Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes

Insulin-like growth factor-1 (IGF-I) is an essential factor for the differentiation of preadipocytes into adipocytes. We investigated the expression of IGF-I receptor and IGF-I RNA messenger during 3T3-L1 preadipocyte differentiation. Levels of IGF-I receptor decreased in the mature adipocytes compa...

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Detalles Bibliográficos
Publicado: 2002
Materias:
Adipocytes
Differentiation
IGF-I receptor
Insulin-like growth factor-1
insulin
messenger RNA
somatomedin C
somatomedin C receptor
adipocyte
animal cell
article
autocrine effect
cell culture
cell differentiation
cell maturation
controlled study
embryo
in vitro study
model
mouse
nonhuman
paracrine signaling
proadipocyte
protein expression
protein synthesis
3T3 Cells
Animals
Cell Differentiation
Gene Expression Regulation
Insulin
Insulin-Like Growth Factor I
Mice
Receptor, IGF Type 1
RNA, Messenger
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03009084_v84_n10_p975_Zizola
http://hdl.handle.net/20.500.12110/paper_03009084_v84_n10_p975_Zizola
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Insulin-like growth factor-1 (IGF-I) is an essential factor for the differentiation of preadipocytes into adipocytes. We investigated the expression of IGF-I receptor and IGF-I RNA messenger during 3T3-L1 preadipocyte differentiation. Levels of IGF-I receptor decreased in the mature adipocytes compared to cells before the initiation of differentiation. In addition, cultures not induced to differentiate showed a decrease on the receptor levels after 4 days in the presence of insulin compared to cultures without treatment. The levels of the IGF-I RNA messenger were shown to be higher in mature adipocytes compared to preadipocytes. We propose an autocrine and/or paracrine action of IGF-I in this adipocyte differentiation model, where IGF-I produced by the differentiating preadipocytes acts over their adjacent cells and, in this way, diminishes the expression of IGF-I receptor. © 2002 Éditions scientifiques et médicales Elsevier SAS and Société française de biochimie et biologie moléculaire. All rights reserved.

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