Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis
Highly stable selective adsorbents for δ-aminolaevulinate dehydratase (ALA-D) were prepared by attaching the substrate to agarose beads, either directly or through an extension arm. Columns containing these biospecific adsorbents can completely bind the enzyme present in extracts of Euglena gracilis...
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1978
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044211_v11_n2_p87_Stella http://hdl.handle.net/20.500.12110/paper_03044211_v11_n2_p87_Stella |
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paper:paper_03044211_v11_n2_p87_Stella2023-06-08T15:29:59Z Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis Stella de Rosellini, Ana María Cristina Batlle, Alcira María del Carmen Highly stable selective adsorbents for δ-aminolaevulinate dehydratase (ALA-D) were prepared by attaching the substrate to agarose beads, either directly or through an extension arm. Columns containing these biospecific adsorbents can completely bind the enzyme present in extracts of Euglena gracilis Z strain. Elution is obtained by changing the ionic strength of the buffer. Due to their ease of preparation and high stability these adsorbents may be of general value for purification of ALA-D from different sources. © 1978. Fil:Stella, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1978 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044211_v11_n2_p87_Stella http://hdl.handle.net/20.500.12110/paper_03044211_v11_n2_p87_Stella |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
description |
Highly stable selective adsorbents for δ-aminolaevulinate dehydratase (ALA-D) were prepared by attaching the substrate to agarose beads, either directly or through an extension arm. Columns containing these biospecific adsorbents can completely bind the enzyme present in extracts of Euglena gracilis Z strain. Elution is obtained by changing the ionic strength of the buffer. Due to their ease of preparation and high stability these adsorbents may be of general value for purification of ALA-D from different sources. © 1978. |
author |
Stella de Rosellini, Ana María Cristina Batlle, Alcira María del Carmen |
spellingShingle |
Stella de Rosellini, Ana María Cristina Batlle, Alcira María del Carmen Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis |
author_facet |
Stella de Rosellini, Ana María Cristina Batlle, Alcira María del Carmen |
author_sort |
Stella de Rosellini, Ana María Cristina |
title |
Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis |
title_short |
Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis |
title_full |
Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis |
title_fullStr |
Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis |
title_full_unstemmed |
Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis |
title_sort |
porphyrin biosynthesis-immobilized enzymes and ligands viii. studies on the purification of δ-aminolaevulinate dehydratase from euglena gracilis |
publishDate |
1978 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044211_v11_n2_p87_Stella http://hdl.handle.net/20.500.12110/paper_03044211_v11_n2_p87_Stella |
work_keys_str_mv |
AT stelladerosellinianamariacristina porphyrinbiosynthesisimmobilizedenzymesandligandsviiistudiesonthepurificationofdaminolaevulinatedehydratasefromeuglenagracilis AT batllealciramariadelcarmen porphyrinbiosynthesisimmobilizedenzymesandligandsviiistudiesonthepurificationofdaminolaevulinatedehydratasefromeuglenagracilis |
_version_ |
1768543035207974912 |