Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis
The subcellular location of some enzymes responsible for cholesterol biosynthesis was studied in metrizamide-purified rat Leydig cells.The highest activity of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase), a key regulatory enzyme in the cholesterol pathway, was associated with...
Guardado en:
Autores principales: | , , , |
---|---|
Publicado: |
1983
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v33_n1_p53_Pignataro http://hdl.handle.net/20.500.12110/paper_03037207_v33_n1_p53_Pignataro |
Aporte de: |
id |
paper:paper_03037207_v33_n1_p53_Pignataro |
---|---|
record_format |
dspace |
spelling |
paper:paper_03037207_v33_n1_p53_Pignataro2023-06-08T15:29:08Z Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis Pignataro, Omar Pedro Radicella, Juan Pablo Calvo, Juan Carlos Charreau, Eduardo Hernán cholesterol biosynthesis HMG-CoA reductase Leydig cell hydroxymethylglutaryl coenzyme a reductase radioisotope acetyl coenzyme a c 14 animal experiment biological model cholesterol synthesis endocrine system leydig cell male genital system mitochondrion nonhuman rat Acetates Acetyl Coenzyme A Animal Cell Compartmentation Cholesterol Hydroxymethylglutaryl CoA Reductases Leydig Cells Male Mitochondria Rats Support, Non-U.S. Gov't The subcellular location of some enzymes responsible for cholesterol biosynthesis was studied in metrizamide-purified rat Leydig cells.The highest activity of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase), a key regulatory enzyme in the cholesterol pathway, was associated with highly enriched mitochondrial fractions with recovery of 62% of the total activity and was located on the inner membrane. A significant part of the activity (35%) was also present in the cytoplasm. The activity of this enzyme in the other subcellular fractions was negligible. The HMG-CoA synthase activity was also found almost entirely in the mitochondria (90%). Otherwise no detectable activity of HMG-CoA lyase was present in the subcellular fractions studied. Furthermore, cholesterol may be synthesized from acetyl-CoA inside the mitochondrion, since a significant incorporation (90%) of [14C]acetyl-CoA into digitonin-precipitable sterols was observed in this organelle and only 10% in the cytoplasmic fraction. The evidence strongly suggests that much of the cholesterol biosynthesis that takes place in Leydig cells is carried out within the mitochondria. © 1983. Fil:Pignataro, O.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Radicella, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1983 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v33_n1_p53_Pignataro http://hdl.handle.net/20.500.12110/paper_03037207_v33_n1_p53_Pignataro |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
cholesterol biosynthesis HMG-CoA reductase Leydig cell hydroxymethylglutaryl coenzyme a reductase radioisotope acetyl coenzyme a c 14 animal experiment biological model cholesterol synthesis endocrine system leydig cell male genital system mitochondrion nonhuman rat Acetates Acetyl Coenzyme A Animal Cell Compartmentation Cholesterol Hydroxymethylglutaryl CoA Reductases Leydig Cells Male Mitochondria Rats Support, Non-U.S. Gov't |
spellingShingle |
cholesterol biosynthesis HMG-CoA reductase Leydig cell hydroxymethylglutaryl coenzyme a reductase radioisotope acetyl coenzyme a c 14 animal experiment biological model cholesterol synthesis endocrine system leydig cell male genital system mitochondrion nonhuman rat Acetates Acetyl Coenzyme A Animal Cell Compartmentation Cholesterol Hydroxymethylglutaryl CoA Reductases Leydig Cells Male Mitochondria Rats Support, Non-U.S. Gov't Pignataro, Omar Pedro Radicella, Juan Pablo Calvo, Juan Carlos Charreau, Eduardo Hernán Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis |
topic_facet |
cholesterol biosynthesis HMG-CoA reductase Leydig cell hydroxymethylglutaryl coenzyme a reductase radioisotope acetyl coenzyme a c 14 animal experiment biological model cholesterol synthesis endocrine system leydig cell male genital system mitochondrion nonhuman rat Acetates Acetyl Coenzyme A Animal Cell Compartmentation Cholesterol Hydroxymethylglutaryl CoA Reductases Leydig Cells Male Mitochondria Rats Support, Non-U.S. Gov't |
description |
The subcellular location of some enzymes responsible for cholesterol biosynthesis was studied in metrizamide-purified rat Leydig cells.The highest activity of 3-hydroxy-3-methyl glutaryl coenzyme A reductase (HMG-CoA reductase), a key regulatory enzyme in the cholesterol pathway, was associated with highly enriched mitochondrial fractions with recovery of 62% of the total activity and was located on the inner membrane. A significant part of the activity (35%) was also present in the cytoplasm. The activity of this enzyme in the other subcellular fractions was negligible. The HMG-CoA synthase activity was also found almost entirely in the mitochondria (90%). Otherwise no detectable activity of HMG-CoA lyase was present in the subcellular fractions studied. Furthermore, cholesterol may be synthesized from acetyl-CoA inside the mitochondrion, since a significant incorporation (90%) of [14C]acetyl-CoA into digitonin-precipitable sterols was observed in this organelle and only 10% in the cytoplasmic fraction. The evidence strongly suggests that much of the cholesterol biosynthesis that takes place in Leydig cells is carried out within the mitochondria. © 1983. |
author |
Pignataro, Omar Pedro Radicella, Juan Pablo Calvo, Juan Carlos Charreau, Eduardo Hernán |
author_facet |
Pignataro, Omar Pedro Radicella, Juan Pablo Calvo, Juan Carlos Charreau, Eduardo Hernán |
author_sort |
Pignataro, Omar Pedro |
title |
Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis |
title_short |
Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis |
title_full |
Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis |
title_fullStr |
Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis |
title_full_unstemmed |
Mitochondrial biosynthesis of cholesterol in leydig cells from rat testis |
title_sort |
mitochondrial biosynthesis of cholesterol in leydig cells from rat testis |
publishDate |
1983 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v33_n1_p53_Pignataro http://hdl.handle.net/20.500.12110/paper_03037207_v33_n1_p53_Pignataro |
work_keys_str_mv |
AT pignataroomarpedro mitochondrialbiosynthesisofcholesterolinleydigcellsfromrattestis AT radicellajuanpablo mitochondrialbiosynthesisofcholesterolinleydigcellsfromrattestis AT calvojuancarlos mitochondrialbiosynthesisofcholesterolinleydigcellsfromrattestis AT charreaueduardohernan mitochondrialbiosynthesisofcholesterolinleydigcellsfromrattestis |
_version_ |
1768542037015003136 |