Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage

The presence of the mutant prophage φ{symbol}105cts23 in Bacillus subtilis strains strongly affected several biological parameters including the viability of protoplasts and the establishment of plasmid pC194. A defective inducibility of the prophage after treatments that de-repress the SOS-like res...

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Autores principales:	Rubinstein, Clara Patricia, Coso, Omar Adrian, Ruzal, Sandra Mónica
Publicado:	1993
Materias:	Bacillus subtilis Bacteriophage φ{symbol}105 Mutant repressor Recombination SOS effects article bacillus subtilis bacteriophage nonhuman osmotic stress priority journal prophage stress virus mutant Bacillus Phages Mutation Plasmids Protoplasts Recombination, Genetic SOS Response (Genetics) Support, Non-U.S. Gov't Transformation, Bacterial
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03028933_v160_n6_p486_Rubinstein http://hdl.handle.net/20.500.12110/paper_03028933_v160_n6_p486_Rubinstein
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03028933_v160_n6_p486_Rubinstein
record_format	dspace
spelling	paper:paper_03028933_v160_n6_p486_Rubinstein2023-06-08T15:28:13Z Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage Rubinstein, Clara Patricia Coso, Omar Adrian Ruzal, Sandra Mónica Bacillus subtilis Bacteriophage φ{symbol}105 Mutant repressor Recombination SOS effects article bacillus subtilis bacteriophage nonhuman osmotic stress priority journal prophage stress virus mutant Bacillus Phages Bacillus subtilis Mutation Plasmids Protoplasts Recombination, Genetic SOS Response (Genetics) Support, Non-U.S. Gov't Transformation, Bacterial Bacillus subtilis The presence of the mutant prophage φ{symbol}105cts23 in Bacillus subtilis strains strongly affected several biological parameters including the viability of protoplasts and the establishment of plasmid pC194. A defective inducibility of the prophage after treatments that de-repress the SOS-like response were also observed. Although these alterations suggested a Rec-deficient phenotype, homologous recombination was not impaired in these lysogenic derivatives. In fact, chromosomal DNA transformation in these competent cells was more efficient than in cells carrying the wild type prophage: cell death due to prophage induction upon competence development was lower than expected. Alterations in the response to SOS-inducing agents and to osmotic stress correlated with the presence of this particular mutant prophage or the cloned thermosensitive repressor at the permissive temperature. The induction of an anti-SOS effect is discussed. © 1993 Springer-Verlag. Fil:Rubinstein, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1993 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03028933_v160_n6_p486_Rubinstein http://hdl.handle.net/20.500.12110/paper_03028933_v160_n6_p486_Rubinstein
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bacillus subtilis Bacteriophage φ{symbol}105 Mutant repressor Recombination SOS effects article bacillus subtilis bacteriophage nonhuman osmotic stress priority journal prophage stress virus mutant Bacillus Phages Bacillus subtilis Mutation Plasmids Protoplasts Recombination, Genetic SOS Response (Genetics) Support, Non-U.S. Gov't Transformation, Bacterial Bacillus subtilis
spellingShingle	Bacillus subtilis Bacteriophage φ{symbol}105 Mutant repressor Recombination SOS effects article bacillus subtilis bacteriophage nonhuman osmotic stress priority journal prophage stress virus mutant Bacillus Phages Bacillus subtilis Mutation Plasmids Protoplasts Recombination, Genetic SOS Response (Genetics) Support, Non-U.S. Gov't Transformation, Bacterial Bacillus subtilis Rubinstein, Clara Patricia Coso, Omar Adrian Ruzal, Sandra Mónica Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage
topic_facet	Bacillus subtilis Bacteriophage φ{symbol}105 Mutant repressor Recombination SOS effects article bacillus subtilis bacteriophage nonhuman osmotic stress priority journal prophage stress virus mutant Bacillus Phages Bacillus subtilis Mutation Plasmids Protoplasts Recombination, Genetic SOS Response (Genetics) Support, Non-U.S. Gov't Transformation, Bacterial Bacillus subtilis
description	The presence of the mutant prophage φ{symbol}105cts23 in Bacillus subtilis strains strongly affected several biological parameters including the viability of protoplasts and the establishment of plasmid pC194. A defective inducibility of the prophage after treatments that de-repress the SOS-like response were also observed. Although these alterations suggested a Rec-deficient phenotype, homologous recombination was not impaired in these lysogenic derivatives. In fact, chromosomal DNA transformation in these competent cells was more efficient than in cells carrying the wild type prophage: cell death due to prophage induction upon competence development was lower than expected. Alterations in the response to SOS-inducing agents and to osmotic stress correlated with the presence of this particular mutant prophage or the cloned thermosensitive repressor at the permissive temperature. The induction of an anti-SOS effect is discussed. © 1993 Springer-Verlag.
author	Rubinstein, Clara Patricia Coso, Omar Adrian Ruzal, Sandra Mónica
author_facet	Rubinstein, Clara Patricia Coso, Omar Adrian Ruzal, Sandra Mónica
author_sort	Rubinstein, Clara Patricia
title	Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage
title_short	Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage
title_full	Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage
title_fullStr	Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage
title_full_unstemmed	Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage
title_sort	anti-sos effects induced in bacillus subtilis by a φ{symbol}105 mutant prophage
publishDate	1993
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03028933_v160_n6_p486_Rubinstein http://hdl.handle.net/20.500.12110/paper_03028933_v160_n6_p486_Rubinstein
work_keys_str_mv	AT rubinsteinclarapatricia antisoseffectsinducedinbacillussubtilisbyaphsymbol105mutantprophage AT cosoomaradrian antisoseffectsinducedinbacillussubtilisbyaphsymbol105mutantprophage AT ruzalsandramonica antisoseffectsinducedinbacillussubtilisbyaphsymbol105mutantprophage
_version_	1768543083214929920

Anti-SOS effects induced in Bacillus subtilis by a φ{symbol}105 mutant prophage

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