Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis

Highly stable selective adsorbents for δ-aminolaevulinate dehydratase (ALA-D) were prepared by attaching the substrate to agarose beads, either directly or through an extension arm. Columns containing these biospecific adsorbents can completely bind the enzyme present in extracts of Euglena gracilis...

Descripción completa

Detalles Bibliográficos
Autores principales: Stella de Rosellini, Ana María Cristina, Batlle, Alcira María del Carmen
Publicado: 1978
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044211_v11_n2_p87_Stella
http://hdl.handle.net/20.500.12110/paper_03044211_v11_n2_p87_Stella
Aporte de:
id paper:paper_03044211_v11_n2_p87_Stella
record_format dspace
spelling paper:paper_03044211_v11_n2_p87_Stella2023-06-08T15:29:59Z Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis Stella de Rosellini, Ana María Cristina Batlle, Alcira María del Carmen Highly stable selective adsorbents for δ-aminolaevulinate dehydratase (ALA-D) were prepared by attaching the substrate to agarose beads, either directly or through an extension arm. Columns containing these biospecific adsorbents can completely bind the enzyme present in extracts of Euglena gracilis Z strain. Elution is obtained by changing the ionic strength of the buffer. Due to their ease of preparation and high stability these adsorbents may be of general value for purification of ALA-D from different sources. © 1978. Fil:Stella, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1978 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044211_v11_n2_p87_Stella http://hdl.handle.net/20.500.12110/paper_03044211_v11_n2_p87_Stella
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description Highly stable selective adsorbents for δ-aminolaevulinate dehydratase (ALA-D) were prepared by attaching the substrate to agarose beads, either directly or through an extension arm. Columns containing these biospecific adsorbents can completely bind the enzyme present in extracts of Euglena gracilis Z strain. Elution is obtained by changing the ionic strength of the buffer. Due to their ease of preparation and high stability these adsorbents may be of general value for purification of ALA-D from different sources. © 1978.
author Stella de Rosellini, Ana María Cristina
Batlle, Alcira María del Carmen
spellingShingle Stella de Rosellini, Ana María Cristina
Batlle, Alcira María del Carmen
Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis
author_facet Stella de Rosellini, Ana María Cristina
Batlle, Alcira María del Carmen
author_sort Stella de Rosellini, Ana María Cristina
title Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis
title_short Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis
title_full Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis
title_fullStr Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis
title_full_unstemmed Porphyrin biosynthesis-immobilized enzymes and ligands VIII. Studies on the purification of δ-aminolaevulinate dehydratase from Euglena gracilis
title_sort porphyrin biosynthesis-immobilized enzymes and ligands viii. studies on the purification of δ-aminolaevulinate dehydratase from euglena gracilis
publishDate 1978
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044211_v11_n2_p87_Stella
http://hdl.handle.net/20.500.12110/paper_03044211_v11_n2_p87_Stella
work_keys_str_mv AT stelladerosellinianamariacristina porphyrinbiosynthesisimmobilizedenzymesandligandsviiistudiesonthepurificationofdaminolaevulinatedehydratasefromeuglenagracilis
AT batllealciramariadelcarmen porphyrinbiosynthesisimmobilizedenzymesandligandsviiistudiesonthepurificationofdaminolaevulinatedehydratasefromeuglenagracilis
_version_ 1768543035207974912