Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase

1. 1. Further evidence for the formation of pyrrolic intermediates resulting from action of soybean callus porphobilinogenase on porphobilinogen in the absence or presence of ammonia, under anaerobic conditions, is reported. 2. 2. It was found that the activity of porphobilinogenase was affected by...

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Autores principales:	Stella de Rosellini, Ana María Cristina, Parera, Victoria Estela, Llambías, Elena Blanca Cecilia, Batlle, Alcira María del Carmen
Publicado:	1971
Materias:	ammonia ammonium sulfate carbon hydrolase isomerase lyase oxygen porphyrin propionic acid derivative pyrrole derivative anaerobic growth article enzymology gel chromatography kinetics plant stereoisomerism synthesis thin layer chromatography Aminohydrolases Ammonia Ammonium Sulfate Anaerobiosis Carbon Isotopes Chromatography, Gel Chromatography, Thin Layer Isomerases Kinetics Lyases Oxygen Plants Porphyrins Propionates Pyrroles Stereoisomerism
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v252_n3_p481_Stella http://hdl.handle.net/20.500.12110/paper_03044165_v252_n3_p481_Stella
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03044165_v252_n3_p481_Stella
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spelling	paper:paper_03044165_v252_n3_p481_Stella2023-06-08T15:29:55Z Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase Stella de Rosellini, Ana María Cristina Parera, Victoria Estela Llambías, Elena Blanca Cecilia Batlle, Alcira María del Carmen ammonia ammonium sulfate carbon hydrolase isomerase lyase oxygen porphyrin propionic acid derivative pyrrole derivative anaerobic growth article enzymology gel chromatography kinetics plant stereoisomerism synthesis thin layer chromatography Aminohydrolases Ammonia Ammonium Sulfate Anaerobiosis Carbon Isotopes Chromatography, Gel Chromatography, Thin Layer Isomerases Kinetics Lyases Oxygen Plants Porphyrins Propionates Pyrroles Stereoisomerism 1. 1. Further evidence for the formation of pyrrolic intermediates resulting from action of soybean callus porphobilinogenase on porphobilinogen in the absence or presence of ammonia, under anaerobic conditions, is reported. 2. 2. It was found that the activity of porphobilinogenase was affected by the presence of air; the yield of uroporphyrinogens was very low, although consumption of substrate was not greatly modified. These results suggested that some intermediate in the reaction was still being formed, but that this compound must be in the reduced form to be further converted into uroporphyrinogens. It was observed that ammonia probably produced as accumulation of pyrryl intermediates, but that they synthesize uroporphyrinogen III at a slower rate. 3. 3. Attempts were made to detect the formation of an enzyme-substrate complex, but they all failed. © 1971. Fil:Stella, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Parera, V.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llambías, E.B.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1971 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v252_n3_p481_Stella http://hdl.handle.net/20.500.12110/paper_03044165_v252_n3_p481_Stella
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ammonia ammonium sulfate carbon hydrolase isomerase lyase oxygen porphyrin propionic acid derivative pyrrole derivative anaerobic growth article enzymology gel chromatography kinetics plant stereoisomerism synthesis thin layer chromatography Aminohydrolases Ammonia Ammonium Sulfate Anaerobiosis Carbon Isotopes Chromatography, Gel Chromatography, Thin Layer Isomerases Kinetics Lyases Oxygen Plants Porphyrins Propionates Pyrroles Stereoisomerism
spellingShingle	ammonia ammonium sulfate carbon hydrolase isomerase lyase oxygen porphyrin propionic acid derivative pyrrole derivative anaerobic growth article enzymology gel chromatography kinetics plant stereoisomerism synthesis thin layer chromatography Aminohydrolases Ammonia Ammonium Sulfate Anaerobiosis Carbon Isotopes Chromatography, Gel Chromatography, Thin Layer Isomerases Kinetics Lyases Oxygen Plants Porphyrins Propionates Pyrroles Stereoisomerism Stella de Rosellini, Ana María Cristina Parera, Victoria Estela Llambías, Elena Blanca Cecilia Batlle, Alcira María del Carmen Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase
topic_facet	ammonia ammonium sulfate carbon hydrolase isomerase lyase oxygen porphyrin propionic acid derivative pyrrole derivative anaerobic growth article enzymology gel chromatography kinetics plant stereoisomerism synthesis thin layer chromatography Aminohydrolases Ammonia Ammonium Sulfate Anaerobiosis Carbon Isotopes Chromatography, Gel Chromatography, Thin Layer Isomerases Kinetics Lyases Oxygen Plants Porphyrins Propionates Pyrroles Stereoisomerism
description	1. 1. Further evidence for the formation of pyrrolic intermediates resulting from action of soybean callus porphobilinogenase on porphobilinogen in the absence or presence of ammonia, under anaerobic conditions, is reported. 2. 2. It was found that the activity of porphobilinogenase was affected by the presence of air; the yield of uroporphyrinogens was very low, although consumption of substrate was not greatly modified. These results suggested that some intermediate in the reaction was still being formed, but that this compound must be in the reduced form to be further converted into uroporphyrinogens. It was observed that ammonia probably produced as accumulation of pyrryl intermediates, but that they synthesize uroporphyrinogen III at a slower rate. 3. 3. Attempts were made to detect the formation of an enzyme-substrate complex, but they all failed. © 1971.
author	Stella de Rosellini, Ana María Cristina Parera, Victoria Estela Llambías, Elena Blanca Cecilia Batlle, Alcira María del Carmen
author_facet	Stella de Rosellini, Ana María Cristina Parera, Victoria Estela Llambías, Elena Blanca Cecilia Batlle, Alcira María del Carmen
author_sort	Stella de Rosellini, Ana María Cristina
title	Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase
title_short	Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase
title_full	Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase
title_fullStr	Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase
title_full_unstemmed	Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase
title_sort	pyrrylmethane intermediates in the synthesis of uroporphirigonen iii by soybean callus porphobilinogenase
publishDate	1971
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v252_n3_p481_Stella http://hdl.handle.net/20.500.12110/paper_03044165_v252_n3_p481_Stella
work_keys_str_mv	AT stelladerosellinianamariacristina pyrrylmethaneintermediatesinthesynthesisofuroporphirigoneniiibysoybeancallusporphobilinogenase AT pareravictoriaestela pyrrylmethaneintermediatesinthesynthesisofuroporphirigoneniiibysoybeancallusporphobilinogenase AT llambiaselenablancacecilia pyrrylmethaneintermediatesinthesynthesisofuroporphirigoneniiibysoybeancallusporphobilinogenase AT batllealciramariadelcarmen pyrrylmethaneintermediatesinthesynthesisofuroporphirigoneniiibysoybeancallusporphobilinogenase
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Pyrrylmethane intermediates in the synthesis of uroporphirigonen III by soybean callus porphobilinogenase

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