Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum

A particulate enzyme preparation from Acetobacter xylinum synthesizes ficaprenol-monophosphate-β-galactose from ficaprenol monophosphate (FMP) and UDP-galactose in the presence of detergent. The product has the same properties as those previously reported for the compound formed with the endogenous...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Romero Martinez, Pedro Antonio, García, Rodolfo Carlos, Dankert, Marcelo Alberto
Publicado: 1977
Materias:
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p205_Romero
http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p205_Romero
Aporte de:
id paper:paper_03008177_v16_n2-3_p205_Romero
record_format dspace
spelling paper:paper_03008177_v16_n2-3_p205_Romero2023-06-08T15:27:23Z Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum Romero Martinez, Pedro Antonio García, Rodolfo Carlos Dankert, Marcelo Alberto acetobacter xylinum beta galactoside derivative in vitro study microorganism polyprenol derivative theoretical study Acetobacter Hexosephosphates Kinetics Polyethylene Glycols Polysaccharides Support, U.S. Gov't, P.H.S. Terpenes Uridine Diphosphate Galactose A particulate enzyme preparation from Acetobacter xylinum synthesizes ficaprenol-monophosphate-β-galactose from ficaprenol monophosphate (FMP) and UDP-galactose in the presence of detergent. The product has the same properties as those previously reported for the compound formed with the endogenous acceptor. Dolichol-monophosphate (DolMP) is also a good galactose acceptor but the product obtained has different properties. Lipid extracts from Acetobacter contain galactose acceptor capacity which is lost by mild acid treatment. FMP behaves in a similar manner but DolMP is resistant to this treatment. It is concluded that the endogeneous acceptor is an allylic phosphate ester of a polyprenol. © 1977 Dr. W. Junk b.v. Publishers. Fil:Romero, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Garcia, R.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dankert, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1977 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p205_Romero http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p205_Romero
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic acetobacter xylinum
beta galactoside derivative
in vitro study
microorganism
polyprenol derivative
theoretical study
Acetobacter
Hexosephosphates
Kinetics
Polyethylene Glycols
Polysaccharides
Support, U.S. Gov't, P.H.S.
Terpenes
Uridine Diphosphate Galactose
spellingShingle acetobacter xylinum
beta galactoside derivative
in vitro study
microorganism
polyprenol derivative
theoretical study
Acetobacter
Hexosephosphates
Kinetics
Polyethylene Glycols
Polysaccharides
Support, U.S. Gov't, P.H.S.
Terpenes
Uridine Diphosphate Galactose
Romero Martinez, Pedro Antonio
García, Rodolfo Carlos
Dankert, Marcelo Alberto
Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum
topic_facet acetobacter xylinum
beta galactoside derivative
in vitro study
microorganism
polyprenol derivative
theoretical study
Acetobacter
Hexosephosphates
Kinetics
Polyethylene Glycols
Polysaccharides
Support, U.S. Gov't, P.H.S.
Terpenes
Uridine Diphosphate Galactose
description A particulate enzyme preparation from Acetobacter xylinum synthesizes ficaprenol-monophosphate-β-galactose from ficaprenol monophosphate (FMP) and UDP-galactose in the presence of detergent. The product has the same properties as those previously reported for the compound formed with the endogenous acceptor. Dolichol-monophosphate (DolMP) is also a good galactose acceptor but the product obtained has different properties. Lipid extracts from Acetobacter contain galactose acceptor capacity which is lost by mild acid treatment. FMP behaves in a similar manner but DolMP is resistant to this treatment. It is concluded that the endogeneous acceptor is an allylic phosphate ester of a polyprenol. © 1977 Dr. W. Junk b.v. Publishers.
author Romero Martinez, Pedro Antonio
García, Rodolfo Carlos
Dankert, Marcelo Alberto
author_facet Romero Martinez, Pedro Antonio
García, Rodolfo Carlos
Dankert, Marcelo Alberto
author_sort Romero Martinez, Pedro Antonio
title Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum
title_short Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum
title_full Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum
title_fullStr Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum
title_full_unstemmed Synthesis of polyprenol-monophosphate-β-galactose by Acetobacter xylinum
title_sort synthesis of polyprenol-monophosphate-β-galactose by acetobacter xylinum
publishDate 1977
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p205_Romero
http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p205_Romero
work_keys_str_mv AT romeromartinezpedroantonio synthesisofpolyprenolmonophosphatebgalactosebyacetobacterxylinum
AT garciarodolfocarlos synthesisofpolyprenolmonophosphatebgalactosebyacetobacterxylinum
AT dankertmarceloalberto synthesisofpolyprenolmonophosphatebgalactosebyacetobacterxylinum
_version_ 1768546540436062208