The entry of Junin virus into Vero cells

The entry mechanism of Junin virus (JV) into Vero cells was studied analyzing the effect of lysosomotropic compounds and acid pH on JV infection. Ammonium chloride, amantadine, chlorpheniramine and procaine inhibited JV production. The action of ammonium chloride was exerted at early times of infect...

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Autores principales:	Castilla, Viviana, Mersich, Susana E., Candurra, Nélida Alicia, Damonte, Elsa Beatriz
Publicado:	1994
Materias:	amantadine ammonium chloride chlorpheniramine procaine virus protein animal article biosynthesis drug effect fluorescent antibody technique Junin virus membrane fusion pH physiology Vero cell virus replication Amantadine Ammonium Chloride Animal Chlorpheniramine Fluorescent Antibody Technique Hydrogen-Ion Concentration Membrane Fusion Procaine Support, Non-U.S. Gov't Vero Cells Viral Proteins Virus Replication
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v136_n3-4_p363_Castilla http://hdl.handle.net/20.500.12110/paper_03048608_v136_n3-4_p363_Castilla
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03048608_v136_n3-4_p363_Castilla
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spelling	paper:paper_03048608_v136_n3-4_p363_Castilla2023-06-08T15:30:01Z The entry of Junin virus into Vero cells Castilla, Viviana Mersich, Susana E. Candurra, Nélida Alicia Damonte, Elsa Beatriz amantadine ammonium chloride chlorpheniramine procaine virus protein animal article biosynthesis drug effect fluorescent antibody technique Junin virus membrane fusion pH physiology Vero cell virus replication Amantadine Ammonium Chloride Animal Chlorpheniramine Fluorescent Antibody Technique Hydrogen-Ion Concentration Junin virus Membrane Fusion Procaine Support, Non-U.S. Gov't Vero Cells Viral Proteins Virus Replication The entry mechanism of Junin virus (JV) into Vero cells was studied analyzing the effect of lysosomotropic compounds and acid pH on JV infection. Ammonium chloride, amantadine, chlorpheniramine and procaine inhibited JV production. The action of ammonium chloride was exerted at early times of infection. Virus internalization was inhibited and viral protein expression was not detected. When the extracellular medium was buffered at low pH, the ammonium chloride induced block on JV infection was overcome. Furthermore, JV was able to induce fusion of infected cells at pH 5.5 leading to polykaryoctye formation. Taken together, these results demonstrate that JV entry occurs through an endocytic mechanism requiring a low pH dependent membrane fusion. © 1994 Springer-Verlag. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mersich, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Candurra, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v136_n3-4_p363_Castilla http://hdl.handle.net/20.500.12110/paper_03048608_v136_n3-4_p363_Castilla
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	amantadine ammonium chloride chlorpheniramine procaine virus protein animal article biosynthesis drug effect fluorescent antibody technique Junin virus membrane fusion pH physiology Vero cell virus replication Amantadine Ammonium Chloride Animal Chlorpheniramine Fluorescent Antibody Technique Hydrogen-Ion Concentration Junin virus Membrane Fusion Procaine Support, Non-U.S. Gov't Vero Cells Viral Proteins Virus Replication
spellingShingle	amantadine ammonium chloride chlorpheniramine procaine virus protein animal article biosynthesis drug effect fluorescent antibody technique Junin virus membrane fusion pH physiology Vero cell virus replication Amantadine Ammonium Chloride Animal Chlorpheniramine Fluorescent Antibody Technique Hydrogen-Ion Concentration Junin virus Membrane Fusion Procaine Support, Non-U.S. Gov't Vero Cells Viral Proteins Virus Replication Castilla, Viviana Mersich, Susana E. Candurra, Nélida Alicia Damonte, Elsa Beatriz The entry of Junin virus into Vero cells
topic_facet	amantadine ammonium chloride chlorpheniramine procaine virus protein animal article biosynthesis drug effect fluorescent antibody technique Junin virus membrane fusion pH physiology Vero cell virus replication Amantadine Ammonium Chloride Animal Chlorpheniramine Fluorescent Antibody Technique Hydrogen-Ion Concentration Junin virus Membrane Fusion Procaine Support, Non-U.S. Gov't Vero Cells Viral Proteins Virus Replication
description	The entry mechanism of Junin virus (JV) into Vero cells was studied analyzing the effect of lysosomotropic compounds and acid pH on JV infection. Ammonium chloride, amantadine, chlorpheniramine and procaine inhibited JV production. The action of ammonium chloride was exerted at early times of infection. Virus internalization was inhibited and viral protein expression was not detected. When the extracellular medium was buffered at low pH, the ammonium chloride induced block on JV infection was overcome. Furthermore, JV was able to induce fusion of infected cells at pH 5.5 leading to polykaryoctye formation. Taken together, these results demonstrate that JV entry occurs through an endocytic mechanism requiring a low pH dependent membrane fusion. © 1994 Springer-Verlag.
author	Castilla, Viviana Mersich, Susana E. Candurra, Nélida Alicia Damonte, Elsa Beatriz
author_facet	Castilla, Viviana Mersich, Susana E. Candurra, Nélida Alicia Damonte, Elsa Beatriz
author_sort	Castilla, Viviana
title	The entry of Junin virus into Vero cells
title_short	The entry of Junin virus into Vero cells
title_full	The entry of Junin virus into Vero cells
title_fullStr	The entry of Junin virus into Vero cells
title_full_unstemmed	The entry of Junin virus into Vero cells
title_sort	entry of junin virus into vero cells
publishDate	1994
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v136_n3-4_p363_Castilla http://hdl.handle.net/20.500.12110/paper_03048608_v136_n3-4_p363_Castilla
work_keys_str_mv	AT castillaviviana theentryofjuninvirusintoverocells AT mersichsusanae theentryofjuninvirusintoverocells AT candurranelidaalicia theentryofjuninvirusintoverocells AT damonteelsabeatriz theentryofjuninvirusintoverocells AT castillaviviana entryofjuninvirusintoverocells AT mersichsusanae entryofjuninvirusintoverocells AT candurranelidaalicia entryofjuninvirusintoverocells AT damonteelsabeatriz entryofjuninvirusintoverocells
_version_	1768542939616641024

The entry of Junin virus into Vero cells

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