Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis

The subcellular location of some enzymes responsible for cholesterol biosynthesis was studied in metrizamide-purified rat Leydig cells.The highest activity of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase), a key regulatory enzyme in the cholesterol pathway, was associated with...

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Autores principales:	Pignataro, Omar Pedro, Radicella, Juan Pablo, Calvo, Juan Carlos, Charreau, Eduardo Hernán
Publicado:	1983
Materias:	cholesterol biosynthesis HMG-CoA reductase Leydig cell hydroxymethylglutaryl coenzyme a reductase radioisotope acetyl coenzyme a c 14 animal experiment biological model cholesterol synthesis endocrine system leydig cell male genital system mitochondrion nonhuman rat Acetates Acetyl Coenzyme A Animal Cell Compartmentation Cholesterol Hydroxymethylglutaryl CoA Reductases Leydig Cells Male Mitochondria Rats Support, Non-U.S. Gov't
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v33_n1_p53_Pignataro http://hdl.handle.net/20.500.12110/paper_03037207_v33_n1_p53_Pignataro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03037207_v33_n1_p53_Pignataro
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spelling	paper:paper_03037207_v33_n1_p53_Pignataro2025-07-30T18:06:50Z Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis Pignataro, Omar Pedro Radicella, Juan Pablo Calvo, Juan Carlos Charreau, Eduardo Hernán cholesterol biosynthesis HMG-CoA reductase Leydig cell hydroxymethylglutaryl coenzyme a reductase radioisotope acetyl coenzyme a c 14 animal experiment biological model cholesterol synthesis endocrine system leydig cell male genital system mitochondrion nonhuman rat Acetates Acetyl Coenzyme A Animal Cell Compartmentation Cholesterol Hydroxymethylglutaryl CoA Reductases Leydig Cells Male Mitochondria Rats Support, Non-U.S. Gov't The subcellular location of some enzymes responsible for cholesterol biosynthesis was studied in metrizamide-purified rat Leydig cells.The highest activity of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase), a key regulatory enzyme in the cholesterol pathway, was associated with highly enriched mitochondrial fractions with recovery of 62% of the total activity and was located on the inner membrane. A significant part of the activity (35%) was also present in the cytoplasm. The activity of this enzyme in the other subcellular fractions was negligible. The HMG-CoA synthase activity was also found almost entirely in the mitochondria (90%). Otherwise no detectable activity of HMG-CoA lyase was present in the subcellular fractions studied. Furthermore, cholesterol may be synthesized from acetyl-CoA inside the mitochondrion, since a significant incorporation (90%) of [14C]acetyl-CoA into digitonin-precipitable sterols was observed in this organelle and only 10% in the cytoplasmic fraction. The evidence strongly suggests that much of the cholesterol biosynthesis that takes place in Leydig cells is carried out within the mitochondria. © 1983. Fil:Pignataro, O.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Radicella, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1983 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v33_n1_p53_Pignataro http://hdl.handle.net/20.500.12110/paper_03037207_v33_n1_p53_Pignataro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cholesterol biosynthesis HMG-CoA reductase Leydig cell hydroxymethylglutaryl coenzyme a reductase radioisotope acetyl coenzyme a c 14 animal experiment biological model cholesterol synthesis endocrine system leydig cell male genital system mitochondrion nonhuman rat Acetates Acetyl Coenzyme A Animal Cell Compartmentation Cholesterol Hydroxymethylglutaryl CoA Reductases Leydig Cells Male Mitochondria Rats Support, Non-U.S. Gov't
spellingShingle	cholesterol biosynthesis HMG-CoA reductase Leydig cell hydroxymethylglutaryl coenzyme a reductase radioisotope acetyl coenzyme a c 14 animal experiment biological model cholesterol synthesis endocrine system leydig cell male genital system mitochondrion nonhuman rat Acetates Acetyl Coenzyme A Animal Cell Compartmentation Cholesterol Hydroxymethylglutaryl CoA Reductases Leydig Cells Male Mitochondria Rats Support, Non-U.S. Gov't Pignataro, Omar Pedro Radicella, Juan Pablo Calvo, Juan Carlos Charreau, Eduardo Hernán Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
topic_facet	cholesterol biosynthesis HMG-CoA reductase Leydig cell hydroxymethylglutaryl coenzyme a reductase radioisotope acetyl coenzyme a c 14 animal experiment biological model cholesterol synthesis endocrine system leydig cell male genital system mitochondrion nonhuman rat Acetates Acetyl Coenzyme A Animal Cell Compartmentation Cholesterol Hydroxymethylglutaryl CoA Reductases Leydig Cells Male Mitochondria Rats Support, Non-U.S. Gov't
description	The subcellular location of some enzymes responsible for cholesterol biosynthesis was studied in metrizamide-purified rat Leydig cells.The highest activity of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase), a key regulatory enzyme in the cholesterol pathway, was associated with highly enriched mitochondrial fractions with recovery of 62% of the total activity and was located on the inner membrane. A significant part of the activity (35%) was also present in the cytoplasm. The activity of this enzyme in the other subcellular fractions was negligible. The HMG-CoA synthase activity was also found almost entirely in the mitochondria (90%). Otherwise no detectable activity of HMG-CoA lyase was present in the subcellular fractions studied. Furthermore, cholesterol may be synthesized from acetyl-CoA inside the mitochondrion, since a significant incorporation (90%) of [14C]acetyl-CoA into digitonin-precipitable sterols was observed in this organelle and only 10% in the cytoplasmic fraction. The evidence strongly suggests that much of the cholesterol biosynthesis that takes place in Leydig cells is carried out within the mitochondria. © 1983.
author	Pignataro, Omar Pedro Radicella, Juan Pablo Calvo, Juan Carlos Charreau, Eduardo Hernán
author_facet	Pignataro, Omar Pedro Radicella, Juan Pablo Calvo, Juan Carlos Charreau, Eduardo Hernán
author_sort	Pignataro, Omar Pedro
title	Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
title_short	Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
title_full	Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
title_fullStr	Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
title_full_unstemmed	Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
title_sort	mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
publishDate	1983
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v33_n1_p53_Pignataro http://hdl.handle.net/20.500.12110/paper_03037207_v33_n1_p53_Pignataro
work_keys_str_mv	AT pignataroomarpedro mitochondrialbiosynthesisofcholesterolinleydigcellsfromrattestis AT radicellajuanpablo mitochondrialbiosynthesisofcholesterolinleydigcellsfromrattestis AT calvojuancarlos mitochondrialbiosynthesisofcholesterolinleydigcellsfromrattestis AT charreaueduardohernan mitochondrialbiosynthesisofcholesterolinleydigcellsfromrattestis
_version_	1840322585776095232

Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis

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