Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes
Insulin-like growth factor-1 (IGF-I) is an essential factor for the differentiation of preadipocytes into adipocytes. We investigated the expression of IGF-I receptor and IGF-I RNA messenger during 3T3-L1 preadipocyte differentiation. Levels of IGF-I receptor decreased in the mature adipocytes compa...
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2002
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03009084_v84_n10_p975_Zizola http://hdl.handle.net/20.500.12110/paper_03009084_v84_n10_p975_Zizola |
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paper:paper_03009084_v84_n10_p975_Zizola2023-06-08T15:27:34Z Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes Adipocytes Differentiation IGF-I receptor Insulin-like growth factor-1 insulin messenger RNA somatomedin C somatomedin C receptor adipocyte animal cell article autocrine effect cell culture cell differentiation cell maturation controlled study embryo in vitro study model mouse nonhuman paracrine signaling proadipocyte protein expression protein synthesis 3T3 Cells Adipocytes Animals Cell Differentiation Gene Expression Regulation Insulin Insulin-Like Growth Factor I Mice Receptor, IGF Type 1 RNA, Messenger Insulin-like growth factor-1 (IGF-I) is an essential factor for the differentiation of preadipocytes into adipocytes. We investigated the expression of IGF-I receptor and IGF-I RNA messenger during 3T3-L1 preadipocyte differentiation. Levels of IGF-I receptor decreased in the mature adipocytes compared to cells before the initiation of differentiation. In addition, cultures not induced to differentiate showed a decrease on the receptor levels after 4 days in the presence of insulin compared to cultures without treatment. The levels of the IGF-I RNA messenger were shown to be higher in mature adipocytes compared to preadipocytes. We propose an autocrine and/or paracrine action of IGF-I in this adipocyte differentiation model, where IGF-I produced by the differentiating preadipocytes acts over their adjacent cells and, in this way, diminishes the expression of IGF-I receptor. © 2002 Éditions scientifiques et médicales Elsevier SAS and Société française de biochimie et biologie moléculaire. All rights reserved. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03009084_v84_n10_p975_Zizola http://hdl.handle.net/20.500.12110/paper_03009084_v84_n10_p975_Zizola |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Adipocytes Differentiation IGF-I receptor Insulin-like growth factor-1 insulin messenger RNA somatomedin C somatomedin C receptor adipocyte animal cell article autocrine effect cell culture cell differentiation cell maturation controlled study embryo in vitro study model mouse nonhuman paracrine signaling proadipocyte protein expression protein synthesis 3T3 Cells Adipocytes Animals Cell Differentiation Gene Expression Regulation Insulin Insulin-Like Growth Factor I Mice Receptor, IGF Type 1 RNA, Messenger |
spellingShingle |
Adipocytes Differentiation IGF-I receptor Insulin-like growth factor-1 insulin messenger RNA somatomedin C somatomedin C receptor adipocyte animal cell article autocrine effect cell culture cell differentiation cell maturation controlled study embryo in vitro study model mouse nonhuman paracrine signaling proadipocyte protein expression protein synthesis 3T3 Cells Adipocytes Animals Cell Differentiation Gene Expression Regulation Insulin Insulin-Like Growth Factor I Mice Receptor, IGF Type 1 RNA, Messenger Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes |
topic_facet |
Adipocytes Differentiation IGF-I receptor Insulin-like growth factor-1 insulin messenger RNA somatomedin C somatomedin C receptor adipocyte animal cell article autocrine effect cell culture cell differentiation cell maturation controlled study embryo in vitro study model mouse nonhuman paracrine signaling proadipocyte protein expression protein synthesis 3T3 Cells Adipocytes Animals Cell Differentiation Gene Expression Regulation Insulin Insulin-Like Growth Factor I Mice Receptor, IGF Type 1 RNA, Messenger |
description |
Insulin-like growth factor-1 (IGF-I) is an essential factor for the differentiation of preadipocytes into adipocytes. We investigated the expression of IGF-I receptor and IGF-I RNA messenger during 3T3-L1 preadipocyte differentiation. Levels of IGF-I receptor decreased in the mature adipocytes compared to cells before the initiation of differentiation. In addition, cultures not induced to differentiate showed a decrease on the receptor levels after 4 days in the presence of insulin compared to cultures without treatment. The levels of the IGF-I RNA messenger were shown to be higher in mature adipocytes compared to preadipocytes. We propose an autocrine and/or paracrine action of IGF-I in this adipocyte differentiation model, where IGF-I produced by the differentiating preadipocytes acts over their adjacent cells and, in this way, diminishes the expression of IGF-I receptor. © 2002 Éditions scientifiques et médicales Elsevier SAS and Société française de biochimie et biologie moléculaire. All rights reserved. |
title |
Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes |
title_short |
Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes |
title_full |
Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes |
title_fullStr |
Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes |
title_full_unstemmed |
Changes in IGF-I receptor and IGF-I mRNA during differentiation of 3T3-L1 preadipocytes |
title_sort |
changes in igf-i receptor and igf-i mrna during differentiation of 3t3-l1 preadipocytes |
publishDate |
2002 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03009084_v84_n10_p975_Zizola http://hdl.handle.net/20.500.12110/paper_03009084_v84_n10_p975_Zizola |
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1768545092858019840 |