Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa
DEAE-cellulose column chromatography of Neurospora crassa soluble mycelial extracts leads to the resolution of three major protein kinase activity peaks designated PKI, PKII, and PKIII. PKII activity is stimulated by Ca2+ and Neurospora or brain calmodulin. Maximal stimulation was observed at 2 μM-f...
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1991
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v102_n2_p155_Ulloa http://hdl.handle.net/20.500.12110/paper_03008177_v102_n2_p155_Ulloa |
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paper:paper_03008177_v102_n2_p155_Ulloa2023-06-08T15:27:21Z Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa Torres, Héctor Norberto Ochatt, Claudia Margarita autophosphorylation Ca2+ calmodulin kinase Neurospora crassa protein kinase (calcium,calmodulin) Ca(2+) Calmodulin Dependent Protein Kinase calcium calmodulin cyclic AMP egtazic acid fungal protein protein kinase protein kinase (calcium,calmodulin) article autophosphorylation neurospora crassa nonhuman priority journal enzymology gel chromatography ion exchange chromatography isolation and purification Neurospora crassa phosphorylation polyacrylamide gel electrophoresis protein processing Ascomycota Neurospora Neurospora crassa Ca(2+)-Calmodulin Dependent Protein Kinase Calcium Calmodulin Chromatography, DEAE-Cellulose Chromatography, Gel Cyclic AMP Egtazic Acid Electrophoresis, Polyacrylamide Gel Fungal Proteins Neurospora crassa Phosphorylation Protein Kinases Protein Processing, Post-Translational Support, Non-U.S. Gov't DEAE-cellulose column chromatography of Neurospora crassa soluble mycelial extracts leads to the resolution of three major protein kinase activity peaks designated PKI, PKII, and PKIII. PKII activity is stimulated by Ca2+ and Neurospora or brain calmodulin. Maximal stimulation was observed at 2 μM-free Ca2+ and 1 μg/ml of the modulator. The stimulatory effect of the Ca2+-calmodulin complex was blocked by EGTA and by some calmodulin antagonists such as phenothiazine drugs or compound 48/80. PKII phosphorylates different proteins, among which histone II-A at a low concentration and CDPKS, the synthetic peptide specific for Ca2+-calmodulin dependent protein kinases, are the best substrates. Some phosphorylation can be detected in the absence of any exogenous acceptor. PKII activity assayed in the presence of histone II-A or in the absence of exogenous phosphate acceptor (autophosphorylation) co-elute in a DEAE-cellulose column at 0.28 M NaCl. As result of the autophosphorylation reaction of the purified enzyme a main phosphorylated component of 70 kDa was resolved by SDS-polyacrylamide gel electrophoresis. It is possible that this component is an active part of this enzyme. © 1991 Kluwer Academic Publishers. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ochatt, C.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1991 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v102_n2_p155_Ulloa http://hdl.handle.net/20.500.12110/paper_03008177_v102_n2_p155_Ulloa |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
autophosphorylation Ca2+ calmodulin kinase Neurospora crassa protein kinase (calcium,calmodulin) Ca(2+) Calmodulin Dependent Protein Kinase calcium calmodulin cyclic AMP egtazic acid fungal protein protein kinase protein kinase (calcium,calmodulin) article autophosphorylation neurospora crassa nonhuman priority journal enzymology gel chromatography ion exchange chromatography isolation and purification Neurospora crassa phosphorylation polyacrylamide gel electrophoresis protein processing Ascomycota Neurospora Neurospora crassa Ca(2+)-Calmodulin Dependent Protein Kinase Calcium Calmodulin Chromatography, DEAE-Cellulose Chromatography, Gel Cyclic AMP Egtazic Acid Electrophoresis, Polyacrylamide Gel Fungal Proteins Neurospora crassa Phosphorylation Protein Kinases Protein Processing, Post-Translational Support, Non-U.S. Gov't |
| spellingShingle |
autophosphorylation Ca2+ calmodulin kinase Neurospora crassa protein kinase (calcium,calmodulin) Ca(2+) Calmodulin Dependent Protein Kinase calcium calmodulin cyclic AMP egtazic acid fungal protein protein kinase protein kinase (calcium,calmodulin) article autophosphorylation neurospora crassa nonhuman priority journal enzymology gel chromatography ion exchange chromatography isolation and purification Neurospora crassa phosphorylation polyacrylamide gel electrophoresis protein processing Ascomycota Neurospora Neurospora crassa Ca(2+)-Calmodulin Dependent Protein Kinase Calcium Calmodulin Chromatography, DEAE-Cellulose Chromatography, Gel Cyclic AMP Egtazic Acid Electrophoresis, Polyacrylamide Gel Fungal Proteins Neurospora crassa Phosphorylation Protein Kinases Protein Processing, Post-Translational Support, Non-U.S. Gov't Torres, Héctor Norberto Ochatt, Claudia Margarita Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa |
| topic_facet |
autophosphorylation Ca2+ calmodulin kinase Neurospora crassa protein kinase (calcium,calmodulin) Ca(2+) Calmodulin Dependent Protein Kinase calcium calmodulin cyclic AMP egtazic acid fungal protein protein kinase protein kinase (calcium,calmodulin) article autophosphorylation neurospora crassa nonhuman priority journal enzymology gel chromatography ion exchange chromatography isolation and purification Neurospora crassa phosphorylation polyacrylamide gel electrophoresis protein processing Ascomycota Neurospora Neurospora crassa Ca(2+)-Calmodulin Dependent Protein Kinase Calcium Calmodulin Chromatography, DEAE-Cellulose Chromatography, Gel Cyclic AMP Egtazic Acid Electrophoresis, Polyacrylamide Gel Fungal Proteins Neurospora crassa Phosphorylation Protein Kinases Protein Processing, Post-Translational Support, Non-U.S. Gov't |
| description |
DEAE-cellulose column chromatography of Neurospora crassa soluble mycelial extracts leads to the resolution of three major protein kinase activity peaks designated PKI, PKII, and PKIII. PKII activity is stimulated by Ca2+ and Neurospora or brain calmodulin. Maximal stimulation was observed at 2 μM-free Ca2+ and 1 μg/ml of the modulator. The stimulatory effect of the Ca2+-calmodulin complex was blocked by EGTA and by some calmodulin antagonists such as phenothiazine drugs or compound 48/80. PKII phosphorylates different proteins, among which histone II-A at a low concentration and CDPKS, the synthetic peptide specific for Ca2+-calmodulin dependent protein kinases, are the best substrates. Some phosphorylation can be detected in the absence of any exogenous acceptor. PKII activity assayed in the presence of histone II-A or in the absence of exogenous phosphate acceptor (autophosphorylation) co-elute in a DEAE-cellulose column at 0.28 M NaCl. As result of the autophosphorylation reaction of the purified enzyme a main phosphorylated component of 70 kDa was resolved by SDS-polyacrylamide gel electrophoresis. It is possible that this component is an active part of this enzyme. © 1991 Kluwer Academic Publishers. |
| author |
Torres, Héctor Norberto Ochatt, Claudia Margarita |
| author_facet |
Torres, Héctor Norberto Ochatt, Claudia Margarita |
| author_sort |
Torres, Héctor Norberto |
| title |
Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa |
| title_short |
Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa |
| title_full |
Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa |
| title_fullStr |
Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa |
| title_full_unstemmed |
Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa |
| title_sort |
ca2+ calmodulin-dependent protein kinase activity in the ascomycetes neurospora crassa |
| publishDate |
1991 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v102_n2_p155_Ulloa http://hdl.handle.net/20.500.12110/paper_03008177_v102_n2_p155_Ulloa |
| work_keys_str_mv |
AT torreshectornorberto ca2calmodulindependentproteinkinaseactivityintheascomycetesneurosporacrassa AT ochattclaudiamargarita ca2calmodulindependentproteinkinaseactivityintheascomycetesneurosporacrassa |
| _version_ |
1768546066439864320 |