Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells

Soybean callus δ-aminolaevulinate synthetase (ALA-S) has been covalently attached to Sepharose 4B. The optimal conditions for binding have been determined. The water-insoluble ALA-S retained 40% of the activity of the original soluble preparation, the coupling yield was also high. Sepharose - ALA-S...

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Detalles Bibliográficos
Autores principales: Wider de Xifra, Eva Adela, Stella de Rosellini, Ana María Cristina, Batlle, Alcira María del Carmen
Publicado: 1978
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044211_v11_n2_p93_WiderdeXifra
http://hdl.handle.net/20.500.12110/paper_03044211_v11_n2_p93_WiderdeXifra
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03044211_v11_n2_p93_WiderdeXifra
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spelling paper:paper_03044211_v11_n2_p93_WiderdeXifra2023-06-08T15:29:59Z Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells Wider de Xifra, Eva Adela Stella de Rosellini, Ana María Cristina Batlle, Alcira María del Carmen Soybean callus δ-aminolaevulinate synthetase (ALA-S) has been covalently attached to Sepharose 4B. The optimal conditions for binding have been determined. The water-insoluble ALA-S retained 40% of the activity of the original soluble preparation, the coupling yield was also high. Sepharose - ALA-S could be stored at 4°C for periods up to 40 days with only 25% loss of activity and it could be repeatedly used with little alteration of its enzymic activity. pH optima of the free and bound enzyme were the same. © 1978. Fil:Wider de Xifra, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stella, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1978 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044211_v11_n2_p93_WiderdeXifra http://hdl.handle.net/20.500.12110/paper_03044211_v11_n2_p93_WiderdeXifra
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description Soybean callus δ-aminolaevulinate synthetase (ALA-S) has been covalently attached to Sepharose 4B. The optimal conditions for binding have been determined. The water-insoluble ALA-S retained 40% of the activity of the original soluble preparation, the coupling yield was also high. Sepharose - ALA-S could be stored at 4°C for periods up to 40 days with only 25% loss of activity and it could be repeatedly used with little alteration of its enzymic activity. pH optima of the free and bound enzyme were the same. © 1978.
author Wider de Xifra, Eva Adela
Stella de Rosellini, Ana María Cristina
Batlle, Alcira María del Carmen
spellingShingle Wider de Xifra, Eva Adela
Stella de Rosellini, Ana María Cristina
Batlle, Alcira María del Carmen
Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells
author_facet Wider de Xifra, Eva Adela
Stella de Rosellini, Ana María Cristina
Batlle, Alcira María del Carmen
author_sort Wider de Xifra, Eva Adela
title Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells
title_short Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells
title_full Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells
title_fullStr Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells
title_full_unstemmed Porphyrin biosynthesis-immobilized enzymes and ligands. IX. Studies on δ-aminolaevulinate synthetase from cultured soybean cells
title_sort porphyrin biosynthesis-immobilized enzymes and ligands. ix. studies on δ-aminolaevulinate synthetase from cultured soybean cells
publishDate 1978
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044211_v11_n2_p93_WiderdeXifra
http://hdl.handle.net/20.500.12110/paper_03044211_v11_n2_p93_WiderdeXifra
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AT stelladerosellinianamariacristina porphyrinbiosynthesisimmobilizedenzymesandligandsixstudiesondaminolaevulinatesynthetasefromculturedsoybeancells
AT batllealciramariadelcarmen porphyrinbiosynthesisimmobilizedenzymesandligandsixstudiesondaminolaevulinatesynthetasefromculturedsoybeancells
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