Nitric oxide donors modify free intracellular calcium levels in rat anterior pituitary cells
The effect of nitric oxide donors on intracellular calcium concentration [Ca2+](i) was studied in anterior pituitary cells using ratiometric FURA 2 fluorescence measurements. Sodium nitroprusside (NP) induced a transient decrease [Ca2+](i), after which [Ca2+](i) returned to, or even increased over b...
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1998
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v146_n1-2_p19_Duvilanski http://hdl.handle.net/20.500.12110/paper_03037207_v146_n1-2_p19_Duvilanski |
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paper:paper_03037207_v146_n1-2_p19_Duvilanski2023-06-08T15:29:00Z Nitric oxide donors modify free intracellular calcium levels in rat anterior pituitary cells Calcium channels Nitric oxide Pituitary calcium ion gallopamil nitric oxide donor nitroprusside sodium s nitrosoglutathione adenohypophysis animal cell article calcium cell level calcium mobilization cell membrane depolarization controlled study hypophysis cell male nonhuman priority journal rat signal transduction Animals Buffers Calcium Calcium Channel Blockers Gallopamil Glutathione Intracellular Fluid Kinetics Male Nitric Oxide Donors Nitroprusside Nitroso Compounds Pituitary Gland, Anterior Potassium Rats Rats, Wistar S-Nitrosoglutathione Thapsigargin The effect of nitric oxide donors on intracellular calcium concentration [Ca2+](i) was studied in anterior pituitary cells using ratiometric FURA 2 fluorescence measurements. Sodium nitroprusside (NP) induced a transient decrease [Ca2+](i), after which [Ca2+](i) returned to, or even increased over basal values. S-Nitroso glutathione (GSNO) induced a similar decrease. NP also inhibited high [Ca2+)(i) achieved by depolarization with 25 mM K+. The inhibitory effect of NP was partially blunted by pretreatment with methoxy-verapamil, and in calcium free buffer, and was not altered by thapsigargin. Interestingly, in calcium free buffer there was a significant stimulatory effect of NP, which was partially blunted by thapsigargin. We conclude that NO donors modify [Ca2+](i) in anterior pituitary cells. The action is biphasic, with an initial decrease in [Ca2+](i) probably related to a decrease of Ca2+ influx through VDCC, and an increase evidenced in calcium free buffer in which the inhibitory component is absent, and partially depends on thapsigargin sensitive calcium stores. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v146_n1-2_p19_Duvilanski http://hdl.handle.net/20.500.12110/paper_03037207_v146_n1-2_p19_Duvilanski |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Calcium channels Nitric oxide Pituitary calcium ion gallopamil nitric oxide donor nitroprusside sodium s nitrosoglutathione adenohypophysis animal cell article calcium cell level calcium mobilization cell membrane depolarization controlled study hypophysis cell male nonhuman priority journal rat signal transduction Animals Buffers Calcium Calcium Channel Blockers Gallopamil Glutathione Intracellular Fluid Kinetics Male Nitric Oxide Donors Nitroprusside Nitroso Compounds Pituitary Gland, Anterior Potassium Rats Rats, Wistar S-Nitrosoglutathione Thapsigargin |
| spellingShingle |
Calcium channels Nitric oxide Pituitary calcium ion gallopamil nitric oxide donor nitroprusside sodium s nitrosoglutathione adenohypophysis animal cell article calcium cell level calcium mobilization cell membrane depolarization controlled study hypophysis cell male nonhuman priority journal rat signal transduction Animals Buffers Calcium Calcium Channel Blockers Gallopamil Glutathione Intracellular Fluid Kinetics Male Nitric Oxide Donors Nitroprusside Nitroso Compounds Pituitary Gland, Anterior Potassium Rats Rats, Wistar S-Nitrosoglutathione Thapsigargin Nitric oxide donors modify free intracellular calcium levels in rat anterior pituitary cells |
| topic_facet |
Calcium channels Nitric oxide Pituitary calcium ion gallopamil nitric oxide donor nitroprusside sodium s nitrosoglutathione adenohypophysis animal cell article calcium cell level calcium mobilization cell membrane depolarization controlled study hypophysis cell male nonhuman priority journal rat signal transduction Animals Buffers Calcium Calcium Channel Blockers Gallopamil Glutathione Intracellular Fluid Kinetics Male Nitric Oxide Donors Nitroprusside Nitroso Compounds Pituitary Gland, Anterior Potassium Rats Rats, Wistar S-Nitrosoglutathione Thapsigargin |
| description |
The effect of nitric oxide donors on intracellular calcium concentration [Ca2+](i) was studied in anterior pituitary cells using ratiometric FURA 2 fluorescence measurements. Sodium nitroprusside (NP) induced a transient decrease [Ca2+](i), after which [Ca2+](i) returned to, or even increased over basal values. S-Nitroso glutathione (GSNO) induced a similar decrease. NP also inhibited high [Ca2+)(i) achieved by depolarization with 25 mM K+. The inhibitory effect of NP was partially blunted by pretreatment with methoxy-verapamil, and in calcium free buffer, and was not altered by thapsigargin. Interestingly, in calcium free buffer there was a significant stimulatory effect of NP, which was partially blunted by thapsigargin. We conclude that NO donors modify [Ca2+](i) in anterior pituitary cells. The action is biphasic, with an initial decrease in [Ca2+](i) probably related to a decrease of Ca2+ influx through VDCC, and an increase evidenced in calcium free buffer in which the inhibitory component is absent, and partially depends on thapsigargin sensitive calcium stores. |
| title |
Nitric oxide donors modify free intracellular calcium levels in rat anterior pituitary cells |
| title_short |
Nitric oxide donors modify free intracellular calcium levels in rat anterior pituitary cells |
| title_full |
Nitric oxide donors modify free intracellular calcium levels in rat anterior pituitary cells |
| title_fullStr |
Nitric oxide donors modify free intracellular calcium levels in rat anterior pituitary cells |
| title_full_unstemmed |
Nitric oxide donors modify free intracellular calcium levels in rat anterior pituitary cells |
| title_sort |
nitric oxide donors modify free intracellular calcium levels in rat anterior pituitary cells |
| publishDate |
1998 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03037207_v146_n1-2_p19_Duvilanski http://hdl.handle.net/20.500.12110/paper_03037207_v146_n1-2_p19_Duvilanski |
| _version_ |
1768542363269988352 |