Effect of salmon melanin-concentrating hormone and mammalian gonadotrophin-releasing hormone on somatolactin release in pituitary culture of Cichlasoma dimerus

We detected a close morphological association between melanin-concentrating hormone (MCH)-immunoreactive (ir) fibers and somatolactin (SL)-ir cells in the pars intermedia of the cichlid fish Cichlasoma dimerus by double-label immunofluorescence. Male pituitaries obtained from adult C. dimerus were i...

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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0302766X_v333_n1_p49_Canepa
http://hdl.handle.net/20.500.12110/paper_0302766X_v333_n1_p49_Canepa
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spelling paper:paper_0302766X_v333_n1_p49_Canepa2023-06-08T15:28:11Z Effect of salmon melanin-concentrating hormone and mammalian gonadotrophin-releasing hormone on somatolactin release in pituitary culture of Cichlasoma dimerus (Teleostei, Perciformes, Cichlidae) Cichlasoma dimerus Hypothalamus Immunofluorescence Western blot gonadorelin hypophysis hormone melanin concentrating hormone somatolactin animal cell article cell structure Cichlasoma dimerus cichlid controlled study culture medium dose response female hormone release hypophysis intermediate lobe hypothalamus immunofluorescence male mammal nonhuman priority journal salmon Western blotting Cichlasoma Cichlidae Mammalia Perciformes Teleostei We detected a close morphological association between melanin-concentrating hormone (MCH)-immunoreactive (ir) fibers and somatolactin (SL)-ir cells in the pars intermedia of the cichlid fish Cichlasoma dimerus by double-label immunofluorescence. Male pituitaries obtained from adult C. dimerus were incubated with 0.1-10 μM salmon MCH, and the amount of SL released into the culture medium was semi-quantified by Western blot. This assay showed an increase of SL release in a dose-dependent manner (linear regression: P<0.05). A close association of GnRH-ir fibers with SL-ir cells was also detected at the pars intermedia level. Male pituitaries were also incubated with 0.1-10 μM of mammalian GnRH, and SL release was semi-quantified by Western blot, showing an increase of released SL levels in a dose-dependent manner (linear regression: P<0.05). In contrast, SL release was unaffected from female pituitaries incubated with salmon MCH; however, an increasing tendency was observed when mammalian GnRH was used. Hypothalamic close association of MCH-ir perikarya and GnRH-ir fibers was found by double-label immunofluorescence indicating a possible relationship between them. These results suggest that SL, like other pituitary hormones, is under hypothalamic control and is involved in diverse physiological processes including background adaptation and reproduction. This study has also shown that the in vitro culture of a single C. dimerus pituitary is a feasible method for studying the control of SL release and other pituitary hormones. © 2008 Springer-Verlag. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0302766X_v333_n1_p49_Canepa http://hdl.handle.net/20.500.12110/paper_0302766X_v333_n1_p49_Canepa
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic (Teleostei, Perciformes, Cichlidae) Cichlasoma dimerus
Hypothalamus
Immunofluorescence
Western blot
gonadorelin
hypophysis hormone
melanin concentrating hormone
somatolactin
animal cell
article
cell structure
Cichlasoma dimerus
cichlid
controlled study
culture medium
dose response
female
hormone release
hypophysis intermediate lobe
hypothalamus
immunofluorescence
male
mammal
nonhuman
priority journal
salmon
Western blotting
Cichlasoma
Cichlidae
Mammalia
Perciformes
Teleostei
spellingShingle (Teleostei, Perciformes, Cichlidae) Cichlasoma dimerus
Hypothalamus
Immunofluorescence
Western blot
gonadorelin
hypophysis hormone
melanin concentrating hormone
somatolactin
animal cell
article
cell structure
Cichlasoma dimerus
cichlid
controlled study
culture medium
dose response
female
hormone release
hypophysis intermediate lobe
hypothalamus
immunofluorescence
male
mammal
nonhuman
priority journal
salmon
Western blotting
Cichlasoma
Cichlidae
Mammalia
Perciformes
Teleostei
Effect of salmon melanin-concentrating hormone and mammalian gonadotrophin-releasing hormone on somatolactin release in pituitary culture of Cichlasoma dimerus
topic_facet (Teleostei, Perciformes, Cichlidae) Cichlasoma dimerus
Hypothalamus
Immunofluorescence
Western blot
gonadorelin
hypophysis hormone
melanin concentrating hormone
somatolactin
animal cell
article
cell structure
Cichlasoma dimerus
cichlid
controlled study
culture medium
dose response
female
hormone release
hypophysis intermediate lobe
hypothalamus
immunofluorescence
male
mammal
nonhuman
priority journal
salmon
Western blotting
Cichlasoma
Cichlidae
Mammalia
Perciformes
Teleostei
description We detected a close morphological association between melanin-concentrating hormone (MCH)-immunoreactive (ir) fibers and somatolactin (SL)-ir cells in the pars intermedia of the cichlid fish Cichlasoma dimerus by double-label immunofluorescence. Male pituitaries obtained from adult C. dimerus were incubated with 0.1-10 μM salmon MCH, and the amount of SL released into the culture medium was semi-quantified by Western blot. This assay showed an increase of SL release in a dose-dependent manner (linear regression: P<0.05). A close association of GnRH-ir fibers with SL-ir cells was also detected at the pars intermedia level. Male pituitaries were also incubated with 0.1-10 μM of mammalian GnRH, and SL release was semi-quantified by Western blot, showing an increase of released SL levels in a dose-dependent manner (linear regression: P<0.05). In contrast, SL release was unaffected from female pituitaries incubated with salmon MCH; however, an increasing tendency was observed when mammalian GnRH was used. Hypothalamic close association of MCH-ir perikarya and GnRH-ir fibers was found by double-label immunofluorescence indicating a possible relationship between them. These results suggest that SL, like other pituitary hormones, is under hypothalamic control and is involved in diverse physiological processes including background adaptation and reproduction. This study has also shown that the in vitro culture of a single C. dimerus pituitary is a feasible method for studying the control of SL release and other pituitary hormones. © 2008 Springer-Verlag.
title Effect of salmon melanin-concentrating hormone and mammalian gonadotrophin-releasing hormone on somatolactin release in pituitary culture of Cichlasoma dimerus
title_short Effect of salmon melanin-concentrating hormone and mammalian gonadotrophin-releasing hormone on somatolactin release in pituitary culture of Cichlasoma dimerus
title_full Effect of salmon melanin-concentrating hormone and mammalian gonadotrophin-releasing hormone on somatolactin release in pituitary culture of Cichlasoma dimerus
title_fullStr Effect of salmon melanin-concentrating hormone and mammalian gonadotrophin-releasing hormone on somatolactin release in pituitary culture of Cichlasoma dimerus
title_full_unstemmed Effect of salmon melanin-concentrating hormone and mammalian gonadotrophin-releasing hormone on somatolactin release in pituitary culture of Cichlasoma dimerus
title_sort effect of salmon melanin-concentrating hormone and mammalian gonadotrophin-releasing hormone on somatolactin release in pituitary culture of cichlasoma dimerus
publishDate 2008
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0302766X_v333_n1_p49_Canepa
http://hdl.handle.net/20.500.12110/paper_0302766X_v333_n1_p49_Canepa
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