Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats
Mineralocorticoids (MC) play an important role in development of salt appetite. Part of this effect involves the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, in which MC treatment increases arginine vasopressin (AVP) synthesis and release. Since the AVP system is also modulated by...
| Publicado: |
2002
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v329_n3_p344_Saravia http://hdl.handle.net/20.500.12110/paper_03043940_v329_n3_p344_Saravia |
| Aporte de: |
| id |
paper:paper_03043940_v329_n3_p344_Saravia |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_03043940_v329_n3_p344_Saravia2023-06-08T15:29:31Z Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats Mineralocorticoids Nicotinamide dinucleotide diphoshate-diaphorase Nitric oxide synthase Salt appetite argipressin cytochrome b5 reductase deoxycorticosterone acetate messenger RNA nicotinamide adenine dinucleotide phosphate nitric oxide nitric oxide synthase sodium chloride animal cell animal experiment animal tissue anterior hypothalamus appetite brain nerve cell cell count controlled study drug effect enzyme activation enzyme activity food intake hypothalamus nucleus immunocompetent cell immunoreactivity male nonhuman priority journal rat review supraoptic nucleus Mineralocorticoids (MC) play an important role in development of salt appetite. Part of this effect involves the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, in which MC treatment increases arginine vasopressin (AVP) synthesis and release. Since the AVP system is also modulated by nitric oxide (NO), we studied if deoxycorticosterone acetate (DOCA) treatment changed the number of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) active neurons and neuronal NO synthase (nNOS)-immunoreactive (IR) cells in the PVN and SON. After four injections of DOCA (10 mg/rat per day), rats developed a salt appetite and increased NADPH-d active and nNOS-IR neurons in both nuclei. A single DOCA injection did not change salt consumption or nNOS-IR cells, but increased the number of NADPH-d positive neurons in the PVN only. Therefore, while acute MC treatment stimulated the activity of pre-existing enzyme, chronic steroid treatment recruited additional neurons showing nNOS immunoreactivity/NADPH-d activity. These data suggest a role for NO produced in the PVN and SON in DOCA stimulatory effects on AVP mRNA and salt appetite. © 2002 Published by Elsevier Science Ireland Ltd. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v329_n3_p344_Saravia http://hdl.handle.net/20.500.12110/paper_03043940_v329_n3_p344_Saravia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Mineralocorticoids Nicotinamide dinucleotide diphoshate-diaphorase Nitric oxide synthase Salt appetite argipressin cytochrome b5 reductase deoxycorticosterone acetate messenger RNA nicotinamide adenine dinucleotide phosphate nitric oxide nitric oxide synthase sodium chloride animal cell animal experiment animal tissue anterior hypothalamus appetite brain nerve cell cell count controlled study drug effect enzyme activation enzyme activity food intake hypothalamus nucleus immunocompetent cell immunoreactivity male nonhuman priority journal rat review supraoptic nucleus |
| spellingShingle |
Mineralocorticoids Nicotinamide dinucleotide diphoshate-diaphorase Nitric oxide synthase Salt appetite argipressin cytochrome b5 reductase deoxycorticosterone acetate messenger RNA nicotinamide adenine dinucleotide phosphate nitric oxide nitric oxide synthase sodium chloride animal cell animal experiment animal tissue anterior hypothalamus appetite brain nerve cell cell count controlled study drug effect enzyme activation enzyme activity food intake hypothalamus nucleus immunocompetent cell immunoreactivity male nonhuman priority journal rat review supraoptic nucleus Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats |
| topic_facet |
Mineralocorticoids Nicotinamide dinucleotide diphoshate-diaphorase Nitric oxide synthase Salt appetite argipressin cytochrome b5 reductase deoxycorticosterone acetate messenger RNA nicotinamide adenine dinucleotide phosphate nitric oxide nitric oxide synthase sodium chloride animal cell animal experiment animal tissue anterior hypothalamus appetite brain nerve cell cell count controlled study drug effect enzyme activation enzyme activity food intake hypothalamus nucleus immunocompetent cell immunoreactivity male nonhuman priority journal rat review supraoptic nucleus |
| description |
Mineralocorticoids (MC) play an important role in development of salt appetite. Part of this effect involves the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei, in which MC treatment increases arginine vasopressin (AVP) synthesis and release. Since the AVP system is also modulated by nitric oxide (NO), we studied if deoxycorticosterone acetate (DOCA) treatment changed the number of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) active neurons and neuronal NO synthase (nNOS)-immunoreactive (IR) cells in the PVN and SON. After four injections of DOCA (10 mg/rat per day), rats developed a salt appetite and increased NADPH-d active and nNOS-IR neurons in both nuclei. A single DOCA injection did not change salt consumption or nNOS-IR cells, but increased the number of NADPH-d positive neurons in the PVN only. Therefore, while acute MC treatment stimulated the activity of pre-existing enzyme, chronic steroid treatment recruited additional neurons showing nNOS immunoreactivity/NADPH-d activity. These data suggest a role for NO produced in the PVN and SON in DOCA stimulatory effects on AVP mRNA and salt appetite. © 2002 Published by Elsevier Science Ireland Ltd. |
| title |
Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats |
| title_short |
Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats |
| title_full |
Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats |
| title_fullStr |
Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats |
| title_full_unstemmed |
Deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats |
| title_sort |
deoxycorticosterone stimulates the activity of nicotinamide adenine dinucleotide phosphate-diaphorase/nitric oxide synthase immunoreactivity in hypothalamic nuclei of rats |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03043940_v329_n3_p344_Saravia http://hdl.handle.net/20.500.12110/paper_03043940_v329_n3_p344_Saravia |
| _version_ |
1768543993828737024 |