Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead

In the present work we have been able to demonstrate the phenobarbital and lead exert an inducing effect on the biosynthesis of δ-aminovulenic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes of normal adult rats. Dibutyryl cyclic AMP enhances the induction...

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Detalles Bibliográficos
Autores principales: Cánepa, Eduardo Tomás, Llambías, Elena Blanca Cecilia, Grinstein, Moisés
Publicado: 1985
Materias:
(Rat hepatocyte)
Cytochrome P-450
Enzyme induction
Ferrochelatase
Glucose
δ-Aminolevulinate synthase
5 aminolevulinate synthase
adrenalin
bucladesine
cycloheximide
cytochrome p450
ferrochelatase
glucose
insulin
lead
phenobarbital
dose response
dose time effect relation
drug response
in vitro study
liver
liver cell
nonhuman
priority journal
5-Aminolevulinate Synthetase
Animal
Bucladesine
Cells, Cultured
Cycloheximide
Cytochrome P-450 Enzyme System
Enzyme Induction
Epinephrine
Hemeproteins
Insulin
Lead
Liver
Lyases
Male
Phenobarbital
Rats
Subcellular Fractions
Support, Non-U.S. Gov't
Theophylline
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v841_n2_p186_Canepa
http://hdl.handle.net/20.500.12110/paper_03044165_v841_n2_p186_Canepa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03044165_v841_n2_p186_Canepa
record_format dspace
spelling paper:paper_03044165_v841_n2_p186_Canepa2023-06-08T15:29:58Z Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead Cánepa, Eduardo Tomás Llambías, Elena Blanca Cecilia Grinstein, Moisés (Rat hepatocyte) Cytochrome P-450 Enzyme induction Ferrochelatase Glucose δ-Aminolevulinate synthase 5 aminolevulinate synthase adrenalin bucladesine cycloheximide cytochrome p450 ferrochelatase glucose insulin lead phenobarbital dose response dose time effect relation drug response in vitro study liver liver cell nonhuman priority journal 5-Aminolevulinate Synthetase Animal Bucladesine Cells, Cultured Cycloheximide Cytochrome P-450 Enzyme System Enzyme Induction Epinephrine Ferrochelatase Glucose Hemeproteins Insulin Lead Liver Lyases Male Phenobarbital Rats Subcellular Fractions Support, Non-U.S. Gov't Theophylline In the present work we have been able to demonstrate the phenobarbital and lead exert an inducing effect on the biosynthesis of δ-aminovulenic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes of normal adult rats. Dibutyryl cyclic AMP enhances the induction effect produced by phenobarbital in this in vitro system. Glucose inhibits the induction of δ-aminolevulinic acid synthase and ferrochelatase. This repression effect can be reversed with increasing concentrations of dibutyryl cyclic AMP. No glucose effect was observed on the phenobarbital- and lead-mediated inductions of cytochrome P-450. The present results add more experimental evidence to support the concept that the last enzyme of the heme pathway is inducible, and as such may have a significant role in regulatory mechanisms of porphyrin and heme biosynthesis. © 1985. Fil:Canepa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llambias, E.B.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v841_n2_p186_Canepa http://hdl.handle.net/20.500.12110/paper_03044165_v841_n2_p186_Canepa
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic (Rat hepatocyte)
Cytochrome P-450
Enzyme induction
Ferrochelatase
Glucose
δ-Aminolevulinate synthase
5 aminolevulinate synthase
adrenalin
bucladesine
cycloheximide
cytochrome p450
ferrochelatase
glucose
insulin
lead
phenobarbital
dose response
dose time effect relation
drug response
in vitro study
liver
liver cell
nonhuman
priority journal
5-Aminolevulinate Synthetase
Animal
Bucladesine
Cells, Cultured
Cycloheximide
Cytochrome P-450 Enzyme System
Enzyme Induction
Epinephrine
Ferrochelatase
Glucose
Hemeproteins
Insulin
Lead
Liver
Lyases
Male
Phenobarbital
Rats
Subcellular Fractions
Support, Non-U.S. Gov't
Theophylline
spellingShingle (Rat hepatocyte)
Cytochrome P-450
Enzyme induction
Ferrochelatase
Glucose
δ-Aminolevulinate synthase
5 aminolevulinate synthase
adrenalin
bucladesine
cycloheximide
cytochrome p450
ferrochelatase
glucose
insulin
lead
phenobarbital
dose response
dose time effect relation
drug response
in vitro study
liver
liver cell
nonhuman
priority journal
5-Aminolevulinate Synthetase
Animal
Bucladesine
Cells, Cultured
Cycloheximide
Cytochrome P-450 Enzyme System
Enzyme Induction
Epinephrine
Ferrochelatase
Glucose
Hemeproteins
Insulin
Lead
Liver
Lyases
Male
Phenobarbital
Rats
Subcellular Fractions
Support, Non-U.S. Gov't
Theophylline
Cánepa, Eduardo Tomás
Llambías, Elena Blanca Cecilia
Grinstein, Moisés
Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead
topic_facet (Rat hepatocyte)
Cytochrome P-450
Enzyme induction
Ferrochelatase
Glucose
δ-Aminolevulinate synthase
5 aminolevulinate synthase
adrenalin
bucladesine
cycloheximide
cytochrome p450
ferrochelatase
glucose
insulin
lead
phenobarbital
dose response
dose time effect relation
drug response
in vitro study
liver
liver cell
nonhuman
priority journal
5-Aminolevulinate Synthetase
Animal
Bucladesine
Cells, Cultured
Cycloheximide
Cytochrome P-450 Enzyme System
Enzyme Induction
Epinephrine
Ferrochelatase
Glucose
Hemeproteins
Insulin
Lead
Liver
Lyases
Male
Phenobarbital
Rats
Subcellular Fractions
Support, Non-U.S. Gov't
Theophylline
description In the present work we have been able to demonstrate the phenobarbital and lead exert an inducing effect on the biosynthesis of δ-aminovulenic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes of normal adult rats. Dibutyryl cyclic AMP enhances the induction effect produced by phenobarbital in this in vitro system. Glucose inhibits the induction of δ-aminolevulinic acid synthase and ferrochelatase. This repression effect can be reversed with increasing concentrations of dibutyryl cyclic AMP. No glucose effect was observed on the phenobarbital- and lead-mediated inductions of cytochrome P-450. The present results add more experimental evidence to support the concept that the last enzyme of the heme pathway is inducible, and as such may have a significant role in regulatory mechanisms of porphyrin and heme biosynthesis. © 1985.
author Cánepa, Eduardo Tomás
Llambías, Elena Blanca Cecilia
Grinstein, Moisés
author_facet Cánepa, Eduardo Tomás
Llambías, Elena Blanca Cecilia
Grinstein, Moisés
author_sort Cánepa, Eduardo Tomás
title Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead
title_short Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead
title_full Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead
title_fullStr Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead
title_full_unstemmed Effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome P-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead
title_sort effect of glucose on induction of δ-aminolevulinic acid synthase, ferrochelatase and cytochrome p-450 hemoproteins in isolated rat hepatocytes by phenobarbital and lead
publishDate 1985
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v841_n2_p186_Canepa
http://hdl.handle.net/20.500.12110/paper_03044165_v841_n2_p186_Canepa
work_keys_str_mv AT canepaeduardotomas effectofglucoseoninductionofdaminolevulinicacidsynthaseferrochelataseandcytochromep450hemoproteinsinisolatedrathepatocytesbyphenobarbitalandlead
AT llambiaselenablancacecilia effectofglucoseoninductionofdaminolevulinicacidsynthaseferrochelataseandcytochromep450hemoproteinsinisolatedrathepatocytesbyphenobarbitalandlead
AT grinsteinmoises effectofglucoseoninductionofdaminolevulinicacidsynthaseferrochelataseandcytochromep450hemoproteinsinisolatedrathepatocytesbyphenobarbitalandlead
_version_ 1768542886870122496

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