Polyamines and antibiotic effects on translation

Protein synthesis was studied in polyamine-auxotrophic mutants of E. coli. The decreased protein synthesizing rate observed both in vivo and in vitro in polyamine-starved bacteria is due to defective 30S ribosomal subparticles which are impaired in the initiation step of translation. Analysis of pep...

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Publicado:	1981
Materias:	kanamycin kasugamycin neomycin polyamine putrescine radioisotope spermidine spermine streptomycin tetracycline cell malformation dihydrostreptomycin h 3 dose response drug comparison drug response escherichia coli in vitro study leucine c 14 methionine s 35 phenylalanine c 14 protein synthesis Aminoglycosides Antibiotics Bacterial Proteins Cell-Free System Escherichia coli Polyamines Putrescine Ribosomes RNA, Transfer, Amino Acyl Streptomycin Support, Non-U.S. Gov't Translation, Genetic
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03022137_v59_n5-6_p360_Goldemberg http://hdl.handle.net/20.500.12110/paper_03022137_v59_n5-6_p360_Goldemberg
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03022137_v59_n5-6_p360_Goldemberg
record_format	dspace
spelling	paper:paper_03022137_v59_n5-6_p360_Goldemberg2023-06-08T15:28:09Z Polyamines and antibiotic effects on translation kanamycin kasugamycin neomycin polyamine putrescine radioisotope spermidine spermine streptomycin tetracycline cell malformation dihydrostreptomycin h 3 dose response drug comparison drug response escherichia coli in vitro study leucine c 14 methionine s 35 phenylalanine c 14 protein synthesis Aminoglycosides Antibiotics Bacterial Proteins Cell-Free System Escherichia coli Polyamines Putrescine Ribosomes RNA, Transfer, Amino Acyl Streptomycin Support, Non-U.S. Gov't Translation, Genetic Protein synthesis was studied in polyamine-auxotrophic mutants of E. coli. The decreased protein synthesizing rate observed both in vivo and in vitro in polyamine-starved bacteria is due to defective 30S ribosomal subparticles which are impaired in the initiation step of translation. Analysis of peptides synthesized in vivo suggests a more extensive misreading by putrescine-depleted bacteria. Cells grown without polyamine show a markedly decreased response to the inhibitory effect and the misreading inducing action of streptomycin in vivo and in vitro. The polyamine-starved bacteria are also less sensitive to other aminoglycoside antibiotics such as neomycin, kanamycin and kasugamycin. 1981 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03022137_v59_n5-6_p360_Goldemberg http://hdl.handle.net/20.500.12110/paper_03022137_v59_n5-6_p360_Goldemberg
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	kanamycin kasugamycin neomycin polyamine putrescine radioisotope spermidine spermine streptomycin tetracycline cell malformation dihydrostreptomycin h 3 dose response drug comparison drug response escherichia coli in vitro study leucine c 14 methionine s 35 phenylalanine c 14 protein synthesis Aminoglycosides Antibiotics Bacterial Proteins Cell-Free System Escherichia coli Polyamines Putrescine Ribosomes RNA, Transfer, Amino Acyl Streptomycin Support, Non-U.S. Gov't Translation, Genetic
spellingShingle	kanamycin kasugamycin neomycin polyamine putrescine radioisotope spermidine spermine streptomycin tetracycline cell malformation dihydrostreptomycin h 3 dose response drug comparison drug response escherichia coli in vitro study leucine c 14 methionine s 35 phenylalanine c 14 protein synthesis Aminoglycosides Antibiotics Bacterial Proteins Cell-Free System Escherichia coli Polyamines Putrescine Ribosomes RNA, Transfer, Amino Acyl Streptomycin Support, Non-U.S. Gov't Translation, Genetic Polyamines and antibiotic effects on translation
topic_facet	kanamycin kasugamycin neomycin polyamine putrescine radioisotope spermidine spermine streptomycin tetracycline cell malformation dihydrostreptomycin h 3 dose response drug comparison drug response escherichia coli in vitro study leucine c 14 methionine s 35 phenylalanine c 14 protein synthesis Aminoglycosides Antibiotics Bacterial Proteins Cell-Free System Escherichia coli Polyamines Putrescine Ribosomes RNA, Transfer, Amino Acyl Streptomycin Support, Non-U.S. Gov't Translation, Genetic
description	Protein synthesis was studied in polyamine-auxotrophic mutants of E. coli. The decreased protein synthesizing rate observed both in vivo and in vitro in polyamine-starved bacteria is due to defective 30S ribosomal subparticles which are impaired in the initiation step of translation. Analysis of peptides synthesized in vivo suggests a more extensive misreading by putrescine-depleted bacteria. Cells grown without polyamine show a markedly decreased response to the inhibitory effect and the misreading inducing action of streptomycin in vivo and in vitro. The polyamine-starved bacteria are also less sensitive to other aminoglycoside antibiotics such as neomycin, kanamycin and kasugamycin.
title	Polyamines and antibiotic effects on translation
title_short	Polyamines and antibiotic effects on translation
title_full	Polyamines and antibiotic effects on translation
title_fullStr	Polyamines and antibiotic effects on translation
title_full_unstemmed	Polyamines and antibiotic effects on translation
title_sort	polyamines and antibiotic effects on translation
publishDate	1981
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03022137_v59_n5-6_p360_Goldemberg http://hdl.handle.net/20.500.12110/paper_03022137_v59_n5-6_p360_Goldemberg
_version_	1768544776456503296

Polyamines and antibiotic effects on translation

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