Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms

Background: Amylase is synthesized in submandibular glands (SMG) and released into the oral cavity to degrade carbohydrates in the mouth. Bitter taste receptors (T2R) belong to the G-protein coupled receptor (GPCR) family and are expressed in the taste cells and also in the digestive tract. Methods:...

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Autores principales:	Pagotto, Romina María del Luján, Pignataro, Omar Pedro
Publicado:	2011
Materias:	Amylase Bitter agonist cAMP Gi protein Inositol monophosphate Protein kinase A amylase cyclic AMP cyclic AMP dependent protein kinase cycloheximide denatonium benzoate guanine nucleotide binding protein gustducin inhibitory guanine nucleotide binding protein inositol phosphate phospholipase C beta2 theophylline unclassified drug animal tissue article bitter taste controlled study enzyme activity enzyme inhibition enzyme linked immunosorbent assay enzyme localization enzyme release immunohistochemistry mouse nonhuman priority journal protein expression protein function quantitative analysis radioimmunoassay signal transduction submandibular gland taste bud Western blotting Amylases Animals Blotting, Western Cyclic AMP Immunohistochemistry Mice Mice, Inbred BALB C Signal Transduction Submandibular Gland Murinae
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v1810_n12_p1212_Dasso http://hdl.handle.net/20.500.12110/paper_03044165_v1810_n12_p1212_Dasso
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_03044165_v1810_n12_p1212_Dasso2023-06-08T15:29:51Z Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms Pagotto, Romina María del Luján Pignataro, Omar Pedro Amylase Bitter agonist cAMP Gi protein Inositol monophosphate Protein kinase A amylase cyclic AMP cyclic AMP dependent protein kinase cycloheximide denatonium benzoate guanine nucleotide binding protein gustducin inhibitory guanine nucleotide binding protein inositol phosphate phospholipase C beta2 theophylline unclassified drug animal tissue article bitter taste controlled study enzyme activity enzyme inhibition enzyme linked immunosorbent assay enzyme localization enzyme release immunohistochemistry mouse nonhuman priority journal protein expression protein function quantitative analysis radioimmunoassay signal transduction submandibular gland taste bud Western blotting Amylases Animals Blotting, Western Cyclic AMP Immunohistochemistry Mice Mice, Inbred BALB C Signal Transduction Submandibular Gland Murinae Background: Amylase is synthesized in submandibular glands (SMG) and released into the oral cavity to degrade carbohydrates in the mouth. Bitter taste receptors (T2R) belong to the G-protein coupled receptor (GPCR) family and are expressed in the taste cells and also in the digestive tract. Methods: The activity of amylase secreted by murine SMG was measured, detecting maltose by Bernfeld's method. Amylase and T2R6 were detected by imunohistochemistry and Western blot. The expression of Ggustducin, Gi, and phospholipase Cβ2 was also studied by Western blot. cAMP levels were measured by radioimmunoassay and inositol monophosphate production was quantified by ELISA. Results: Theophylline, denatonium and cycloheximide exerted a dose-dependent inhibition on amylase secretion. This effect was reverted by preincubating SMG with an anti-Gαi antibody. cAMP production was increased by the same compounds, an effect that was also abrogated by an anti-Gαi antibody. Bitter compounds reduced inositol monophosphate formation in SMG and H-89, a protein kinase A inhibitor, reverted this action, revealing that this protein kinase down regulates phospholipase C activity. General significance: We demonstrated that theophylline, denatonium and cycloheximide inhibit salivary amylase secretion, activating an intracellular signaling pathway that involves cAMP and phospholipase C, that cross talks via protein kinase A. © 2011 Elsevier B.V. Fil:Pagotto, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pignataro, O.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v1810_n12_p1212_Dasso http://hdl.handle.net/20.500.12110/paper_03044165_v1810_n12_p1212_Dasso
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Amylase Bitter agonist cAMP Gi protein Inositol monophosphate Protein kinase A amylase cyclic AMP cyclic AMP dependent protein kinase cycloheximide denatonium benzoate guanine nucleotide binding protein gustducin inhibitory guanine nucleotide binding protein inositol phosphate phospholipase C beta2 theophylline unclassified drug animal tissue article bitter taste controlled study enzyme activity enzyme inhibition enzyme linked immunosorbent assay enzyme localization enzyme release immunohistochemistry mouse nonhuman priority journal protein expression protein function quantitative analysis radioimmunoassay signal transduction submandibular gland taste bud Western blotting Amylases Animals Blotting, Western Cyclic AMP Immunohistochemistry Mice Mice, Inbred BALB C Signal Transduction Submandibular Gland Murinae
spellingShingle	Amylase Bitter agonist cAMP Gi protein Inositol monophosphate Protein kinase A amylase cyclic AMP cyclic AMP dependent protein kinase cycloheximide denatonium benzoate guanine nucleotide binding protein gustducin inhibitory guanine nucleotide binding protein inositol phosphate phospholipase C beta2 theophylline unclassified drug animal tissue article bitter taste controlled study enzyme activity enzyme inhibition enzyme linked immunosorbent assay enzyme localization enzyme release immunohistochemistry mouse nonhuman priority journal protein expression protein function quantitative analysis radioimmunoassay signal transduction submandibular gland taste bud Western blotting Amylases Animals Blotting, Western Cyclic AMP Immunohistochemistry Mice Mice, Inbred BALB C Signal Transduction Submandibular Gland Murinae Pagotto, Romina María del Luján Pignataro, Omar Pedro Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms
topic_facet	Amylase Bitter agonist cAMP Gi protein Inositol monophosphate Protein kinase A amylase cyclic AMP cyclic AMP dependent protein kinase cycloheximide denatonium benzoate guanine nucleotide binding protein gustducin inhibitory guanine nucleotide binding protein inositol phosphate phospholipase C beta2 theophylline unclassified drug animal tissue article bitter taste controlled study enzyme activity enzyme inhibition enzyme linked immunosorbent assay enzyme localization enzyme release immunohistochemistry mouse nonhuman priority journal protein expression protein function quantitative analysis radioimmunoassay signal transduction submandibular gland taste bud Western blotting Amylases Animals Blotting, Western Cyclic AMP Immunohistochemistry Mice Mice, Inbred BALB C Signal Transduction Submandibular Gland Murinae
description	Background: Amylase is synthesized in submandibular glands (SMG) and released into the oral cavity to degrade carbohydrates in the mouth. Bitter taste receptors (T2R) belong to the G-protein coupled receptor (GPCR) family and are expressed in the taste cells and also in the digestive tract. Methods: The activity of amylase secreted by murine SMG was measured, detecting maltose by Bernfeld's method. Amylase and T2R6 were detected by imunohistochemistry and Western blot. The expression of Ggustducin, Gi, and phospholipase Cβ2 was also studied by Western blot. cAMP levels were measured by radioimmunoassay and inositol monophosphate production was quantified by ELISA. Results: Theophylline, denatonium and cycloheximide exerted a dose-dependent inhibition on amylase secretion. This effect was reverted by preincubating SMG with an anti-Gαi antibody. cAMP production was increased by the same compounds, an effect that was also abrogated by an anti-Gαi antibody. Bitter compounds reduced inositol monophosphate formation in SMG and H-89, a protein kinase A inhibitor, reverted this action, revealing that this protein kinase down regulates phospholipase C activity. General significance: We demonstrated that theophylline, denatonium and cycloheximide inhibit salivary amylase secretion, activating an intracellular signaling pathway that involves cAMP and phospholipase C, that cross talks via protein kinase A. © 2011 Elsevier B.V.
author	Pagotto, Romina María del Luján Pignataro, Omar Pedro
author_facet	Pagotto, Romina María del Luján Pignataro, Omar Pedro
author_sort	Pagotto, Romina María del Luján
title	Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms
title_short	Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms
title_full	Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms
title_fullStr	Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms
title_full_unstemmed	Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms
title_sort	effect of bitter compounds on amylase secretion in murine submandibular glands: signaling pathway mechanisms
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v1810_n12_p1212_Dasso http://hdl.handle.net/20.500.12110/paper_03044165_v1810_n12_p1212_Dasso
work_keys_str_mv	AT pagottorominamariadellujan effectofbittercompoundsonamylasesecretioninmurinesubmandibularglandssignalingpathwaymechanisms AT pignataroomarpedro effectofbittercompoundsonamylasesecretioninmurinesubmandibularglandssignalingpathwaymechanisms
_version_	1768546443844386816

Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms

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