Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells
Incubation of cells from a wild type strain of E. coli with 0.3 mg/ml rifampicin for 15 minutes lead to a complete inhibition of RNA synthesis measured as the uracil incorporation into the trichloroacetic acid insoluble fraction. In these rifampicin-treated cells [14C]uracil incorporation tended to...
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1977
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p135_Judewicz http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p135_Judewicz |
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paper:paper_03008177_v16_n2-3_p135_Judewicz2023-06-08T15:27:22Z Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells Judewicz, Norberto D. Torres, Héctor Norberto cyclic amp radioisotope rifampicin rna uracil cell membrane transport dose time effect relation escherichia coli in vitro study microorganism rna synthesis uracil c 14 Adenosine Monophosphate Adenosine Triphosphate Biological Transport, Active Cyclic AMP Escherichia coli Kinetics Rifampin RNA, Bacterial Transcription, Genetic Uracil Incubation of cells from a wild type strain of E. coli with 0.3 mg/ml rifampicin for 15 minutes lead to a complete inhibition of RNA synthesis measured as the uracil incorporation into the trichloroacetic acid insoluble fraction. In these rifampicin-treated cells [14C]uracil incorporation tended to decrease during a further incubation at 37°. Addition of cyclic AMP increased the inactivation of the system responsible for [14C]uracil uptake. The cyclic nucleotide effect seems to be specific since ATP or 5′AMP did not increase such inactivation. © 1977 Dr. W. Junk b.v. Publishers. Fil:Judewicz, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1977 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p135_Judewicz http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p135_Judewicz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cyclic amp radioisotope rifampicin rna uracil cell membrane transport dose time effect relation escherichia coli in vitro study microorganism rna synthesis uracil c 14 Adenosine Monophosphate Adenosine Triphosphate Biological Transport, Active Cyclic AMP Escherichia coli Kinetics Rifampin RNA, Bacterial Transcription, Genetic Uracil |
| spellingShingle |
cyclic amp radioisotope rifampicin rna uracil cell membrane transport dose time effect relation escherichia coli in vitro study microorganism rna synthesis uracil c 14 Adenosine Monophosphate Adenosine Triphosphate Biological Transport, Active Cyclic AMP Escherichia coli Kinetics Rifampin RNA, Bacterial Transcription, Genetic Uracil Judewicz, Norberto D. Torres, Héctor Norberto Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells |
| topic_facet |
cyclic amp radioisotope rifampicin rna uracil cell membrane transport dose time effect relation escherichia coli in vitro study microorganism rna synthesis uracil c 14 Adenosine Monophosphate Adenosine Triphosphate Biological Transport, Active Cyclic AMP Escherichia coli Kinetics Rifampin RNA, Bacterial Transcription, Genetic Uracil |
| description |
Incubation of cells from a wild type strain of E. coli with 0.3 mg/ml rifampicin for 15 minutes lead to a complete inhibition of RNA synthesis measured as the uracil incorporation into the trichloroacetic acid insoluble fraction. In these rifampicin-treated cells [14C]uracil incorporation tended to decrease during a further incubation at 37°. Addition of cyclic AMP increased the inactivation of the system responsible for [14C]uracil uptake. The cyclic nucleotide effect seems to be specific since ATP or 5′AMP did not increase such inactivation. © 1977 Dr. W. Junk b.v. Publishers. |
| author |
Judewicz, Norberto D. Torres, Héctor Norberto |
| author_facet |
Judewicz, Norberto D. Torres, Héctor Norberto |
| author_sort |
Judewicz, Norberto D. |
| title |
Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells |
| title_short |
Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells |
| title_full |
Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells |
| title_fullStr |
Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells |
| title_full_unstemmed |
Influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated Escherichia coli cells |
| title_sort |
influence of cyclic 3′,5′-adenosine monophosphate on uracil uptake by rifampicin treated escherichia coli cells |
| publishDate |
1977 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v16_n2-3_p135_Judewicz http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p135_Judewicz |
| work_keys_str_mv |
AT judewicznorbertod influenceofcyclic35adenosinemonophosphateonuraciluptakebyrifampicintreatedescherichiacolicells AT torreshectornorberto influenceofcyclic35adenosinemonophosphateonuraciluptakebyrifampicintreatedescherichiacolicells |
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1768542691356835840 |