Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells
The aim of the present work was to analyze the direct effect of VEGF in follicular cell proliferation, apoptosis and activation of the PI3K/AKT and ERK/MEK signaling pathways in early antral follicles or granulosa cells. Antral follicles or granulosa cells were isolated from prepubertal female Sprag...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v325_n1-2_p93_Irusta http://hdl.handle.net/20.500.12110/paper_03037207_v325_n1-2_p93_Irusta |
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paper:paper_03037207_v325_n1-2_p93_Irusta2023-06-08T15:29:06Z Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells Apoptosis Ovary Proliferation Signaling pathways VEGF diethylstilbestrol DNA mitogen activated protein kinase mitogen activated protein kinase 1 mitogen activated protein kinase 3 phosphatidylinositol 3 kinase protein kinase B vasculotropin animal cell animal cell culture apoptosis article cell isolation cell proliferation cell survival controlled study DNA fragmentation DNA isolation enzyme activation enzyme phosphorylation female granulosa cell nick end labeling nonhuman ovary follicle cell priority journal rat Western blotting Animals Apoptosis Cell Proliferation Cell Survival Cells, Cultured Enzyme Activation Extracellular Signal-Regulated MAP Kinases Female Granulosa Cells Oncogene Protein v-akt Ovarian Follicle Rats Rats, Sprague-Dawley Sexual Maturation Signal Transduction Vascular Endothelial Growth Factor A The aim of the present work was to analyze the direct effect of VEGF in follicular cell proliferation, apoptosis and activation of the PI3K/AKT and ERK/MEK signaling pathways in early antral follicles or granulosa cells. Antral follicles or granulosa cells were isolated from prepubertal female Sprague Dawley rats treated with DES.VEGF directly stimulates follicular cell proliferation and it also decreases apoptosis by inhibiting caspase 3 activation. In addition, VEGF increases the proliferation and inhibits the apoptosis of isolated granulosa cells in culture. VEGF activates the PI3K/AKT pathway evidenced by an increase in AKT phosphorylation levels and induces the phosphorylation of ERK1/2 in cultured antral follicles. These results demonstrate for the first time that VEGF has a proliferative and cytoprotective role in early antral follicles and in granulosa cells isolated from DES treated prepubertal rats and suggest that PI3K/AKT and ERK/MEK signaling pathways are involved in these processes. © 2010 Elsevier Ireland Ltd. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v325_n1-2_p93_Irusta http://hdl.handle.net/20.500.12110/paper_03037207_v325_n1-2_p93_Irusta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apoptosis Ovary Proliferation Signaling pathways VEGF diethylstilbestrol DNA mitogen activated protein kinase mitogen activated protein kinase 1 mitogen activated protein kinase 3 phosphatidylinositol 3 kinase protein kinase B vasculotropin animal cell animal cell culture apoptosis article cell isolation cell proliferation cell survival controlled study DNA fragmentation DNA isolation enzyme activation enzyme phosphorylation female granulosa cell nick end labeling nonhuman ovary follicle cell priority journal rat Western blotting Animals Apoptosis Cell Proliferation Cell Survival Cells, Cultured Enzyme Activation Extracellular Signal-Regulated MAP Kinases Female Granulosa Cells Oncogene Protein v-akt Ovarian Follicle Rats Rats, Sprague-Dawley Sexual Maturation Signal Transduction Vascular Endothelial Growth Factor A |
| spellingShingle |
Apoptosis Ovary Proliferation Signaling pathways VEGF diethylstilbestrol DNA mitogen activated protein kinase mitogen activated protein kinase 1 mitogen activated protein kinase 3 phosphatidylinositol 3 kinase protein kinase B vasculotropin animal cell animal cell culture apoptosis article cell isolation cell proliferation cell survival controlled study DNA fragmentation DNA isolation enzyme activation enzyme phosphorylation female granulosa cell nick end labeling nonhuman ovary follicle cell priority journal rat Western blotting Animals Apoptosis Cell Proliferation Cell Survival Cells, Cultured Enzyme Activation Extracellular Signal-Regulated MAP Kinases Female Granulosa Cells Oncogene Protein v-akt Ovarian Follicle Rats Rats, Sprague-Dawley Sexual Maturation Signal Transduction Vascular Endothelial Growth Factor A Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells |
| topic_facet |
Apoptosis Ovary Proliferation Signaling pathways VEGF diethylstilbestrol DNA mitogen activated protein kinase mitogen activated protein kinase 1 mitogen activated protein kinase 3 phosphatidylinositol 3 kinase protein kinase B vasculotropin animal cell animal cell culture apoptosis article cell isolation cell proliferation cell survival controlled study DNA fragmentation DNA isolation enzyme activation enzyme phosphorylation female granulosa cell nick end labeling nonhuman ovary follicle cell priority journal rat Western blotting Animals Apoptosis Cell Proliferation Cell Survival Cells, Cultured Enzyme Activation Extracellular Signal-Regulated MAP Kinases Female Granulosa Cells Oncogene Protein v-akt Ovarian Follicle Rats Rats, Sprague-Dawley Sexual Maturation Signal Transduction Vascular Endothelial Growth Factor A |
| description |
The aim of the present work was to analyze the direct effect of VEGF in follicular cell proliferation, apoptosis and activation of the PI3K/AKT and ERK/MEK signaling pathways in early antral follicles or granulosa cells. Antral follicles or granulosa cells were isolated from prepubertal female Sprague Dawley rats treated with DES.VEGF directly stimulates follicular cell proliferation and it also decreases apoptosis by inhibiting caspase 3 activation. In addition, VEGF increases the proliferation and inhibits the apoptosis of isolated granulosa cells in culture. VEGF activates the PI3K/AKT pathway evidenced by an increase in AKT phosphorylation levels and induces the phosphorylation of ERK1/2 in cultured antral follicles. These results demonstrate for the first time that VEGF has a proliferative and cytoprotective role in early antral follicles and in granulosa cells isolated from DES treated prepubertal rats and suggest that PI3K/AKT and ERK/MEK signaling pathways are involved in these processes. © 2010 Elsevier Ireland Ltd. |
| title |
Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells |
| title_short |
Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells |
| title_full |
Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells |
| title_fullStr |
Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells |
| title_full_unstemmed |
Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells |
| title_sort |
direct survival role of vascular endothelial growth factor (vegf) on rat ovarian follicular cells |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v325_n1-2_p93_Irusta http://hdl.handle.net/20.500.12110/paper_03037207_v325_n1-2_p93_Irusta |
| _version_ |
1768546629376278528 |