Glutathione depletion and anaesthesia in mice alter heme and drug metabolising enzymes

The effects of enflurane and isoflurane on heme metabolism, its regulation, and on some parameters involved in the hepatic drug metabolising system in animals under GSH depletion were investigated. A single dose of the anaesthethics (1 ml kg-1, i.p.) was administered to control and GSH depleted mice...

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Autores principales:	Buzaleh, Ana María, Batlle, Alcira María del Carmen
Publicado:	2005
Materias:	Drug metabolising enzyme system Enflurane GSH Heme metabolism Isoflurane 5 aminolevulinate synthase drug metabolizing enzyme enflurane glutathione heme isoflurane anesthesia animal experiment animal model animal tissue article controlled study drug metabolism heme synthesis liver metabolism male metabolic disorder mouse nonhuman priority journal regulatory mechanism 5-Aminolevulinate Synthetase Acetaminophen Anesthesia, Inhalation Animals Glutathione Glutathione Transferase Heme Male Mice Pharmaceutical Preparations Animalia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v1723_n1-3_p128_Buzaleh http://hdl.handle.net/20.500.12110/paper_03044165_v1723_n1-3_p128_Buzaleh
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03044165_v1723_n1-3_p128_Buzaleh
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spelling	paper:paper_03044165_v1723_n1-3_p128_Buzaleh2023-06-08T15:29:51Z Glutathione depletion and anaesthesia in mice alter heme and drug metabolising enzymes Buzaleh, Ana María Batlle, Alcira María del Carmen Drug metabolising enzyme system Enflurane GSH Heme metabolism Isoflurane 5 aminolevulinate synthase drug metabolizing enzyme enflurane glutathione heme isoflurane anesthesia animal experiment animal model animal tissue article controlled study drug metabolism heme synthesis liver metabolism male metabolic disorder mouse nonhuman priority journal regulatory mechanism 5-Aminolevulinate Synthetase Acetaminophen Anesthesia, Inhalation Animals Enflurane Glutathione Glutathione Transferase Heme Isoflurane Male Mice Pharmaceutical Preparations Animalia The effects of enflurane and isoflurane on heme metabolism, its regulation, and on some parameters involved in the hepatic drug metabolising system in animals under GSH depletion were investigated. A single dose of the anaesthethics (1 ml kg-1, i.p.) was administered to control and GSH depleted mice, animals were sacrificed 20 min after. As a consequence of GSH depletion, a significant inhibition in δ-Aminolevulinic acid synthetase activity, the first enzyme of heme bioxynthesis, and a striking induction in Heme oxygenase activity, the main enzyme of heme metabolism, were observed. Cytochrome P-450 levels and the activities of P-4502E1 and glutathione S-transferase were increased. These changes in heme metabolism and drug metabolising enzyme system were not altered further by the administration of enflurane or isoflurane. These findings would indicate that the status of oxidative stress produced by GSH depletion could not be affected by these anaesthetics and/or that disturbances in heme metabolism were already too important to undergo further variations. © 2005 Elsevier B.V. All rights reserved. Fil:Buzaleh, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A.M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v1723_n1-3_p128_Buzaleh http://hdl.handle.net/20.500.12110/paper_03044165_v1723_n1-3_p128_Buzaleh
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Drug metabolising enzyme system Enflurane GSH Heme metabolism Isoflurane 5 aminolevulinate synthase drug metabolizing enzyme enflurane glutathione heme isoflurane anesthesia animal experiment animal model animal tissue article controlled study drug metabolism heme synthesis liver metabolism male metabolic disorder mouse nonhuman priority journal regulatory mechanism 5-Aminolevulinate Synthetase Acetaminophen Anesthesia, Inhalation Animals Enflurane Glutathione Glutathione Transferase Heme Isoflurane Male Mice Pharmaceutical Preparations Animalia
spellingShingle	Drug metabolising enzyme system Enflurane GSH Heme metabolism Isoflurane 5 aminolevulinate synthase drug metabolizing enzyme enflurane glutathione heme isoflurane anesthesia animal experiment animal model animal tissue article controlled study drug metabolism heme synthesis liver metabolism male metabolic disorder mouse nonhuman priority journal regulatory mechanism 5-Aminolevulinate Synthetase Acetaminophen Anesthesia, Inhalation Animals Enflurane Glutathione Glutathione Transferase Heme Isoflurane Male Mice Pharmaceutical Preparations Animalia Buzaleh, Ana María Batlle, Alcira María del Carmen Glutathione depletion and anaesthesia in mice alter heme and drug metabolising enzymes
topic_facet	Drug metabolising enzyme system Enflurane GSH Heme metabolism Isoflurane 5 aminolevulinate synthase drug metabolizing enzyme enflurane glutathione heme isoflurane anesthesia animal experiment animal model animal tissue article controlled study drug metabolism heme synthesis liver metabolism male metabolic disorder mouse nonhuman priority journal regulatory mechanism 5-Aminolevulinate Synthetase Acetaminophen Anesthesia, Inhalation Animals Enflurane Glutathione Glutathione Transferase Heme Isoflurane Male Mice Pharmaceutical Preparations Animalia
description	The effects of enflurane and isoflurane on heme metabolism, its regulation, and on some parameters involved in the hepatic drug metabolising system in animals under GSH depletion were investigated. A single dose of the anaesthethics (1 ml kg-1, i.p.) was administered to control and GSH depleted mice, animals were sacrificed 20 min after. As a consequence of GSH depletion, a significant inhibition in δ-Aminolevulinic acid synthetase activity, the first enzyme of heme bioxynthesis, and a striking induction in Heme oxygenase activity, the main enzyme of heme metabolism, were observed. Cytochrome P-450 levels and the activities of P-4502E1 and glutathione S-transferase were increased. These changes in heme metabolism and drug metabolising enzyme system were not altered further by the administration of enflurane or isoflurane. These findings would indicate that the status of oxidative stress produced by GSH depletion could not be affected by these anaesthetics and/or that disturbances in heme metabolism were already too important to undergo further variations. © 2005 Elsevier B.V. All rights reserved.
author	Buzaleh, Ana María Batlle, Alcira María del Carmen
author_facet	Buzaleh, Ana María Batlle, Alcira María del Carmen
author_sort	Buzaleh, Ana María
title	Glutathione depletion and anaesthesia in mice alter heme and drug metabolising enzymes
title_short	Glutathione depletion and anaesthesia in mice alter heme and drug metabolising enzymes
title_full	Glutathione depletion and anaesthesia in mice alter heme and drug metabolising enzymes
title_fullStr	Glutathione depletion and anaesthesia in mice alter heme and drug metabolising enzymes
title_full_unstemmed	Glutathione depletion and anaesthesia in mice alter heme and drug metabolising enzymes
title_sort	glutathione depletion and anaesthesia in mice alter heme and drug metabolising enzymes
publishDate	2005
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044165_v1723_n1-3_p128_Buzaleh http://hdl.handle.net/20.500.12110/paper_03044165_v1723_n1-3_p128_Buzaleh
work_keys_str_mv	AT buzalehanamaria glutathionedepletionandanaesthesiainmicealterhemeanddrugmetabolisingenzymes AT batllealciramariadelcarmen glutathionedepletionandanaesthesiainmicealterhemeanddrugmetabolisingenzymes
_version_	1768541988456497152

Glutathione depletion and anaesthesia in mice alter heme and drug metabolising enzymes

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