Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells

Experimental evidence has demonstrated that multiple doses of LH will increase the steroidogenic capacity of Leydig cells. This work was undertaken with the aim of defining the effect of a second hCG administration on the desensitized state, measuring binding of the gonadotropin and the steroidogeni...

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Autores principales:	Calvo, Juan Carlos, Radicella, Juan Pablo, Pignataro, Omar Pedro, Charreau, Eduardo Hernán
Publicado:	1984
Materias:	desensitization hCG testis bucladesine chorionic gonadotropin cycloheximide radioisotope testosterone animal cell animal experiment chorionic gonadotropin i 125 drug efficacy drug receptor binding endocrine system hydroxyprogesterone h 3 intramuscular drug administration leydig cell male genital system nonhuman pharmacokinetics progesterone h 3 rat steroidogenesis testosterone h 3 testosterone release Animal Bucladesine Chorionic Gonadotropin Cyclic AMP Drug Resistance Hydroxyprogesterones Leydig Cells Male Progesterone Rats Rats, Inbred Strains Support, Non-U.S. Gov't Testosterone
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v34_n1_p31_Calvo http://hdl.handle.net/20.500.12110/paper_03037207_v34_n1_p31_Calvo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_03037207_v34_n1_p31_Calvo2023-06-08T15:29:09Z Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells Calvo, Juan Carlos Radicella, Juan Pablo Pignataro, Omar Pedro Charreau, Eduardo Hernán desensitization hCG testis bucladesine chorionic gonadotropin cycloheximide radioisotope testosterone animal cell animal experiment chorionic gonadotropin i 125 desensitization drug efficacy drug receptor binding endocrine system hydroxyprogesterone h 3 intramuscular drug administration leydig cell male genital system nonhuman pharmacokinetics progesterone h 3 rat steroidogenesis testis testosterone h 3 testosterone release Animal Bucladesine Chorionic Gonadotropin Cyclic AMP Drug Resistance Hydroxyprogesterones Leydig Cells Male Progesterone Rats Rats, Inbred Strains Support, Non-U.S. Gov't Testosterone Experimental evidence has demonstrated that multiple doses of LH will increase the steroidogenic capacity of Leydig cells. This work was undertaken with the aim of defining the effect of a second hCG administration on the desensitized state, measuring binding of the gonadotropin and the steroidogenic capacity of rat testes. A single injection of 200 IU hCG induced a sharp increase of plasma testosterone which was still evident 24 h later. A second peak was observed at 72 h. The in vivo refractoriness of Leydig cells between 24 and 72 h after the single injection was proved by the fact that a second administration of hCG, 2 h before sacrifice, did not induce any increase in plasma testosterone. A second administration of 200 IU hCG, 48 h after the first injection, showed a similar pattern but on the 5th day there was an increased stimulation of testosterone production with respect to that obtained after a single dose of hCG. The in vitro studies on testicular binding capacity and steroidogenic responsiveness showed that the second administration of hCG, 48 h after the first injection, maintained the testicular binding capacity at the lowest level and the 'adenylate cyclase desensitization' but restored the steroidogenic capacity to even supramaximal values, compared to normal rats, 3 days after this second hCG administration. These results would support a dissociation between receptor loss and maximal testosterone synthesis as well as possibly indicating an alternative pathway different from the classical. © 1984. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Radicella, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pignataro, O.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1984 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v34_n1_p31_Calvo http://hdl.handle.net/20.500.12110/paper_03037207_v34_n1_p31_Calvo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	desensitization hCG testis bucladesine chorionic gonadotropin cycloheximide radioisotope testosterone animal cell animal experiment chorionic gonadotropin i 125 desensitization drug efficacy drug receptor binding endocrine system hydroxyprogesterone h 3 intramuscular drug administration leydig cell male genital system nonhuman pharmacokinetics progesterone h 3 rat steroidogenesis testis testosterone h 3 testosterone release Animal Bucladesine Chorionic Gonadotropin Cyclic AMP Drug Resistance Hydroxyprogesterones Leydig Cells Male Progesterone Rats Rats, Inbred Strains Support, Non-U.S. Gov't Testosterone
spellingShingle	desensitization hCG testis bucladesine chorionic gonadotropin cycloheximide radioisotope testosterone animal cell animal experiment chorionic gonadotropin i 125 desensitization drug efficacy drug receptor binding endocrine system hydroxyprogesterone h 3 intramuscular drug administration leydig cell male genital system nonhuman pharmacokinetics progesterone h 3 rat steroidogenesis testis testosterone h 3 testosterone release Animal Bucladesine Chorionic Gonadotropin Cyclic AMP Drug Resistance Hydroxyprogesterones Leydig Cells Male Progesterone Rats Rats, Inbred Strains Support, Non-U.S. Gov't Testosterone Calvo, Juan Carlos Radicella, Juan Pablo Pignataro, Omar Pedro Charreau, Eduardo Hernán Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells
topic_facet	desensitization hCG testis bucladesine chorionic gonadotropin cycloheximide radioisotope testosterone animal cell animal experiment chorionic gonadotropin i 125 desensitization drug efficacy drug receptor binding endocrine system hydroxyprogesterone h 3 intramuscular drug administration leydig cell male genital system nonhuman pharmacokinetics progesterone h 3 rat steroidogenesis testis testosterone h 3 testosterone release Animal Bucladesine Chorionic Gonadotropin Cyclic AMP Drug Resistance Hydroxyprogesterones Leydig Cells Male Progesterone Rats Rats, Inbred Strains Support, Non-U.S. Gov't Testosterone
description	Experimental evidence has demonstrated that multiple doses of LH will increase the steroidogenic capacity of Leydig cells. This work was undertaken with the aim of defining the effect of a second hCG administration on the desensitized state, measuring binding of the gonadotropin and the steroidogenic capacity of rat testes. A single injection of 200 IU hCG induced a sharp increase of plasma testosterone which was still evident 24 h later. A second peak was observed at 72 h. The in vivo refractoriness of Leydig cells between 24 and 72 h after the single injection was proved by the fact that a second administration of hCG, 2 h before sacrifice, did not induce any increase in plasma testosterone. A second administration of 200 IU hCG, 48 h after the first injection, showed a similar pattern but on the 5th day there was an increased stimulation of testosterone production with respect to that obtained after a single dose of hCG. The in vitro studies on testicular binding capacity and steroidogenic responsiveness showed that the second administration of hCG, 48 h after the first injection, maintained the testicular binding capacity at the lowest level and the 'adenylate cyclase desensitization' but restored the steroidogenic capacity to even supramaximal values, compared to normal rats, 3 days after this second hCG administration. These results would support a dissociation between receptor loss and maximal testosterone synthesis as well as possibly indicating an alternative pathway different from the classical. © 1984.
author	Calvo, Juan Carlos Radicella, Juan Pablo Pignataro, Omar Pedro Charreau, Eduardo Hernán
author_facet	Calvo, Juan Carlos Radicella, Juan Pablo Pignataro, Omar Pedro Charreau, Eduardo Hernán
author_sort	Calvo, Juan Carlos
title	Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells
title_short	Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells
title_full	Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells
title_fullStr	Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells
title_full_unstemmed	Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells
title_sort	effect of a second injection of human chorionic gonadotropin on the desensitized leydig cells
publishDate	1984
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v34_n1_p31_Calvo http://hdl.handle.net/20.500.12110/paper_03037207_v34_n1_p31_Calvo
work_keys_str_mv	AT calvojuancarlos effectofasecondinjectionofhumanchorionicgonadotropinonthedesensitizedleydigcells AT radicellajuanpablo effectofasecondinjectionofhumanchorionicgonadotropinonthedesensitizedleydigcells AT pignataroomarpedro effectofasecondinjectionofhumanchorionicgonadotropinonthedesensitizedleydigcells AT charreaueduardohernan effectofasecondinjectionofhumanchorionicgonadotropinonthedesensitizedleydigcells
_version_	1768545975074291712

Effect of a second injection of human chorionic gonadotropin on the desensitized Leydig cells

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