Effect of prolactin on the steroidogenic response of rat luteal cells
The role of prolactin on some ovarian functions was studied in collagenase-dispersed luteal cells obtained from PMSG/hCG-primed rats. The in vitro effect of ovine prolactin (oPrl) on luteal cell function was assayed. This hormone produced a dose-dependent increase of progesterone production and an a...
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1984
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v36_n3_p187_Tesone http://hdl.handle.net/20.500.12110/paper_03037207_v36_n3_p187_Tesone |
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paper:paper_03037207_v36_n3_p187_Tesone2023-06-08T15:29:10Z Effect of prolactin on the steroidogenic response of rat luteal cells LH receptors Luteal cells progesterone and cAMP production prolactin chorionic gonadotropin progesterone prolactin radioisotope seric gonadotropin animal cell animal experiment corpus luteum dose response drug administration drug binding drug blood level drug comparison drug efficacy drug interaction drug response endocrine system etiology female genital system luteinizing hormone i 125 methodology nonhuman ovary progesterone h 3 rat steroidogenesis therapy Animal Bromocriptine Chorionic Gonadotropin Corpus Luteum Cyclic AMP Female Gonadotropins, Equine Organ Weight Progesterone Prolactin Rats Receptors, Cell Surface Receptors, LH Sulpiride Support, Non-U.S. Gov't The role of prolactin on some ovarian functions was studied in collagenase-dispersed luteal cells obtained from PMSG/hCG-primed rats. The in vitro effect of ovine prolactin (oPrl) on luteal cell function was assayed. This hormone produced a dose-dependent increase of progesterone production and an additive effect on hCG stimulation. oPrl had no effect on cAMP production. Chronic effects of prolactin were studied in sulpiride (S), bromocriptine (Br) and oPrl-treated rats. Serum levels of prolactin were significantly higher in S-treated animals whereas Br administration rendered undetectable values. Serum progesterone was reduced in Br-treated animals and LH levels were similar in all groups studied. In vitro studies demonstrated a marked reduction of hCG stimulation of progesterone and cAMP production by luteal cells from hypoprolactinemic animals, while a significant increase was observed in hyperprolactinemic states. oPrl and S treatment significantly increased ovarian LH binding sites while a reduction was observed in Br-treated rats. These data suggest that luteal cell function is regulated by circulating levels of prolactin and that this hormone has some direct effect on the steroidogenic process. © 1984. 1984 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v36_n3_p187_Tesone http://hdl.handle.net/20.500.12110/paper_03037207_v36_n3_p187_Tesone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
LH receptors Luteal cells progesterone and cAMP production prolactin chorionic gonadotropin progesterone prolactin radioisotope seric gonadotropin animal cell animal experiment corpus luteum dose response drug administration drug binding drug blood level drug comparison drug efficacy drug interaction drug response endocrine system etiology female genital system luteinizing hormone i 125 methodology nonhuman ovary progesterone h 3 rat steroidogenesis therapy Animal Bromocriptine Chorionic Gonadotropin Corpus Luteum Cyclic AMP Female Gonadotropins, Equine Organ Weight Progesterone Prolactin Rats Receptors, Cell Surface Receptors, LH Sulpiride Support, Non-U.S. Gov't |
| spellingShingle |
LH receptors Luteal cells progesterone and cAMP production prolactin chorionic gonadotropin progesterone prolactin radioisotope seric gonadotropin animal cell animal experiment corpus luteum dose response drug administration drug binding drug blood level drug comparison drug efficacy drug interaction drug response endocrine system etiology female genital system luteinizing hormone i 125 methodology nonhuman ovary progesterone h 3 rat steroidogenesis therapy Animal Bromocriptine Chorionic Gonadotropin Corpus Luteum Cyclic AMP Female Gonadotropins, Equine Organ Weight Progesterone Prolactin Rats Receptors, Cell Surface Receptors, LH Sulpiride Support, Non-U.S. Gov't Effect of prolactin on the steroidogenic response of rat luteal cells |
| topic_facet |
LH receptors Luteal cells progesterone and cAMP production prolactin chorionic gonadotropin progesterone prolactin radioisotope seric gonadotropin animal cell animal experiment corpus luteum dose response drug administration drug binding drug blood level drug comparison drug efficacy drug interaction drug response endocrine system etiology female genital system luteinizing hormone i 125 methodology nonhuman ovary progesterone h 3 rat steroidogenesis therapy Animal Bromocriptine Chorionic Gonadotropin Corpus Luteum Cyclic AMP Female Gonadotropins, Equine Organ Weight Progesterone Prolactin Rats Receptors, Cell Surface Receptors, LH Sulpiride Support, Non-U.S. Gov't |
| description |
The role of prolactin on some ovarian functions was studied in collagenase-dispersed luteal cells obtained from PMSG/hCG-primed rats. The in vitro effect of ovine prolactin (oPrl) on luteal cell function was assayed. This hormone produced a dose-dependent increase of progesterone production and an additive effect on hCG stimulation. oPrl had no effect on cAMP production. Chronic effects of prolactin were studied in sulpiride (S), bromocriptine (Br) and oPrl-treated rats. Serum levels of prolactin were significantly higher in S-treated animals whereas Br administration rendered undetectable values. Serum progesterone was reduced in Br-treated animals and LH levels were similar in all groups studied. In vitro studies demonstrated a marked reduction of hCG stimulation of progesterone and cAMP production by luteal cells from hypoprolactinemic animals, while a significant increase was observed in hyperprolactinemic states. oPrl and S treatment significantly increased ovarian LH binding sites while a reduction was observed in Br-treated rats. These data suggest that luteal cell function is regulated by circulating levels of prolactin and that this hormone has some direct effect on the steroidogenic process. © 1984. |
| title |
Effect of prolactin on the steroidogenic response of rat luteal cells |
| title_short |
Effect of prolactin on the steroidogenic response of rat luteal cells |
| title_full |
Effect of prolactin on the steroidogenic response of rat luteal cells |
| title_fullStr |
Effect of prolactin on the steroidogenic response of rat luteal cells |
| title_full_unstemmed |
Effect of prolactin on the steroidogenic response of rat luteal cells |
| title_sort |
effect of prolactin on the steroidogenic response of rat luteal cells |
| publishDate |
1984 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v36_n3_p187_Tesone http://hdl.handle.net/20.500.12110/paper_03037207_v36_n3_p187_Tesone |
| _version_ |
1768542840736972800 |