A novel stimulator of protein phosphorylation in Neurospora crassa

An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or...

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Autores principales: Glikin, Gerardo Claudio, Torres, Héctor Norberto, Judewicz, Norberto D.
Publicado: 1987
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v77_n1_p11_Ulloa
http://hdl.handle.net/20.500.12110/paper_03008177_v77_n1_p11_Ulloa
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spelling paper:paper_03008177_v77_n1_p11_Ulloa2023-06-08T15:27:31Z A novel stimulator of protein phosphorylation in Neurospora crassa Glikin, Gerardo Claudio Torres, Héctor Norberto Judewicz, Norberto D. Neurospora crassa protein kinase regulation fungal protein protein kinase article enzyme activation enzymology gel chromatography growth, development and aging kinetics metabolism molecular weight Neurospora Neurospora crassa phosphorylation Chromatography, Gel Enzyme Activation Fungal Proteins Kinetics Molecular Weight Neurospora Neurospora crassa Phosphorylation Protein Kinases An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin "in vitro" phosphorylation. The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed. © 1987 Martinus Nijhoff Publishers. Fil:Glikin, G.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Judewicz, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1987 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v77_n1_p11_Ulloa http://hdl.handle.net/20.500.12110/paper_03008177_v77_n1_p11_Ulloa
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Neurospora crassa
protein kinase regulation
fungal protein
protein kinase
article
enzyme activation
enzymology
gel chromatography
growth, development and aging
kinetics
metabolism
molecular weight
Neurospora
Neurospora crassa
phosphorylation
Chromatography, Gel
Enzyme Activation
Fungal Proteins
Kinetics
Molecular Weight
Neurospora
Neurospora crassa
Phosphorylation
Protein Kinases
spellingShingle Neurospora crassa
protein kinase regulation
fungal protein
protein kinase
article
enzyme activation
enzymology
gel chromatography
growth, development and aging
kinetics
metabolism
molecular weight
Neurospora
Neurospora crassa
phosphorylation
Chromatography, Gel
Enzyme Activation
Fungal Proteins
Kinetics
Molecular Weight
Neurospora
Neurospora crassa
Phosphorylation
Protein Kinases
Glikin, Gerardo Claudio
Torres, Héctor Norberto
Judewicz, Norberto D.
A novel stimulator of protein phosphorylation in Neurospora crassa
topic_facet Neurospora crassa
protein kinase regulation
fungal protein
protein kinase
article
enzyme activation
enzymology
gel chromatography
growth, development and aging
kinetics
metabolism
molecular weight
Neurospora
Neurospora crassa
phosphorylation
Chromatography, Gel
Enzyme Activation
Fungal Proteins
Kinetics
Molecular Weight
Neurospora
Neurospora crassa
Phosphorylation
Protein Kinases
description An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin "in vitro" phosphorylation. The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed. © 1987 Martinus Nijhoff Publishers.
author Glikin, Gerardo Claudio
Torres, Héctor Norberto
Judewicz, Norberto D.
author_facet Glikin, Gerardo Claudio
Torres, Héctor Norberto
Judewicz, Norberto D.
author_sort Glikin, Gerardo Claudio
title A novel stimulator of protein phosphorylation in Neurospora crassa
title_short A novel stimulator of protein phosphorylation in Neurospora crassa
title_full A novel stimulator of protein phosphorylation in Neurospora crassa
title_fullStr A novel stimulator of protein phosphorylation in Neurospora crassa
title_full_unstemmed A novel stimulator of protein phosphorylation in Neurospora crassa
title_sort novel stimulator of protein phosphorylation in neurospora crassa
publishDate 1987
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v77_n1_p11_Ulloa
http://hdl.handle.net/20.500.12110/paper_03008177_v77_n1_p11_Ulloa
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