Cellular and molecular oxidative stress-related effects in uterine myometrial and trophoblast-decidual tissues after perigestational alcohol intake up to early mouse organogenesis
The placenta plays a major role in embryo-fetal defects and intrauterine growth retardation after maternal alcohol consumption. Our aims were to determine the oxidative status and cellular and molecular oxidative stress effects on uterine myometrium and trophoblast-decidual tissue following perigest...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v440_n1-2_p89_Coll http://hdl.handle.net/20.500.12110/paper_03008177_v440_n1-2_p89_Coll |
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paper:paper_03008177_v440_n1-2_p89_Coll2023-06-08T15:27:28Z Cellular and molecular oxidative stress-related effects in uterine myometrial and trophoblast-decidual tissues after perigestational alcohol intake up to early mouse organogenesis Cellular and tissue damage Decidua Mouse organogenesis Oxidative stress Perigestational alcohol Placenta 3 nitrotyrosine alcohol catalase drinking water glutathione glutathione transferase nitrite superoxide dismutase thiobarbituric acid reactive substance adult alcohol consumption animal experiment animal model animal tissue apoptosis Article cell nucleus controlled study decidua endothelium enzyme activity female giant cell histopathology immunohistochemistry immunoreactivity macromolecule morphometry mouse myometrium nidation nonhuman organogenesis oxidative stress pregnancy trophoblast animal decidua fetal alcohol syndrome maternal exposure metabolism myometrium pathology pregnancy trophoblast Animals Decidua Female Fetal Alcohol Spectrum Disorders Maternal Exposure Mice Myometrium Organogenesis Oxidative Stress Pregnancy Trophoblasts The placenta plays a major role in embryo-fetal defects and intrauterine growth retardation after maternal alcohol consumption. Our aims were to determine the oxidative status and cellular and molecular oxidative stress effects on uterine myometrium and trophoblast-decidual tissue following perigestational alcohol intake at early organogenesis. CF-1 female mice were administered with 10% alcohol in drinking water for 17 days prior to and up to day 10 of gestation. Control females received ethanol-free water. Treated mice had smaller implantation sites compared to controls (p < 0.05), diminished maternal vascular lumen, and irregular/discontinuous endothelium of decidual vessels. The trophoblast giant cell layer was disorganized and presented increased abnormal nuclear frequency. The myometrium of treated females had reduced nitrite content, increased superoxide dismutase activity, and reduced glutathione (GSH) content (p < 0.05). However, the trophoblast-decidual tissue of treated females had increased nitrite content (p < 0.05), increased GSH level (p < 0.001), increased thiobarbituric acid-reactive substance concentration (p < 0.001), higher 3-nitrotyrosine immunoreaction, and increased apoptotic index (p < 0.05) compared to controls. In summary, perigestational alcohol ingestion at organogenesis induced oxidative stress in the myometrium and trophoblast-decidual tissue, mainly affecting cells and macromolecules of trophoblast and decidual tissues around early organogenesis, in CF-1 mouse, and suggests that oxidative-induced abnormal early placental formation probably leads to risk of prematurity and fetal growth impairment at term. © 2017, Springer Science+Business Media, LLC. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v440_n1-2_p89_Coll http://hdl.handle.net/20.500.12110/paper_03008177_v440_n1-2_p89_Coll |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cellular and tissue damage Decidua Mouse organogenesis Oxidative stress Perigestational alcohol Placenta 3 nitrotyrosine alcohol catalase drinking water glutathione glutathione transferase nitrite superoxide dismutase thiobarbituric acid reactive substance adult alcohol consumption animal experiment animal model animal tissue apoptosis Article cell nucleus controlled study decidua endothelium enzyme activity female giant cell histopathology immunohistochemistry immunoreactivity macromolecule morphometry mouse myometrium nidation nonhuman organogenesis oxidative stress pregnancy trophoblast animal decidua fetal alcohol syndrome maternal exposure metabolism myometrium pathology pregnancy trophoblast Animals Decidua Female Fetal Alcohol Spectrum Disorders Maternal Exposure Mice Myometrium Organogenesis Oxidative Stress Pregnancy Trophoblasts |
| spellingShingle |
Cellular and tissue damage Decidua Mouse organogenesis Oxidative stress Perigestational alcohol Placenta 3 nitrotyrosine alcohol catalase drinking water glutathione glutathione transferase nitrite superoxide dismutase thiobarbituric acid reactive substance adult alcohol consumption animal experiment animal model animal tissue apoptosis Article cell nucleus controlled study decidua endothelium enzyme activity female giant cell histopathology immunohistochemistry immunoreactivity macromolecule morphometry mouse myometrium nidation nonhuman organogenesis oxidative stress pregnancy trophoblast animal decidua fetal alcohol syndrome maternal exposure metabolism myometrium pathology pregnancy trophoblast Animals Decidua Female Fetal Alcohol Spectrum Disorders Maternal Exposure Mice Myometrium Organogenesis Oxidative Stress Pregnancy Trophoblasts Cellular and molecular oxidative stress-related effects in uterine myometrial and trophoblast-decidual tissues after perigestational alcohol intake up to early mouse organogenesis |
| topic_facet |
Cellular and tissue damage Decidua Mouse organogenesis Oxidative stress Perigestational alcohol Placenta 3 nitrotyrosine alcohol catalase drinking water glutathione glutathione transferase nitrite superoxide dismutase thiobarbituric acid reactive substance adult alcohol consumption animal experiment animal model animal tissue apoptosis Article cell nucleus controlled study decidua endothelium enzyme activity female giant cell histopathology immunohistochemistry immunoreactivity macromolecule morphometry mouse myometrium nidation nonhuman organogenesis oxidative stress pregnancy trophoblast animal decidua fetal alcohol syndrome maternal exposure metabolism myometrium pathology pregnancy trophoblast Animals Decidua Female Fetal Alcohol Spectrum Disorders Maternal Exposure Mice Myometrium Organogenesis Oxidative Stress Pregnancy Trophoblasts |
| description |
The placenta plays a major role in embryo-fetal defects and intrauterine growth retardation after maternal alcohol consumption. Our aims were to determine the oxidative status and cellular and molecular oxidative stress effects on uterine myometrium and trophoblast-decidual tissue following perigestational alcohol intake at early organogenesis. CF-1 female mice were administered with 10% alcohol in drinking water for 17 days prior to and up to day 10 of gestation. Control females received ethanol-free water. Treated mice had smaller implantation sites compared to controls (p < 0.05), diminished maternal vascular lumen, and irregular/discontinuous endothelium of decidual vessels. The trophoblast giant cell layer was disorganized and presented increased abnormal nuclear frequency. The myometrium of treated females had reduced nitrite content, increased superoxide dismutase activity, and reduced glutathione (GSH) content (p < 0.05). However, the trophoblast-decidual tissue of treated females had increased nitrite content (p < 0.05), increased GSH level (p < 0.001), increased thiobarbituric acid-reactive substance concentration (p < 0.001), higher 3-nitrotyrosine immunoreaction, and increased apoptotic index (p < 0.05) compared to controls. In summary, perigestational alcohol ingestion at organogenesis induced oxidative stress in the myometrium and trophoblast-decidual tissue, mainly affecting cells and macromolecules of trophoblast and decidual tissues around early organogenesis, in CF-1 mouse, and suggests that oxidative-induced abnormal early placental formation probably leads to risk of prematurity and fetal growth impairment at term. © 2017, Springer Science+Business Media, LLC. |
| title |
Cellular and molecular oxidative stress-related effects in uterine myometrial and trophoblast-decidual tissues after perigestational alcohol intake up to early mouse organogenesis |
| title_short |
Cellular and molecular oxidative stress-related effects in uterine myometrial and trophoblast-decidual tissues after perigestational alcohol intake up to early mouse organogenesis |
| title_full |
Cellular and molecular oxidative stress-related effects in uterine myometrial and trophoblast-decidual tissues after perigestational alcohol intake up to early mouse organogenesis |
| title_fullStr |
Cellular and molecular oxidative stress-related effects in uterine myometrial and trophoblast-decidual tissues after perigestational alcohol intake up to early mouse organogenesis |
| title_full_unstemmed |
Cellular and molecular oxidative stress-related effects in uterine myometrial and trophoblast-decidual tissues after perigestational alcohol intake up to early mouse organogenesis |
| title_sort |
cellular and molecular oxidative stress-related effects in uterine myometrial and trophoblast-decidual tissues after perigestational alcohol intake up to early mouse organogenesis |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v440_n1-2_p89_Coll http://hdl.handle.net/20.500.12110/paper_03008177_v440_n1-2_p89_Coll |
| _version_ |
1768544911585443840 |