Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro
The effects of prostaglandins (PGs) on rat pineal metabolism were examined in vitro. PGE2 (0.01-1 μM) increased the activity of serotonin-N-acetyltransferase (SNAT), the stimulation curve exhibiting a maximum at 0.1 μM. PGE1 increased SNAT activity only at the highest dose (1 μm) whereas PGF2α, 15-k...
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1981
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v23_n2_p151_Ritta http://hdl.handle.net/20.500.12110/paper_03037207_v23_n2_p151_Ritta |
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paper:paper_03037207_v23_n2_p151_Ritta2023-06-08T15:29:04Z Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro cAMP cAMP-binding sites melatonin synthesis pineal prostaglandins acyltransferase arylamine acetyltransferase cyclic AMP prostaglandin derivative prostaglandin E prostaglandin E2 aralkylamine acetyltransferase arylamine acetyltransferase cyclic amp drug receptor prostacyclin prostaglandin e2 prostaglandin f2 alpha radioisotope animal article binding site comparative study drug effect enzyme activation in vitro study male metabolism pineal body rat rat strain 15 oxoprostaglandin f2 alpha acetyl coenzyme a c 14 animal experiment cyclic amp h 3 dose response drug response endocrine system pineal body Acetyltransferases Animal Arylamine N-Acetyltransferase Binding Sites Comparative Study Cyclic AMP Dinoprostone Enzyme Activation In Vitro Male Pineal Gland Prostaglandins E Prostaglandins, Synthetic Rats Rats, Inbred Strains Support, Non-U.S. Gov't The effects of prostaglandins (PGs) on rat pineal metabolism were examined in vitro. PGE2 (0.01-1 μM) increased the activity of serotonin-N-acetyltransferase (SNAT), the stimulation curve exhibiting a maximum at 0.1 μM. PGE1 increased SNAT activity only at the highest dose (1 μm) whereas PGF2α, 15-keto-PGF2α or PGI2 did not affect the enzymic activity. The stimulation of SNAT activity brought about by PGE2 in pineals from ganglionectomized rats was greater than in sham-operated controls at all the doses studied, suggesting that the observed effect is predominantly post-synaptic. Only PGE2 significantly increased pineal cAMP accumulation in vitro at doses between 0.01 and 1 μm, and depressed the unoccupied cAMP-binding sites in pineal 900 g supernatants. The total number of cAMP-binding sites remained unaltered after incubation of PGE2. The present observations together with the previously reported NE-induced release of PGs in incubated pineal glands, the occurrence of pineal PG-binding sites and the indomethacin blockade of the nocturnal rise of pineal SNAT and melatonin content, support a role for PGs in the control of melatonin synthesis. © 1981. 1981 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v23_n2_p151_Ritta http://hdl.handle.net/20.500.12110/paper_03037207_v23_n2_p151_Ritta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cAMP cAMP-binding sites melatonin synthesis pineal prostaglandins acyltransferase arylamine acetyltransferase cyclic AMP prostaglandin derivative prostaglandin E prostaglandin E2 aralkylamine acetyltransferase arylamine acetyltransferase cyclic amp drug receptor prostacyclin prostaglandin e2 prostaglandin f2 alpha radioisotope animal article binding site comparative study drug effect enzyme activation in vitro study male metabolism pineal body rat rat strain 15 oxoprostaglandin f2 alpha acetyl coenzyme a c 14 animal experiment cyclic amp h 3 dose response drug response endocrine system pineal body Acetyltransferases Animal Arylamine N-Acetyltransferase Binding Sites Comparative Study Cyclic AMP Dinoprostone Enzyme Activation In Vitro Male Pineal Gland Prostaglandins E Prostaglandins, Synthetic Rats Rats, Inbred Strains Support, Non-U.S. Gov't |
| spellingShingle |
cAMP cAMP-binding sites melatonin synthesis pineal prostaglandins acyltransferase arylamine acetyltransferase cyclic AMP prostaglandin derivative prostaglandin E prostaglandin E2 aralkylamine acetyltransferase arylamine acetyltransferase cyclic amp drug receptor prostacyclin prostaglandin e2 prostaglandin f2 alpha radioisotope animal article binding site comparative study drug effect enzyme activation in vitro study male metabolism pineal body rat rat strain 15 oxoprostaglandin f2 alpha acetyl coenzyme a c 14 animal experiment cyclic amp h 3 dose response drug response endocrine system pineal body Acetyltransferases Animal Arylamine N-Acetyltransferase Binding Sites Comparative Study Cyclic AMP Dinoprostone Enzyme Activation In Vitro Male Pineal Gland Prostaglandins E Prostaglandins, Synthetic Rats Rats, Inbred Strains Support, Non-U.S. Gov't Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro |
| topic_facet |
cAMP cAMP-binding sites melatonin synthesis pineal prostaglandins acyltransferase arylamine acetyltransferase cyclic AMP prostaglandin derivative prostaglandin E prostaglandin E2 aralkylamine acetyltransferase arylamine acetyltransferase cyclic amp drug receptor prostacyclin prostaglandin e2 prostaglandin f2 alpha radioisotope animal article binding site comparative study drug effect enzyme activation in vitro study male metabolism pineal body rat rat strain 15 oxoprostaglandin f2 alpha acetyl coenzyme a c 14 animal experiment cyclic amp h 3 dose response drug response endocrine system pineal body Acetyltransferases Animal Arylamine N-Acetyltransferase Binding Sites Comparative Study Cyclic AMP Dinoprostone Enzyme Activation In Vitro Male Pineal Gland Prostaglandins E Prostaglandins, Synthetic Rats Rats, Inbred Strains Support, Non-U.S. Gov't |
| description |
The effects of prostaglandins (PGs) on rat pineal metabolism were examined in vitro. PGE2 (0.01-1 μM) increased the activity of serotonin-N-acetyltransferase (SNAT), the stimulation curve exhibiting a maximum at 0.1 μM. PGE1 increased SNAT activity only at the highest dose (1 μm) whereas PGF2α, 15-keto-PGF2α or PGI2 did not affect the enzymic activity. The stimulation of SNAT activity brought about by PGE2 in pineals from ganglionectomized rats was greater than in sham-operated controls at all the doses studied, suggesting that the observed effect is predominantly post-synaptic. Only PGE2 significantly increased pineal cAMP accumulation in vitro at doses between 0.01 and 1 μm, and depressed the unoccupied cAMP-binding sites in pineal 900 g supernatants. The total number of cAMP-binding sites remained unaltered after incubation of PGE2. The present observations together with the previously reported NE-induced release of PGs in incubated pineal glands, the occurrence of pineal PG-binding sites and the indomethacin blockade of the nocturnal rise of pineal SNAT and melatonin content, support a role for PGs in the control of melatonin synthesis. © 1981. |
| title |
Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro |
| title_short |
Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro |
| title_full |
Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro |
| title_fullStr |
Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro |
| title_full_unstemmed |
Prostaglandim E2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-N-acetyltransferase activity in rat pineal glands in vitro |
| title_sort |
prostaglandim e2 increases adenosine 3′,5′-monophosphate concentration and binding-site occupancy, and stimulates serotonin-n-acetyltransferase activity in rat pineal glands in vitro |
| publishDate |
1981 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v23_n2_p151_Ritta http://hdl.handle.net/20.500.12110/paper_03037207_v23_n2_p151_Ritta |
| _version_ |
1768543421751885824 |