In vivo activity of albendazole in combination with thymol against Echinococcus multilocularis
Human alveolar echinococcosis (AE) is caused by the fox tapeworm Echinococcus multilocularis and is usually lethal if left untreated. The current strategy for treating human AE is surgical resection of the parasite mass complemented by chemotherapy with benzimidazole compounds. However, reliable che...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044017_v212_n3-4_p193_Albani http://hdl.handle.net/20.500.12110/paper_03044017_v212_n3-4_p193_Albani |
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paper:paper_03044017_v212_n3-4_p193_Albani2025-07-30T18:07:22Z In vivo activity of albendazole in combination with thymol against Echinococcus multilocularis Albendazole Alveolar echinococcosis Echinococcus multilocularis Liver enzymes Thymol alanine aminotransferase albendazole alkaline phosphatase aspartate aminotransferase thymol albendazole anthelmintic agent thymol animal cell animal experiment animal model animal tissue Article autopsy cell ultrastructure cell viability controlled study drug efficacy echinococcosis Echinococcus multilocularis enzyme activity enzyme assay female in vivo study metacestode microscopy mouse nonhuman peritoneal cavity scanning electron microscopy transmission electron microscopy animal combination drug therapy echinococcosis pathology ultrastructure Albendazole Animals Anthelmintics Drug Therapy, Combination Echinococcosis Echinococcus multilocularis Female Mice Thymol Human alveolar echinococcosis (AE) is caused by the fox tapeworm Echinococcus multilocularis and is usually lethal if left untreated. The current strategy for treating human AE is surgical resection of the parasite mass complemented by chemotherapy with benzimidazole compounds. However, reliable chemotherapeutic alternatives have not yet been developed stimulating the research of new treatment strategies such as the use of medicinal plants. The aim of the current study was to investigate the efficacy of the combination albendazole (ABZ) + thymol on mice infected with E. multilocularis metacestodes. For this purpose, mice infected with parasite material were treated daily for 20 days with ABZ (5 mg/kg), thymol (40 mg/kg) or ABZ (5 mg/kg) + thymol (40 mg/kg) or left untreated as controls. After mice were euthanized, cysts were removed from the peritoneal cavity and the treatment efficacy was evaluated by the mean cysts weight, viability of protoscoleces and ultrastructural changes of cysts and protoscoleces. The application of thymol or the combination of ABZ + thymol resulted in a significant reduction of the cysts weight compared to untreated mice. We also found that although ABZ and thymol had a scolicidal effect, the combination of the two compounds had a considerably stronger effect showing a reduction in the protoscoleces viability of 62%. These results were also corroborated by optical microscopy, SEM and TEM. Protoscoleces recovered from ABZ or thymol treated mice showed alterations as contraction of the soma region, rostellar disorganization and presence of blebs in the tegument. However both drugs when combined lead to a total loss of the typical morphology of protoscoleces. All cysts removed from control mice appeared intact and no change in ultrastructure was detected. In contrast, cysts developed in mice treated with ABZ revealed changes in the germinal layer as reduction in cell number, while the treatment with thymol or the ABZ + thymol combination predominantly showed presence of cell debris. On the other hand, no differences were found in alkaline phosphatase (AP), glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities between control and treated mice, indicating the lack of toxicity of the different drug treatments during the experiment. Because combined ABZ + thymol treatment exhibited higher treatment efficiency compared with the drugs applied separately against murine experimental alveolar echinococcosis, we propose it would be a useful option for the treatment of human AE. © 2015 Elsevier B.V. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044017_v212_n3-4_p193_Albani http://hdl.handle.net/20.500.12110/paper_03044017_v212_n3-4_p193_Albani |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Albendazole Alveolar echinococcosis Echinococcus multilocularis Liver enzymes Thymol alanine aminotransferase albendazole alkaline phosphatase aspartate aminotransferase thymol albendazole anthelmintic agent thymol animal cell animal experiment animal model animal tissue Article autopsy cell ultrastructure cell viability controlled study drug efficacy echinococcosis Echinococcus multilocularis enzyme activity enzyme assay female in vivo study metacestode microscopy mouse nonhuman peritoneal cavity scanning electron microscopy transmission electron microscopy animal combination drug therapy echinococcosis pathology ultrastructure Albendazole Animals Anthelmintics Drug Therapy, Combination Echinococcosis Echinococcus multilocularis Female Mice Thymol |
| spellingShingle |
Albendazole Alveolar echinococcosis Echinococcus multilocularis Liver enzymes Thymol alanine aminotransferase albendazole alkaline phosphatase aspartate aminotransferase thymol albendazole anthelmintic agent thymol animal cell animal experiment animal model animal tissue Article autopsy cell ultrastructure cell viability controlled study drug efficacy echinococcosis Echinococcus multilocularis enzyme activity enzyme assay female in vivo study metacestode microscopy mouse nonhuman peritoneal cavity scanning electron microscopy transmission electron microscopy animal combination drug therapy echinococcosis pathology ultrastructure Albendazole Animals Anthelmintics Drug Therapy, Combination Echinococcosis Echinococcus multilocularis Female Mice Thymol In vivo activity of albendazole in combination with thymol against Echinococcus multilocularis |
| topic_facet |
Albendazole Alveolar echinococcosis Echinococcus multilocularis Liver enzymes Thymol alanine aminotransferase albendazole alkaline phosphatase aspartate aminotransferase thymol albendazole anthelmintic agent thymol animal cell animal experiment animal model animal tissue Article autopsy cell ultrastructure cell viability controlled study drug efficacy echinococcosis Echinococcus multilocularis enzyme activity enzyme assay female in vivo study metacestode microscopy mouse nonhuman peritoneal cavity scanning electron microscopy transmission electron microscopy animal combination drug therapy echinococcosis pathology ultrastructure Albendazole Animals Anthelmintics Drug Therapy, Combination Echinococcosis Echinococcus multilocularis Female Mice Thymol |
| description |
Human alveolar echinococcosis (AE) is caused by the fox tapeworm Echinococcus multilocularis and is usually lethal if left untreated. The current strategy for treating human AE is surgical resection of the parasite mass complemented by chemotherapy with benzimidazole compounds. However, reliable chemotherapeutic alternatives have not yet been developed stimulating the research of new treatment strategies such as the use of medicinal plants. The aim of the current study was to investigate the efficacy of the combination albendazole (ABZ) + thymol on mice infected with E. multilocularis metacestodes. For this purpose, mice infected with parasite material were treated daily for 20 days with ABZ (5 mg/kg), thymol (40 mg/kg) or ABZ (5 mg/kg) + thymol (40 mg/kg) or left untreated as controls. After mice were euthanized, cysts were removed from the peritoneal cavity and the treatment efficacy was evaluated by the mean cysts weight, viability of protoscoleces and ultrastructural changes of cysts and protoscoleces. The application of thymol or the combination of ABZ + thymol resulted in a significant reduction of the cysts weight compared to untreated mice. We also found that although ABZ and thymol had a scolicidal effect, the combination of the two compounds had a considerably stronger effect showing a reduction in the protoscoleces viability of 62%. These results were also corroborated by optical microscopy, SEM and TEM. Protoscoleces recovered from ABZ or thymol treated mice showed alterations as contraction of the soma region, rostellar disorganization and presence of blebs in the tegument. However both drugs when combined lead to a total loss of the typical morphology of protoscoleces. All cysts removed from control mice appeared intact and no change in ultrastructure was detected. In contrast, cysts developed in mice treated with ABZ revealed changes in the germinal layer as reduction in cell number, while the treatment with thymol or the ABZ + thymol combination predominantly showed presence of cell debris. On the other hand, no differences were found in alkaline phosphatase (AP), glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities between control and treated mice, indicating the lack of toxicity of the different drug treatments during the experiment. Because combined ABZ + thymol treatment exhibited higher treatment efficiency compared with the drugs applied separately against murine experimental alveolar echinococcosis, we propose it would be a useful option for the treatment of human AE. © 2015 Elsevier B.V. |
| title |
In vivo activity of albendazole in combination with thymol against Echinococcus multilocularis |
| title_short |
In vivo activity of albendazole in combination with thymol against Echinococcus multilocularis |
| title_full |
In vivo activity of albendazole in combination with thymol against Echinococcus multilocularis |
| title_fullStr |
In vivo activity of albendazole in combination with thymol against Echinococcus multilocularis |
| title_full_unstemmed |
In vivo activity of albendazole in combination with thymol against Echinococcus multilocularis |
| title_sort |
in vivo activity of albendazole in combination with thymol against echinococcus multilocularis |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03044017_v212_n3-4_p193_Albani http://hdl.handle.net/20.500.12110/paper_03044017_v212_n3-4_p193_Albani |
| _version_ |
1840323465969664000 |