VIP induces the decidualization program and conditions the immunoregulation of the implantation process
The decidualization process involves phenotype and functional changes on endometrial cells and the modulation of mediators with immunoregulatory properties as the vasoactive intestinal peptide (VIP). We investigate VIP contribution to the decidualization program and to immunoregulation throughout th...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v460_n_p63_Grasso http://hdl.handle.net/20.500.12110/paper_03037207_v460_n_p63_Grasso |
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paper:paper_03037207_v460_n_p63_Grasso2023-06-08T15:29:13Z VIP induces the decidualization program and conditions the immunoregulation of the implantation process Decidualization Immunoregulation Implantation VIP cysteine rich protein 61 dibutyrylcytidine 3',5' phosphate interleukin 8 medroxyprogesterone membrane protein monocyte chemotactic protein 1 prolactin protein KLF13 stromal cell derived factor 1 unclassified drug vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor biological marker vasoactive intestinal polypeptide Article blastocyst CD4+ T lymphocyte controlled study decidualization dendritic cell human human cell immunoregulation in vitro study nidation priority journal protein expression regulatory T lymphocyte stromal cell line tumor spheroid biological model cell line conditioned medium cytology decidua drug effect endometrium female immunological tolerance immunology metabolism nidation pharmacology stroma cell trophoblast tumor microenvironment Biomarkers Blastocyst Cell Line Cellular Microenvironment Culture Media, Conditioned Decidua Embryo Implantation Endometrium Female Humans Immune Tolerance Models, Biological Stromal Cells Trophoblasts Vasoactive Intestinal Peptide The decidualization process involves phenotype and functional changes on endometrial cells and the modulation of mediators with immunoregulatory properties as the vasoactive intestinal peptide (VIP). We investigate VIP contribution to the decidualization program and to immunoregulation throughout the human embryo implantation process. The decidualization of Human endometrial stromal cell line (HESC) with Medroxyprogesterone-dibutyryl-cAMP increased VIP/VPAC-receptors system. In fact, VIP could induce decidualization increasing differentiation markers (IGFBP1, PRL, KLF13/KLF9 ratio, CXCL12, CXCL8 and CCL2) and allowing Blastocyst-like spheroids (BLS) invasion in an in vitro model of embryo implantation. Focus on the tolerogenic effects, decidualized cells induced a semi-mature profile on maternal dendritic cells; restrained CD4+ cells recruitment while increased regulatory T-cells recruitment. Interestingly, the human blastocyst conditioned media from developmentally impaired embryos diminished the invasion and T-regulatory cells recruitment in these settings. These evidences suggest that VIP contributes to the implantation process inducing decidualization, allowing BLS invasion and favoring a tolerogenic micro-environment. © 2017 Elsevier B.V. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v460_n_p63_Grasso http://hdl.handle.net/20.500.12110/paper_03037207_v460_n_p63_Grasso |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Decidualization Immunoregulation Implantation VIP cysteine rich protein 61 dibutyrylcytidine 3',5' phosphate interleukin 8 medroxyprogesterone membrane protein monocyte chemotactic protein 1 prolactin protein KLF13 stromal cell derived factor 1 unclassified drug vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor biological marker vasoactive intestinal polypeptide Article blastocyst CD4+ T lymphocyte controlled study decidualization dendritic cell human human cell immunoregulation in vitro study nidation priority journal protein expression regulatory T lymphocyte stromal cell line tumor spheroid biological model cell line conditioned medium cytology decidua drug effect endometrium female immunological tolerance immunology metabolism nidation pharmacology stroma cell trophoblast tumor microenvironment Biomarkers Blastocyst Cell Line Cellular Microenvironment Culture Media, Conditioned Decidua Embryo Implantation Endometrium Female Humans Immune Tolerance Models, Biological Stromal Cells Trophoblasts Vasoactive Intestinal Peptide |
| spellingShingle |
Decidualization Immunoregulation Implantation VIP cysteine rich protein 61 dibutyrylcytidine 3',5' phosphate interleukin 8 medroxyprogesterone membrane protein monocyte chemotactic protein 1 prolactin protein KLF13 stromal cell derived factor 1 unclassified drug vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor biological marker vasoactive intestinal polypeptide Article blastocyst CD4+ T lymphocyte controlled study decidualization dendritic cell human human cell immunoregulation in vitro study nidation priority journal protein expression regulatory T lymphocyte stromal cell line tumor spheroid biological model cell line conditioned medium cytology decidua drug effect endometrium female immunological tolerance immunology metabolism nidation pharmacology stroma cell trophoblast tumor microenvironment Biomarkers Blastocyst Cell Line Cellular Microenvironment Culture Media, Conditioned Decidua Embryo Implantation Endometrium Female Humans Immune Tolerance Models, Biological Stromal Cells Trophoblasts Vasoactive Intestinal Peptide VIP induces the decidualization program and conditions the immunoregulation of the implantation process |
| topic_facet |
Decidualization Immunoregulation Implantation VIP cysteine rich protein 61 dibutyrylcytidine 3',5' phosphate interleukin 8 medroxyprogesterone membrane protein monocyte chemotactic protein 1 prolactin protein KLF13 stromal cell derived factor 1 unclassified drug vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor biological marker vasoactive intestinal polypeptide Article blastocyst CD4+ T lymphocyte controlled study decidualization dendritic cell human human cell immunoregulation in vitro study nidation priority journal protein expression regulatory T lymphocyte stromal cell line tumor spheroid biological model cell line conditioned medium cytology decidua drug effect endometrium female immunological tolerance immunology metabolism nidation pharmacology stroma cell trophoblast tumor microenvironment Biomarkers Blastocyst Cell Line Cellular Microenvironment Culture Media, Conditioned Decidua Embryo Implantation Endometrium Female Humans Immune Tolerance Models, Biological Stromal Cells Trophoblasts Vasoactive Intestinal Peptide |
| description |
The decidualization process involves phenotype and functional changes on endometrial cells and the modulation of mediators with immunoregulatory properties as the vasoactive intestinal peptide (VIP). We investigate VIP contribution to the decidualization program and to immunoregulation throughout the human embryo implantation process. The decidualization of Human endometrial stromal cell line (HESC) with Medroxyprogesterone-dibutyryl-cAMP increased VIP/VPAC-receptors system. In fact, VIP could induce decidualization increasing differentiation markers (IGFBP1, PRL, KLF13/KLF9 ratio, CXCL12, CXCL8 and CCL2) and allowing Blastocyst-like spheroids (BLS) invasion in an in vitro model of embryo implantation. Focus on the tolerogenic effects, decidualized cells induced a semi-mature profile on maternal dendritic cells; restrained CD4+ cells recruitment while increased regulatory T-cells recruitment. Interestingly, the human blastocyst conditioned media from developmentally impaired embryos diminished the invasion and T-regulatory cells recruitment in these settings. These evidences suggest that VIP contributes to the implantation process inducing decidualization, allowing BLS invasion and favoring a tolerogenic micro-environment. © 2017 Elsevier B.V. |
| title |
VIP induces the decidualization program and conditions the immunoregulation of the implantation process |
| title_short |
VIP induces the decidualization program and conditions the immunoregulation of the implantation process |
| title_full |
VIP induces the decidualization program and conditions the immunoregulation of the implantation process |
| title_fullStr |
VIP induces the decidualization program and conditions the immunoregulation of the implantation process |
| title_full_unstemmed |
VIP induces the decidualization program and conditions the immunoregulation of the implantation process |
| title_sort |
vip induces the decidualization program and conditions the immunoregulation of the implantation process |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v460_n_p63_Grasso http://hdl.handle.net/20.500.12110/paper_03037207_v460_n_p63_Grasso |
| _version_ |
1768545093664374784 |