglycoproteinprocessingandmaturationofjuninvirus AT damonteelsabeatriz effectofinhibitorsoftheintracellularexocyticpathwayonglycoproteinprocessingandmaturationofjuninvirus |
| _version_ |
1768542645634727936 |