Effects of estradiol on norepinephrine and prostaglandin efflux in medial basal hypothalamus of ovariectomized rats

The spontaneous and K+-stimulated efflux of norepinephrine (NE) and the release of PGE2 and PGF2α were examined in medial basal hypothalamus (MBH) of ovariectomized rats killed before and during the LH release that follows estradiol treatment. As compared to vehicle-treated, ovariectomized rats, est...

Descripción completa

Guardado en:
Detalles Bibliográficos
Publicado: 1982
Materias:
estradiol
LH release
Medial basal hypothalamus
norepinephrine
prostaglandins
luteinizing hormone
noradrenalin
prostaglandin
radioisotope
animal experiment
basal thalamus
central nervous system
endocrine system
hypothalamus
noradrenalin h 3
ovariectomy
rat
Animal
Castration
Dinoprost
Estradiol
Female
Hypothalamus
Luteinizing Hormone
Norepinephrine
Prostaglandins E
Prostaglandins F
Radioimmunoassay
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03009564_v53_n1_p39_Cardinali
http://hdl.handle.net/20.500.12110/paper_03009564_v53_n1_p39_Cardinali
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03009564_v53_n1_p39_Cardinali
record_format dspace
spelling paper:paper_03009564_v53_n1_p39_Cardinali2023-06-08T15:27:40Z Effects of estradiol on norepinephrine and prostaglandin efflux in medial basal hypothalamus of ovariectomized rats estradiol LH release Medial basal hypothalamus norepinephrine prostaglandins estradiol luteinizing hormone noradrenalin prostaglandin radioisotope animal experiment basal thalamus central nervous system endocrine system hypothalamus noradrenalin h 3 ovariectomy rat Animal Castration Dinoprost Estradiol Female Hypothalamus Luteinizing Hormone Norepinephrine Prostaglandins E Prostaglandins F Radioimmunoassay Rats Rats, Inbred Strains Support, Non-U.S. Gov't The spontaneous and K+-stimulated efflux of norepinephrine (NE) and the release of PGE2 and PGF2α were examined in medial basal hypothalamus (MBH) of ovariectomized rats killed before and during the LH release that follows estradiol treatment. As compared to vehicle-treated, ovariectomized rats, estradiol-primed rats exhibited a 60% more increase in K+-stimulated3H-overflow of MBH slices preloaded with3H-NE at morning hours (1000 hours). Estradiol treatment did not result in further increase of K+-induced3H release from MBH slices at the time of LH release (1700 hours), nor affected labeled NE release in occipital cortex slices. A significant difference between K+-stimulated NE release of vehicle-treated spayed rats killed at 1000 and 1700 hours was observed, the latter showing 54% more release upon stimulus. PGE2 efflux was time-dependent being highest at the evening in both vehicle- and estradiol-treated animals. The MBH of estrogenized rats released significantly more PGE2 at the evening as compared to the controls. The release of PGF2α remained essentially unchanged regardless of estradiol treatment or time of day. The present results offer additional support to the involvement of MBH catecholamines and prostaglandins in the mechanism of LH secretion in the rat. © 1982 Springer-Verlag. 1982 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03009564_v53_n1_p39_Cardinali http://hdl.handle.net/20.500.12110/paper_03009564_v53_n1_p39_Cardinali
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic estradiol
LH release
Medial basal hypothalamus
norepinephrine
prostaglandins
estradiol
luteinizing hormone
noradrenalin
prostaglandin
radioisotope
animal experiment
basal thalamus
central nervous system
endocrine system
hypothalamus
noradrenalin h 3
ovariectomy
rat
Animal
Castration
Dinoprost
Estradiol
Female
Hypothalamus
Luteinizing Hormone
Norepinephrine
Prostaglandins E
Prostaglandins F
Radioimmunoassay
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
spellingShingle estradiol
LH release
Medial basal hypothalamus
norepinephrine
prostaglandins
estradiol
luteinizing hormone
noradrenalin
prostaglandin
radioisotope
animal experiment
basal thalamus
central nervous system
endocrine system
hypothalamus
noradrenalin h 3
ovariectomy
rat
Animal
Castration
Dinoprost
Estradiol
Female
Hypothalamus
Luteinizing Hormone
Norepinephrine
Prostaglandins E
Prostaglandins F
Radioimmunoassay
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
Effects of estradiol on norepinephrine and prostaglandin efflux in medial basal hypothalamus of ovariectomized rats
topic_facet estradiol
LH release
Medial basal hypothalamus
norepinephrine
prostaglandins
estradiol
luteinizing hormone
noradrenalin
prostaglandin
radioisotope
animal experiment
basal thalamus
central nervous system
endocrine system
hypothalamus
noradrenalin h 3
ovariectomy
rat
Animal
Castration
Dinoprost
Estradiol
Female
Hypothalamus
Luteinizing Hormone
Norepinephrine
Prostaglandins E
Prostaglandins F
Radioimmunoassay
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
description The spontaneous and K+-stimulated efflux of norepinephrine (NE) and the release of PGE2 and PGF2α were examined in medial basal hypothalamus (MBH) of ovariectomized rats killed before and during the LH release that follows estradiol treatment. As compared to vehicle-treated, ovariectomized rats, estradiol-primed rats exhibited a 60% more increase in K+-stimulated3H-overflow of MBH slices preloaded with3H-NE at morning hours (1000 hours). Estradiol treatment did not result in further increase of K+-induced3H release from MBH slices at the time of LH release (1700 hours), nor affected labeled NE release in occipital cortex slices. A significant difference between K+-stimulated NE release of vehicle-treated spayed rats killed at 1000 and 1700 hours was observed, the latter showing 54% more release upon stimulus. PGE2 efflux was time-dependent being highest at the evening in both vehicle- and estradiol-treated animals. The MBH of estrogenized rats released significantly more PGE2 at the evening as compared to the controls. The release of PGF2α remained essentially unchanged regardless of estradiol treatment or time of day. The present results offer additional support to the involvement of MBH catecholamines and prostaglandins in the mechanism of LH secretion in the rat. © 1982 Springer-Verlag.
title Effects of estradiol on norepinephrine and prostaglandin efflux in medial basal hypothalamus of ovariectomized rats
title_short Effects of estradiol on norepinephrine and prostaglandin efflux in medial basal hypothalamus of ovariectomized rats
title_full Effects of estradiol on norepinephrine and prostaglandin efflux in medial basal hypothalamus of ovariectomized rats
title_fullStr Effects of estradiol on norepinephrine and prostaglandin efflux in medial basal hypothalamus of ovariectomized rats
title_full_unstemmed Effects of estradiol on norepinephrine and prostaglandin efflux in medial basal hypothalamus of ovariectomized rats
title_sort effects of estradiol on norepinephrine and prostaglandin efflux in medial basal hypothalamus of ovariectomized rats
publishDate 1982
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03009564_v53_n1_p39_Cardinali
http://hdl.handle.net/20.500.12110/paper_03009564_v53_n1_p39_Cardinali
_version_ 1768544223788793856

Ejemplares similares