Effect of parathion, malathion, endosulfan and chlordane on porphyrin accumulation and alasynthetase in chick embryo liver
In the present study we investigated the porphyrinogenic ability of 4 pesticides: chlordane, endosulfan, parathion and malathion, all of which are widely used in agriculture. In order to determine whether they affect the heme biosynthetic pathway we studied, in 17-day old chick embryo liver "in...
Guardado en:
| Autores principales: | , |
|---|---|
| Publicado: |
1982
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0300483X_v25_n4_p323_DelCVila http://hdl.handle.net/20.500.12110/paper_0300483X_v25_n4_p323_DelCVila |
| Aporte de: |
| id |
paper:paper_0300483X_v25_n4_p323_DelCVila |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_0300483X_v25_n4_p323_DelCVila2023-06-08T15:27:17Z Effect of parathion, malathion, endosulfan and chlordane on porphyrin accumulation and alasynthetase in chick embryo liver Vila, María del Carmen San Martín de Viale, Leonor Carmen 5 aminolevulinate synthase chlordane dimethyl sulfoxide endosulfan heme malathion parathion porphyrin animal experiment chicken embryo enzyme induction intoxication liver liver toxicity nonhuman 5-Aminolevulinate Synthetase Animal Chick Embryo Chlordan Dimethyl Sulfoxide Endosulfan Liver Malathion Parathion Pesticides Porphyrins Support, Non-U.S. Gov't In the present study we investigated the porphyrinogenic ability of 4 pesticides: chlordane, endosulfan, parathion and malathion, all of which are widely used in agriculture. In order to determine whether they affect the heme biosynthetic pathway we studied, in 17-day old chick embryo liver "in ovo", their effects on the amount of porphyrins and on the activity of δ-ALA synthetase (ALA-S), the first and rate limiting enzyme of this pathway. All of them induced hepatic porphyrin accumulation to a different extent compared with dimethylsulfoxide (DMSO) controls. Parathion as well as endosulfan promoted remarkable increases, chlordane raised porphyrin level in a lower degree and malathion slightly modified it. However, the accumulation observed with malathion was markedly enhanced if the period of incubation was extended to 48 h and, even more, if a second dose was injected during these 48 h. When ALA-S activity was analyzed in the chick embryos treated with parathion or endosulfan no alteration could be found in spite of producing noticeable accumulation of porphyrins. In contrast, chlordane promoted a statistically significant elevation of ALA-S as well as malathion which produced the highest induction observed. These results show that not only organochlorinated but also organophosphorous pesticides affect heme metabolism and that induction of ALA-S and porphyrin accumulation are not parallel. © 1982. Fil:Del C. Vila, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:San Martín de Viale, L.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1982 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0300483X_v25_n4_p323_DelCVila http://hdl.handle.net/20.500.12110/paper_0300483X_v25_n4_p323_DelCVila |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
5 aminolevulinate synthase chlordane dimethyl sulfoxide endosulfan heme malathion parathion porphyrin animal experiment chicken embryo enzyme induction intoxication liver liver toxicity nonhuman 5-Aminolevulinate Synthetase Animal Chick Embryo Chlordan Dimethyl Sulfoxide Endosulfan Liver Malathion Parathion Pesticides Porphyrins Support, Non-U.S. Gov't |
| spellingShingle |
5 aminolevulinate synthase chlordane dimethyl sulfoxide endosulfan heme malathion parathion porphyrin animal experiment chicken embryo enzyme induction intoxication liver liver toxicity nonhuman 5-Aminolevulinate Synthetase Animal Chick Embryo Chlordan Dimethyl Sulfoxide Endosulfan Liver Malathion Parathion Pesticides Porphyrins Support, Non-U.S. Gov't Vila, María del Carmen San Martín de Viale, Leonor Carmen Effect of parathion, malathion, endosulfan and chlordane on porphyrin accumulation and alasynthetase in chick embryo liver |
| topic_facet |
5 aminolevulinate synthase chlordane dimethyl sulfoxide endosulfan heme malathion parathion porphyrin animal experiment chicken embryo enzyme induction intoxication liver liver toxicity nonhuman 5-Aminolevulinate Synthetase Animal Chick Embryo Chlordan Dimethyl Sulfoxide Endosulfan Liver Malathion Parathion Pesticides Porphyrins Support, Non-U.S. Gov't |
| description |
In the present study we investigated the porphyrinogenic ability of 4 pesticides: chlordane, endosulfan, parathion and malathion, all of which are widely used in agriculture. In order to determine whether they affect the heme biosynthetic pathway we studied, in 17-day old chick embryo liver "in ovo", their effects on the amount of porphyrins and on the activity of δ-ALA synthetase (ALA-S), the first and rate limiting enzyme of this pathway. All of them induced hepatic porphyrin accumulation to a different extent compared with dimethylsulfoxide (DMSO) controls. Parathion as well as endosulfan promoted remarkable increases, chlordane raised porphyrin level in a lower degree and malathion slightly modified it. However, the accumulation observed with malathion was markedly enhanced if the period of incubation was extended to 48 h and, even more, if a second dose was injected during these 48 h. When ALA-S activity was analyzed in the chick embryos treated with parathion or endosulfan no alteration could be found in spite of producing noticeable accumulation of porphyrins. In contrast, chlordane promoted a statistically significant elevation of ALA-S as well as malathion which produced the highest induction observed. These results show that not only organochlorinated but also organophosphorous pesticides affect heme metabolism and that induction of ALA-S and porphyrin accumulation are not parallel. © 1982. |
| author |
Vila, María del Carmen San Martín de Viale, Leonor Carmen |
| author_facet |
Vila, María del Carmen San Martín de Viale, Leonor Carmen |
| author_sort |
Vila, María del Carmen |
| title |
Effect of parathion, malathion, endosulfan and chlordane on porphyrin accumulation and alasynthetase in chick embryo liver |
| title_short |
Effect of parathion, malathion, endosulfan and chlordane on porphyrin accumulation and alasynthetase in chick embryo liver |
| title_full |
Effect of parathion, malathion, endosulfan and chlordane on porphyrin accumulation and alasynthetase in chick embryo liver |
| title_fullStr |
Effect of parathion, malathion, endosulfan and chlordane on porphyrin accumulation and alasynthetase in chick embryo liver |
| title_full_unstemmed |
Effect of parathion, malathion, endosulfan and chlordane on porphyrin accumulation and alasynthetase in chick embryo liver |
| title_sort |
effect of parathion, malathion, endosulfan and chlordane on porphyrin accumulation and alasynthetase in chick embryo liver |
| publishDate |
1982 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0300483X_v25_n4_p323_DelCVila http://hdl.handle.net/20.500.12110/paper_0300483X_v25_n4_p323_DelCVila |
| work_keys_str_mv |
AT vilamariadelcarmen effectofparathionmalathionendosulfanandchlordaneonporphyrinaccumulationandalasynthetaseinchickembryoliver AT sanmartindevialeleonorcarmen effectofparathionmalathionendosulfanandchlordaneonporphyrinaccumulationandalasynthetaseinchickembryoliver |
| _version_ |
1768541654756622336 |