Angiopoietin 1 reduces rat follicular atresia mediated by apoptosis through the PI3K/Akt pathway
The aim of this study was to determine the effect of the local inhibition of ANGPT1 on steroid production, proliferation and apoptosis of ovarian follicular cells and on the PI3K/AKT pathway. We also examined the effect of ANGPTs on follicular cell apoptosis and proliferation in early antral follicl...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v343_n1-2_p79_Parborell http://hdl.handle.net/20.500.12110/paper_03037207_v343_n1-2_p79_Parborell |
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paper:paper_03037207_v343_n1-2_p79_Parborell |
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paper:paper_03037207_v343_n1-2_p79_Parborell2023-06-08T15:29:09Z Angiopoietin 1 reduces rat follicular atresia mediated by apoptosis through the PI3K/Akt pathway Angiogenesis Angiopoietins Apoptosis Follicular development Ovary actin androsterone angiopoietin 1 angiopoietin 2 antibody caspase 3 cycline diethylstilbestrol estradiol phosphatidylinositol 3 kinase protein kinase B animal cell animal experiment animal tissue antigen expression apoptosis article cell proliferation controlled study enzyme phosphorylation female nonhuman ovary follicle atresia ovary follicle cell prepuberty priority journal protein function rat steroidogenesis Angiopoietin-1 Animals Apoptosis Caspase 3 Cell Proliferation Cells, Cultured Female Follicular Atresia Humans Ovarian Follicle Phosphatidylinositol 3-Kinases Proto-Oncogene Proteins c-akt Rats Rats, Sprague-Dawley Signal Transduction Steroids The aim of this study was to determine the effect of the local inhibition of ANGPT1 on steroid production, proliferation and apoptosis of ovarian follicular cells and on the PI3K/AKT pathway. We also examined the effect of ANGPTs on follicular cell apoptosis and proliferation in early antral follicles (EAFs) in culture. Follicular cells expressing PCNA decreased after ANGPT1 Ab treatment. Moreover, ANGPT1 inhibition increased the levels of active caspase 3 and androsterone, but decreased estradiol, AKT phosphorylation and the area of smooth muscle cell actin. In cultured EAFs from prepubertal rats treated with diethylstilbestrol (DES), ANGPT1 increased PCNA and decreased apoptosis while ANGPT2 reversed these effects. These results show that ANGPT1 alters steroidogenesis, reduces ovarian apoptosis, and stimulates cell proliferation in antral follicles. ANGPT1 may exert these roles by regulating ovarian vascular stability and/or by a direct effect on follicular cells, possibly involving the PI3K/AKT pathway. © 2011 Elsevier Ireland Ltd. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v343_n1-2_p79_Parborell http://hdl.handle.net/20.500.12110/paper_03037207_v343_n1-2_p79_Parborell |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Angiogenesis Angiopoietins Apoptosis Follicular development Ovary actin androsterone angiopoietin 1 angiopoietin 2 antibody caspase 3 cycline diethylstilbestrol estradiol phosphatidylinositol 3 kinase protein kinase B animal cell animal experiment animal tissue antigen expression apoptosis article cell proliferation controlled study enzyme phosphorylation female nonhuman ovary follicle atresia ovary follicle cell prepuberty priority journal protein function rat steroidogenesis Angiopoietin-1 Animals Apoptosis Caspase 3 Cell Proliferation Cells, Cultured Female Follicular Atresia Humans Ovarian Follicle Phosphatidylinositol 3-Kinases Proto-Oncogene Proteins c-akt Rats Rats, Sprague-Dawley Signal Transduction Steroids |
| spellingShingle |
Angiogenesis Angiopoietins Apoptosis Follicular development Ovary actin androsterone angiopoietin 1 angiopoietin 2 antibody caspase 3 cycline diethylstilbestrol estradiol phosphatidylinositol 3 kinase protein kinase B animal cell animal experiment animal tissue antigen expression apoptosis article cell proliferation controlled study enzyme phosphorylation female nonhuman ovary follicle atresia ovary follicle cell prepuberty priority journal protein function rat steroidogenesis Angiopoietin-1 Animals Apoptosis Caspase 3 Cell Proliferation Cells, Cultured Female Follicular Atresia Humans Ovarian Follicle Phosphatidylinositol 3-Kinases Proto-Oncogene Proteins c-akt Rats Rats, Sprague-Dawley Signal Transduction Steroids Angiopoietin 1 reduces rat follicular atresia mediated by apoptosis through the PI3K/Akt pathway |
| topic_facet |
Angiogenesis Angiopoietins Apoptosis Follicular development Ovary actin androsterone angiopoietin 1 angiopoietin 2 antibody caspase 3 cycline diethylstilbestrol estradiol phosphatidylinositol 3 kinase protein kinase B animal cell animal experiment animal tissue antigen expression apoptosis article cell proliferation controlled study enzyme phosphorylation female nonhuman ovary follicle atresia ovary follicle cell prepuberty priority journal protein function rat steroidogenesis Angiopoietin-1 Animals Apoptosis Caspase 3 Cell Proliferation Cells, Cultured Female Follicular Atresia Humans Ovarian Follicle Phosphatidylinositol 3-Kinases Proto-Oncogene Proteins c-akt Rats Rats, Sprague-Dawley Signal Transduction Steroids |
| description |
The aim of this study was to determine the effect of the local inhibition of ANGPT1 on steroid production, proliferation and apoptosis of ovarian follicular cells and on the PI3K/AKT pathway. We also examined the effect of ANGPTs on follicular cell apoptosis and proliferation in early antral follicles (EAFs) in culture. Follicular cells expressing PCNA decreased after ANGPT1 Ab treatment. Moreover, ANGPT1 inhibition increased the levels of active caspase 3 and androsterone, but decreased estradiol, AKT phosphorylation and the area of smooth muscle cell actin. In cultured EAFs from prepubertal rats treated with diethylstilbestrol (DES), ANGPT1 increased PCNA and decreased apoptosis while ANGPT2 reversed these effects. These results show that ANGPT1 alters steroidogenesis, reduces ovarian apoptosis, and stimulates cell proliferation in antral follicles. ANGPT1 may exert these roles by regulating ovarian vascular stability and/or by a direct effect on follicular cells, possibly involving the PI3K/AKT pathway. © 2011 Elsevier Ireland Ltd. |
| title |
Angiopoietin 1 reduces rat follicular atresia mediated by apoptosis through the PI3K/Akt pathway |
| title_short |
Angiopoietin 1 reduces rat follicular atresia mediated by apoptosis through the PI3K/Akt pathway |
| title_full |
Angiopoietin 1 reduces rat follicular atresia mediated by apoptosis through the PI3K/Akt pathway |
| title_fullStr |
Angiopoietin 1 reduces rat follicular atresia mediated by apoptosis through the PI3K/Akt pathway |
| title_full_unstemmed |
Angiopoietin 1 reduces rat follicular atresia mediated by apoptosis through the PI3K/Akt pathway |
| title_sort |
angiopoietin 1 reduces rat follicular atresia mediated by apoptosis through the pi3k/akt pathway |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v343_n1-2_p79_Parborell http://hdl.handle.net/20.500.12110/paper_03037207_v343_n1-2_p79_Parborell |
| _version_ |
1768546629565022208 |