Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine
Cytosolic adenylate cyclase activity from rat seminiferous tubules is inhibited by L-triiodothyronine (L-T3). In a typical dose-response curve, using Mn-ATP as substrate, no effect is observed at 10-10 M L-T3; about 15 to 25% inhibition is found in the range between 10-9 and 10-6 M L-T3 and finally...
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1981
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v34_n3_p185_Flawia http://hdl.handle.net/20.500.12110/paper_03008177_v34_n3_p185_Flawia |
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paper:paper_03008177_v34_n3_p185_Flawia2023-06-08T15:27:26Z Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine Flawiá, Mirtha María Kornblihtt, Alberto Rodolfo Torres, Héctor Norberto adenylate cyclase cyclic amp liothyronine animal experiment drug response endocrine system enzyme inhibition hormone sensitivity in vitro study male genital system preliminary communication rat seminiferous tubule testis Adenylate Cyclase Animal Cyclic AMP Cytosol Dose-Response Relationship, Drug Hydrogen-Ion Concentration Kinetics Magnesium Male Manganese Rats Subcellular Fractions Support, Non-U.S. Gov't Testis Triiodothyronine Cytosolic adenylate cyclase activity from rat seminiferous tubules is inhibited by L-triiodothyronine (L-T3). In a typical dose-response curve, using Mn-ATP as substrate, no effect is observed at 10-10 M L-T3; about 15 to 25% inhibition is found in the range between 10-9 and 10-6 M L-T3 and finally a sharp enzyme inhibition is evident at increasing hormone concentrations from 10-6 to 10-4 M. Incubation of decapsulated testes with L-T3 leads to a decrease of intracellular cyclic AMP levels. Dose-response relationships for such effect are similar to those found for adenylate cyclase activity. In this case a clear response is observed at 10-8 M L-T3. © 1981 Martinus Nijhoff Dr. W. Juk Publishers B.V. Fil:Flawia, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1981 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v34_n3_p185_Flawia http://hdl.handle.net/20.500.12110/paper_03008177_v34_n3_p185_Flawia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adenylate cyclase cyclic amp liothyronine animal experiment drug response endocrine system enzyme inhibition hormone sensitivity in vitro study male genital system preliminary communication rat seminiferous tubule testis Adenylate Cyclase Animal Cyclic AMP Cytosol Dose-Response Relationship, Drug Hydrogen-Ion Concentration Kinetics Magnesium Male Manganese Rats Subcellular Fractions Support, Non-U.S. Gov't Testis Triiodothyronine |
| spellingShingle |
adenylate cyclase cyclic amp liothyronine animal experiment drug response endocrine system enzyme inhibition hormone sensitivity in vitro study male genital system preliminary communication rat seminiferous tubule testis Adenylate Cyclase Animal Cyclic AMP Cytosol Dose-Response Relationship, Drug Hydrogen-Ion Concentration Kinetics Magnesium Male Manganese Rats Subcellular Fractions Support, Non-U.S. Gov't Testis Triiodothyronine Flawiá, Mirtha María Kornblihtt, Alberto Rodolfo Torres, Héctor Norberto Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine |
| topic_facet |
adenylate cyclase cyclic amp liothyronine animal experiment drug response endocrine system enzyme inhibition hormone sensitivity in vitro study male genital system preliminary communication rat seminiferous tubule testis Adenylate Cyclase Animal Cyclic AMP Cytosol Dose-Response Relationship, Drug Hydrogen-Ion Concentration Kinetics Magnesium Male Manganese Rats Subcellular Fractions Support, Non-U.S. Gov't Testis Triiodothyronine |
| description |
Cytosolic adenylate cyclase activity from rat seminiferous tubules is inhibited by L-triiodothyronine (L-T3). In a typical dose-response curve, using Mn-ATP as substrate, no effect is observed at 10-10 M L-T3; about 15 to 25% inhibition is found in the range between 10-9 and 10-6 M L-T3 and finally a sharp enzyme inhibition is evident at increasing hormone concentrations from 10-6 to 10-4 M. Incubation of decapsulated testes with L-T3 leads to a decrease of intracellular cyclic AMP levels. Dose-response relationships for such effect are similar to those found for adenylate cyclase activity. In this case a clear response is observed at 10-8 M L-T3. © 1981 Martinus Nijhoff Dr. W. Juk Publishers B.V. |
| author |
Flawiá, Mirtha María Kornblihtt, Alberto Rodolfo Torres, Héctor Norberto |
| author_facet |
Flawiá, Mirtha María Kornblihtt, Alberto Rodolfo Torres, Héctor Norberto |
| author_sort |
Flawiá, Mirtha María |
| title |
Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine |
| title_short |
Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine |
| title_full |
Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine |
| title_fullStr |
Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine |
| title_full_unstemmed |
Inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by L-triiodothyronine |
| title_sort |
inhibition of cyclic 3′5′ monophosphate synthesis in rat testis by l-triiodothyronine |
| publishDate |
1981 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v34_n3_p185_Flawia http://hdl.handle.net/20.500.12110/paper_03008177_v34_n3_p185_Flawia |
| work_keys_str_mv |
AT flawiamirthamaria inhibitionofcyclic35monophosphatesynthesisinrattestisbyltriiodothyronine AT kornblihttalbertorodolfo inhibitionofcyclic35monophosphatesynthesisinrattestisbyltriiodothyronine AT torreshectornorberto inhibitionofcyclic35monophosphatesynthesisinrattestisbyltriiodothyronine |
| _version_ |
1768543750964903936 |