A novel stimulator of protein phosphorylation in Neurospora crassa
An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or...
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1987
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v77_n1_p11_Ulloa http://hdl.handle.net/20.500.12110/paper_03008177_v77_n1_p11_Ulloa |
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paper:paper_03008177_v77_n1_p11_Ulloa2023-06-08T15:27:31Z A novel stimulator of protein phosphorylation in Neurospora crassa Glikin, Gerardo Claudio Torres, Héctor Norberto Judewicz, Norberto D. Neurospora crassa protein kinase regulation fungal protein protein kinase article enzyme activation enzymology gel chromatography growth, development and aging kinetics metabolism molecular weight Neurospora Neurospora crassa phosphorylation Chromatography, Gel Enzyme Activation Fungal Proteins Kinetics Molecular Weight Neurospora Neurospora crassa Phosphorylation Protein Kinases An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin "in vitro" phosphorylation. The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed. © 1987 Martinus Nijhoff Publishers. Fil:Glikin, G.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Judewicz, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1987 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v77_n1_p11_Ulloa http://hdl.handle.net/20.500.12110/paper_03008177_v77_n1_p11_Ulloa |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Neurospora crassa protein kinase regulation fungal protein protein kinase article enzyme activation enzymology gel chromatography growth, development and aging kinetics metabolism molecular weight Neurospora Neurospora crassa phosphorylation Chromatography, Gel Enzyme Activation Fungal Proteins Kinetics Molecular Weight Neurospora Neurospora crassa Phosphorylation Protein Kinases |
spellingShingle |
Neurospora crassa protein kinase regulation fungal protein protein kinase article enzyme activation enzymology gel chromatography growth, development and aging kinetics metabolism molecular weight Neurospora Neurospora crassa phosphorylation Chromatography, Gel Enzyme Activation Fungal Proteins Kinetics Molecular Weight Neurospora Neurospora crassa Phosphorylation Protein Kinases Glikin, Gerardo Claudio Torres, Héctor Norberto Judewicz, Norberto D. A novel stimulator of protein phosphorylation in Neurospora crassa |
topic_facet |
Neurospora crassa protein kinase regulation fungal protein protein kinase article enzyme activation enzymology gel chromatography growth, development and aging kinetics metabolism molecular weight Neurospora Neurospora crassa phosphorylation Chromatography, Gel Enzyme Activation Fungal Proteins Kinetics Molecular Weight Neurospora Neurospora crassa Phosphorylation Protein Kinases |
description |
An endogenous thermostable activator of Protein kinase III (PKIII) was purified from 100000 × g supernatants of Neurospora crassa mycelial extracts. This 38 000 dalton polypeptide, clearly separable from calmodulin on P-60 gel filtration, specifically stimulated N. crassa PKIII activity on casein or phosvitin "in vitro" phosphorylation. The factor was only present in the initial growth phase of the fungus. The mechanism of PKIII activation and its possible regulatory role are discussed. © 1987 Martinus Nijhoff Publishers. |
author |
Glikin, Gerardo Claudio Torres, Héctor Norberto Judewicz, Norberto D. |
author_facet |
Glikin, Gerardo Claudio Torres, Héctor Norberto Judewicz, Norberto D. |
author_sort |
Glikin, Gerardo Claudio |
title |
A novel stimulator of protein phosphorylation in Neurospora crassa |
title_short |
A novel stimulator of protein phosphorylation in Neurospora crassa |
title_full |
A novel stimulator of protein phosphorylation in Neurospora crassa |
title_fullStr |
A novel stimulator of protein phosphorylation in Neurospora crassa |
title_full_unstemmed |
A novel stimulator of protein phosphorylation in Neurospora crassa |
title_sort |
novel stimulator of protein phosphorylation in neurospora crassa |
publishDate |
1987 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v77_n1_p11_Ulloa http://hdl.handle.net/20.500.12110/paper_03008177_v77_n1_p11_Ulloa |
work_keys_str_mv |
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_version_ |
1768545646398144512 |