Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations

Cytosolic adenylate cyclase activity from rat seminiferous tubules was purified by chromatography in DEAE-cellulose, hydroxylapatite and Bio-Gel A-0.5 m as well as by centrifugation in sucrose gradients. In all these purification steps, fractions with adenylate cyclase activity also contained bindin...

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Autores principales: Kornblihtt, Alberto Rodolfo, Flawiá, Mirtha María, Glikin, Gerardo Claudio, Farías, Ricardo Norberto, Torres, Héctor Norberto
Publicado: 1981
Materias:
adenylate cyclase
liothyronine
animal experiment
male genital system
rat
testis
Adenylate Cyclase
Animals
Cytosol
Kinetics
Male
Protein Binding
Rats
Seminiferous Tubules
Testis
Triiodothyronine
Animalia
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v36_n1_p23_Kornblihtt
http://hdl.handle.net/20.500.12110/paper_03008177_v36_n1_p23_Kornblihtt
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03008177_v36_n1_p23_Kornblihtt
record_format dspace
spelling paper:paper_03008177_v36_n1_p23_Kornblihtt2023-06-08T15:27:26Z Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations Kornblihtt, Alberto Rodolfo Flawiá, Mirtha María Glikin, Gerardo Claudio Farías, Ricardo Norberto Torres, Héctor Norberto adenylate cyclase liothyronine animal experiment male genital system rat testis Adenylate Cyclase Animals Cytosol Kinetics Male Protein Binding Rats Seminiferous Tubules Testis Triiodothyronine Animalia Cytosolic adenylate cyclase activity from rat seminiferous tubules was purified by chromatography in DEAE-cellulose, hydroxylapatite and Bio-Gel A-0.5 m as well as by centrifugation in sucrose gradients. In all these purification steps, fractions with adenylate cyclase activity also contained binding activity for L-T3. Binding studies indicate the existence of two L-T3 receptor components associated to adenylate cyclase activity. The component exhibiting the highest hormone affinity has the lowest binding capacity. © 1981 Martinus Nijhoff/Dr W. Junk Publishers. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Glikin, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Farías, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1981 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v36_n1_p23_Kornblihtt http://hdl.handle.net/20.500.12110/paper_03008177_v36_n1_p23_Kornblihtt
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic adenylate cyclase
liothyronine
animal experiment
male genital system
rat
testis
Adenylate Cyclase
Animals
Cytosol
Kinetics
Male
Protein Binding
Rats
Seminiferous Tubules
Testis
Triiodothyronine
Animalia
spellingShingle adenylate cyclase
liothyronine
animal experiment
male genital system
rat
testis
Adenylate Cyclase
Animals
Cytosol
Kinetics
Male
Protein Binding
Rats
Seminiferous Tubules
Testis
Triiodothyronine
Animalia
Kornblihtt, Alberto Rodolfo
Flawiá, Mirtha María
Glikin, Gerardo Claudio
Farías, Ricardo Norberto
Torres, Héctor Norberto
Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations
topic_facet adenylate cyclase
liothyronine
animal experiment
male genital system
rat
testis
Adenylate Cyclase
Animals
Cytosol
Kinetics
Male
Protein Binding
Rats
Seminiferous Tubules
Testis
Triiodothyronine
Animalia
description Cytosolic adenylate cyclase activity from rat seminiferous tubules was purified by chromatography in DEAE-cellulose, hydroxylapatite and Bio-Gel A-0.5 m as well as by centrifugation in sucrose gradients. In all these purification steps, fractions with adenylate cyclase activity also contained binding activity for L-T3. Binding studies indicate the existence of two L-T3 receptor components associated to adenylate cyclase activity. The component exhibiting the highest hormone affinity has the lowest binding capacity. © 1981 Martinus Nijhoff/Dr W. Junk Publishers.
author Kornblihtt, Alberto Rodolfo
Flawiá, Mirtha María
Glikin, Gerardo Claudio
Farías, Ricardo Norberto
Torres, Héctor Norberto
author_facet Kornblihtt, Alberto Rodolfo
Flawiá, Mirtha María
Glikin, Gerardo Claudio
Farías, Ricardo Norberto
Torres, Héctor Norberto
author_sort Kornblihtt, Alberto Rodolfo
title Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations
title_short Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations
title_full Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations
title_fullStr Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations
title_full_unstemmed Association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations
title_sort association of triiodothyronine binding activity to soluble adenylate cyclase in testicular preparations
publishDate 1981
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v36_n1_p23_Kornblihtt
http://hdl.handle.net/20.500.12110/paper_03008177_v36_n1_p23_Kornblihtt
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AT flawiamirthamaria associationoftriiodothyroninebindingactivitytosolubleadenylatecyclaseintesticularpreparations
AT glikingerardoclaudio associationoftriiodothyroninebindingactivitytosolubleadenylatecyclaseintesticularpreparations
AT fariasricardonorberto associationoftriiodothyroninebindingactivitytosolubleadenylatecyclaseintesticularpreparations
AT torreshectornorberto associationoftriiodothyroninebindingactivitytosolubleadenylatecyclaseintesticularpreparations
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