Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation
1. 1. A non-sedimentable fraction of potato tuber has been found to catalyze [14C] glucose transfer from [14C] glucose 1-phosphate to an endogenous proteic acceptor in the absence of added primer. This transfer is activated by Mn2+. 2. 2. The labeled glucosylated product formed is trichloroacetic ac...
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1975
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v399_n2_p345_Tandecarz http://hdl.handle.net/20.500.12110/paper_03044165_v399_n2_p345_Tandecarz |
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paper:paper_03044165_v399_n2_p345_Tandecarz2023-06-08T15:29:55Z Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation Tandecarz, Juana Sara Lavintman, Nelly adenosine diphosphate glucose 1 phosphate glucosyltransferase glycoprotein guanosine diphosphate starch uridine diphosphate glucose c 14 in vitro study plant theoretical study Adenosine Diphosphate Glucose Glucosephosphates Glucosyltransferases Guanosine Diphosphate Sugars Kinetics Manganese Plant Proteins Plants Starch Uridine Diphosphate Glucose 1. 1. A non-sedimentable fraction of potato tuber has been found to catalyze [14C] glucose transfer from [14C] glucose 1-phosphate to an endogenous proteic acceptor in the absence of added primer. This transfer is activated by Mn2+. 2. 2. The labeled glucosylated product formed is trichloroacetic acid insoluble and sensitive to proteolytic and amylolytic digestions. It appears to be a glucoprotein with glucosyl chains bound to the peptide portion of the molecule through an unknown linkage. 3. 3. The carbohydrate portion of the glucoprotein can be released by prolonged incubations with the enzymatic preparation, and becomes in turn, trichloroacetic acid soluble and alcohol precipitable. 4. 4. Both products, the glucoprotein as well as the α-1,4-glucan that seems to arise from the enzymatic cleavage of the former, can be used as primers by the transglucosylating system with ADP[14C] glucose, UDP[14C] glucose or GDP[14C] glucose as glucosyl donors. The results presented in this paper are the first demonstration of soluble glucosyl transferases with the same glucose donor specificity to that of the particulate starch synthetase. 5. 5. This report presents further evidence in favor of the assumption of a glucoproteic intermediate in α-1,4-glucan synthesis initiation. © 1975. Fil:Tandecarz, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lavintman, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1975 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v399_n2_p345_Tandecarz http://hdl.handle.net/20.500.12110/paper_03044165_v399_n2_p345_Tandecarz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adenosine diphosphate glucose 1 phosphate glucosyltransferase glycoprotein guanosine diphosphate starch uridine diphosphate glucose c 14 in vitro study plant theoretical study Adenosine Diphosphate Glucose Glucosephosphates Glucosyltransferases Guanosine Diphosphate Sugars Kinetics Manganese Plant Proteins Plants Starch Uridine Diphosphate Glucose |
| spellingShingle |
adenosine diphosphate glucose 1 phosphate glucosyltransferase glycoprotein guanosine diphosphate starch uridine diphosphate glucose c 14 in vitro study plant theoretical study Adenosine Diphosphate Glucose Glucosephosphates Glucosyltransferases Guanosine Diphosphate Sugars Kinetics Manganese Plant Proteins Plants Starch Uridine Diphosphate Glucose Tandecarz, Juana Sara Lavintman, Nelly Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation |
| topic_facet |
adenosine diphosphate glucose 1 phosphate glucosyltransferase glycoprotein guanosine diphosphate starch uridine diphosphate glucose c 14 in vitro study plant theoretical study Adenosine Diphosphate Glucose Glucosephosphates Glucosyltransferases Guanosine Diphosphate Sugars Kinetics Manganese Plant Proteins Plants Starch Uridine Diphosphate Glucose |
| description |
1. 1. A non-sedimentable fraction of potato tuber has been found to catalyze [14C] glucose transfer from [14C] glucose 1-phosphate to an endogenous proteic acceptor in the absence of added primer. This transfer is activated by Mn2+. 2. 2. The labeled glucosylated product formed is trichloroacetic acid insoluble and sensitive to proteolytic and amylolytic digestions. It appears to be a glucoprotein with glucosyl chains bound to the peptide portion of the molecule through an unknown linkage. 3. 3. The carbohydrate portion of the glucoprotein can be released by prolonged incubations with the enzymatic preparation, and becomes in turn, trichloroacetic acid soluble and alcohol precipitable. 4. 4. Both products, the glucoprotein as well as the α-1,4-glucan that seems to arise from the enzymatic cleavage of the former, can be used as primers by the transglucosylating system with ADP[14C] glucose, UDP[14C] glucose or GDP[14C] glucose as glucosyl donors. The results presented in this paper are the first demonstration of soluble glucosyl transferases with the same glucose donor specificity to that of the particulate starch synthetase. 5. 5. This report presents further evidence in favor of the assumption of a glucoproteic intermediate in α-1,4-glucan synthesis initiation. © 1975. |
| author |
Tandecarz, Juana Sara Lavintman, Nelly |
| author_facet |
Tandecarz, Juana Sara Lavintman, Nelly |
| author_sort |
Tandecarz, Juana Sara |
| title |
Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation |
| title_short |
Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation |
| title_full |
Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation |
| title_fullStr |
Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation |
| title_full_unstemmed |
Biosynthesis of starch. Formation of a glucoproteic acceptor by a potato non-sedimentable preparation |
| title_sort |
biosynthesis of starch. formation of a glucoproteic acceptor by a potato non-sedimentable preparation |
| publishDate |
1975 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v399_n2_p345_Tandecarz http://hdl.handle.net/20.500.12110/paper_03044165_v399_n2_p345_Tandecarz |
| work_keys_str_mv |
AT tandecarzjuanasara biosynthesisofstarchformationofaglucoproteicacceptorbyapotatononsedimentablepreparation AT lavintmannelly biosynthesisofstarchformationofaglucoproteicacceptorbyapotatononsedimentablepreparation |
| _version_ |
1768544409751650304 |