GnRH systems of Cichlasoma dimerus (Perciformes, Cichlidae) revisited: A localization study with antibodies and riboprobes to GnRH-associated peptides

The distribution of cells that express three prepro-gonadotropin-releasing hormones (GnRH), corresponding to salmon GnRH, sea bream GnRH (sbGnRH), and chicken II GnRH, was studied in the brain and pituitary of the South American cichlid fish, Cichlasoma dimerus. Although the ontogeny and distributio...

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Autores principales:	Pandolfi, Matías, Lo Nostro, Fabiana Laura, Paz, Dante Agustin, Maggese, María Cristina Irene
Publicado:	2005
Materias:	Brain Cichlasoma dimerus (Telestei) Cichlids GAP GnRH gonadorelin gonadorelin antibody gonadorelin associated peptide derivative animal cell animal tissue antibody detection article cichlasoma dimerus cichlid cross reaction female forebrain hypophysis immunohistochemistry male mesencephalon molecular probe nerve cell nonhuman olfactory bulb olfactory nucleus ontogeny Perciformes preoptic area priority journal rhombencephalon RNA probe salmon sea bream telencephalon Animals Female Gonadotropin-Releasing Hormone Immunohistochemistry Male Neural Pathways Neurons Olfactory Bulb Oligonucleotide Probes Peptides Pituitary Gland Preoptic Area Telencephalon Archosargus rhomboidalis Cichlasoma Cichlidae
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0302766X_v321_n2_p219_Pandolfi http://hdl.handle.net/20.500.12110/paper_0302766X_v321_n2_p219_Pandolfi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0302766X_v321_n2_p219_Pandolfi
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spelling	paper:paper_0302766X_v321_n2_p219_Pandolfi2023-06-08T15:28:11Z GnRH systems of Cichlasoma dimerus (Perciformes, Cichlidae) revisited: A localization study with antibodies and riboprobes to GnRH-associated peptides Pandolfi, Matías Lo Nostro, Fabiana Laura Paz, Dante Agustin Maggese, María Cristina Irene Brain Cichlasoma dimerus (Telestei) Cichlids GAP GnRH gonadorelin gonadorelin antibody gonadorelin associated peptide derivative animal cell animal tissue antibody detection article cichlasoma dimerus cichlid cross reaction female forebrain hypophysis immunohistochemistry male mesencephalon molecular probe nerve cell nonhuman olfactory bulb olfactory nucleus ontogeny Perciformes preoptic area priority journal rhombencephalon RNA probe salmon sea bream telencephalon Animals Brain Cichlids Female Gonadotropin-Releasing Hormone Immunohistochemistry Male Neural Pathways Neurons Olfactory Bulb Oligonucleotide Probes Peptides Pituitary Gland Preoptic Area Telencephalon Archosargus rhomboidalis Cichlasoma Cichlidae Perciformes The distribution of cells that express three prepro-gonadotropin-releasing hormones (GnRH), corresponding to salmon GnRH, sea bream GnRH (sbGnRH), and chicken II GnRH, was studied in the brain and pituitary of the South American cichlid fish, Cichlasoma dimerus. Although the ontogeny and distribution of GnRH neuronal systems have previously been examined immunohistochemically with antibodies and antisera against the various GnRH decapeptides, we have used antisera against various perciform GnRH-associated peptides (GAPs) and riboprobes to various perciform GnRH+GAPs. The results demonstrate that: (1) the GnRH neuronal populations in the forebrain (salmon and sea bream GAPs; sGAP and sbGAP, respectively) show an overlapping pattern along the olfactory bulbs, nucleus olfacto-retinalis, ventral telencephalon, and preoptic area; (2) projections with sGAP are mainly located in the forebrain and contribute to the pituitary innervation, with projections containing chicken GAP II being mainly distributed along the mid and hindbrain and not contributing to pituitary innervation, whereas sbGAP projections are restricted to the ventral forebrain, being the most important molecular form in relation to pituitary innervation; (3) sbGnRH (GnRH I) neurons have an olfactory origin; (4) GAP antibodies and GAP riboprobes are valuable tools for the study of various GnRH systems, by avoiding the cross-reactivity problems that occur when using GnRH antibodies and GnRH riboprobes alone. © Springer-Verlag 2005. Fil:Pandolfi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maggese, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0302766X_v321_n2_p219_Pandolfi http://hdl.handle.net/20.500.12110/paper_0302766X_v321_n2_p219_Pandolfi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Brain Cichlasoma dimerus (Telestei) Cichlids GAP GnRH gonadorelin gonadorelin antibody gonadorelin associated peptide derivative animal cell animal tissue antibody detection article cichlasoma dimerus cichlid cross reaction female forebrain hypophysis immunohistochemistry male mesencephalon molecular probe nerve cell nonhuman olfactory bulb olfactory nucleus ontogeny Perciformes preoptic area priority journal rhombencephalon RNA probe salmon sea bream telencephalon Animals Brain Cichlids Female Gonadotropin-Releasing Hormone Immunohistochemistry Male Neural Pathways Neurons Olfactory Bulb Oligonucleotide Probes Peptides Pituitary Gland Preoptic Area Telencephalon Archosargus rhomboidalis Cichlasoma Cichlidae Perciformes
spellingShingle	Brain Cichlasoma dimerus (Telestei) Cichlids GAP GnRH gonadorelin gonadorelin antibody gonadorelin associated peptide derivative animal cell animal tissue antibody detection article cichlasoma dimerus cichlid cross reaction female forebrain hypophysis immunohistochemistry male mesencephalon molecular probe nerve cell nonhuman olfactory bulb olfactory nucleus ontogeny Perciformes preoptic area priority journal rhombencephalon RNA probe salmon sea bream telencephalon Animals Brain Cichlids Female Gonadotropin-Releasing Hormone Immunohistochemistry Male Neural Pathways Neurons Olfactory Bulb Oligonucleotide Probes Peptides Pituitary Gland Preoptic Area Telencephalon Archosargus rhomboidalis Cichlasoma Cichlidae Perciformes Pandolfi, Matías Lo Nostro, Fabiana Laura Paz, Dante Agustin Maggese, María Cristina Irene GnRH systems of Cichlasoma dimerus (Perciformes, Cichlidae) revisited: A localization study with antibodies and riboprobes to GnRH-associated peptides
topic_facet	Brain Cichlasoma dimerus (Telestei) Cichlids GAP GnRH gonadorelin gonadorelin antibody gonadorelin associated peptide derivative animal cell animal tissue antibody detection article cichlasoma dimerus cichlid cross reaction female forebrain hypophysis immunohistochemistry male mesencephalon molecular probe nerve cell nonhuman olfactory bulb olfactory nucleus ontogeny Perciformes preoptic area priority journal rhombencephalon RNA probe salmon sea bream telencephalon Animals Brain Cichlids Female Gonadotropin-Releasing Hormone Immunohistochemistry Male Neural Pathways Neurons Olfactory Bulb Oligonucleotide Probes Peptides Pituitary Gland Preoptic Area Telencephalon Archosargus rhomboidalis Cichlasoma Cichlidae Perciformes
description	The distribution of cells that express three prepro-gonadotropin-releasing hormones (GnRH), corresponding to salmon GnRH, sea bream GnRH (sbGnRH), and chicken II GnRH, was studied in the brain and pituitary of the South American cichlid fish, Cichlasoma dimerus. Although the ontogeny and distribution of GnRH neuronal systems have previously been examined immunohistochemically with antibodies and antisera against the various GnRH decapeptides, we have used antisera against various perciform GnRH-associated peptides (GAPs) and riboprobes to various perciform GnRH+GAPs. The results demonstrate that: (1) the GnRH neuronal populations in the forebrain (salmon and sea bream GAPs; sGAP and sbGAP, respectively) show an overlapping pattern along the olfactory bulbs, nucleus olfacto-retinalis, ventral telencephalon, and preoptic area; (2) projections with sGAP are mainly located in the forebrain and contribute to the pituitary innervation, with projections containing chicken GAP II being mainly distributed along the mid and hindbrain and not contributing to pituitary innervation, whereas sbGAP projections are restricted to the ventral forebrain, being the most important molecular form in relation to pituitary innervation; (3) sbGnRH (GnRH I) neurons have an olfactory origin; (4) GAP antibodies and GAP riboprobes are valuable tools for the study of various GnRH systems, by avoiding the cross-reactivity problems that occur when using GnRH antibodies and GnRH riboprobes alone. © Springer-Verlag 2005.
author	Pandolfi, Matías Lo Nostro, Fabiana Laura Paz, Dante Agustin Maggese, María Cristina Irene
author_facet	Pandolfi, Matías Lo Nostro, Fabiana Laura Paz, Dante Agustin Maggese, María Cristina Irene
author_sort	Pandolfi, Matías
title	GnRH systems of Cichlasoma dimerus (Perciformes, Cichlidae) revisited: A localization study with antibodies and riboprobes to GnRH-associated peptides
title_short	GnRH systems of Cichlasoma dimerus (Perciformes, Cichlidae) revisited: A localization study with antibodies and riboprobes to GnRH-associated peptides
title_full	GnRH systems of Cichlasoma dimerus (Perciformes, Cichlidae) revisited: A localization study with antibodies and riboprobes to GnRH-associated peptides
title_fullStr	GnRH systems of Cichlasoma dimerus (Perciformes, Cichlidae) revisited: A localization study with antibodies and riboprobes to GnRH-associated peptides
title_full_unstemmed	GnRH systems of Cichlasoma dimerus (Perciformes, Cichlidae) revisited: A localization study with antibodies and riboprobes to GnRH-associated peptides
title_sort	gnrh systems of cichlasoma dimerus (perciformes, cichlidae) revisited: a localization study with antibodies and riboprobes to gnrh-associated peptides
publishDate	2005
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0302766X_v321_n2_p219_Pandolfi http://hdl.handle.net/20.500.12110/paper_0302766X_v321_n2_p219_Pandolfi
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_version_	1768544594036785152

GnRH systems of Cichlasoma dimerus (Perciformes, Cichlidae) revisited: A localization study with antibodies and riboprobes to GnRH-associated peptides

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