Photochromic fluorescence resonance energy transfer (pcFRET): Formalism, implementation, and perspectives

Photochromic FRET (pcFRET), a member of the family of acceptor depletion FRET techniques (adFRET), embodies a general conceptual and experimental scheme based on a coupled system of a fluorescent donor and a photochromic acceptor. The procedure involves the reversible and cyclic spectroscopic deplet...

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Autores principales: Jares, Elizabeth Andrea, Giordano, Luciana, Spagnuolo, Carla Cecilia
Publicado: 2004
Materias:
adFRET
Chemical equilibria
Diheteroarylethene
Dithienylethene
FRET
pcRelKin
Quantum Dot
Relaxation kinetics
Energy transfer
Fluorescence
Phase equilibria
Quantum theory
Rate constants
Resonance
Spectroscopy
Dipoles
Pixels
Photochromism
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0277786X_v5323_n_p13_JaresErijman
http://hdl.handle.net/20.500.12110/paper_0277786X_v5323_n_p13_JaresErijman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_0277786X_v5323_n_p13_JaresErijman
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spelling paper:paper_0277786X_v5323_n_p13_JaresErijman2023-06-08T15:26:20Z Photochromic fluorescence resonance energy transfer (pcFRET): Formalism, implementation, and perspectives Jares, Elizabeth Andrea Giordano, Luciana Spagnuolo, Carla Cecilia adFRET Chemical equilibria Diheteroarylethene Dithienylethene FRET pcRelKin Quantum Dot Relaxation kinetics Energy transfer Fluorescence Phase equilibria Quantum theory Rate constants Resonance Spectroscopy Chemical equilibria Dipoles Pixels Relaxation kinetics Photochromism Photochromic FRET (pcFRET), a member of the family of acceptor depletion FRET techniques (adFRET), embodies a general conceptual and experimental scheme based on a coupled system of a fluorescent donor and a photochromic acceptor. The procedure involves the reversible and cyclic spectroscopic depletion of the acceptor, and was initially conceived for the determination of FRET efficiency on a continuous, pixel-by-pixel basis in the microscopy of living cells. However, the modulation of donor fluorescence in pcFRET has implications for a wide range of applications. We present the formalism for quantitative interpretations of photostationary and kinetic data, from which the relevant kinetic rate constants and quantum yields for the cyclization and cycloreversion reactions of the photochromic acceptor can be derived. The scheme was applied to a model system consisting of a fluorescent donor (Lucifer Yellow) covalently bound to a diheteroarylethene acceptor. In a Perspectives section, we discuss photochromic probes, instrumentation issues, and the potential of pcFRET for analyzing chemical equilibria and kinetics, in the latter case with a new technique we have denoted Photochromic Relaxation Kinetics (pcRelKin). Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giordano, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Spagnuolo, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0277786X_v5323_n_p13_JaresErijman http://hdl.handle.net/20.500.12110/paper_0277786X_v5323_n_p13_JaresErijman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic adFRET
Chemical equilibria
Diheteroarylethene
Dithienylethene
FRET
pcRelKin
Quantum Dot
Relaxation kinetics
Energy transfer
Fluorescence
Phase equilibria
Quantum theory
Rate constants
Resonance
Spectroscopy
Chemical equilibria
Dipoles
Pixels
Relaxation kinetics
Photochromism
spellingShingle adFRET
Chemical equilibria
Diheteroarylethene
Dithienylethene
FRET
pcRelKin
Quantum Dot
Relaxation kinetics
Energy transfer
Fluorescence
Phase equilibria
Quantum theory
Rate constants
Resonance
Spectroscopy
Chemical equilibria
Dipoles
Pixels
Relaxation kinetics
Photochromism
Jares, Elizabeth Andrea
Giordano, Luciana
Spagnuolo, Carla Cecilia
Photochromic fluorescence resonance energy transfer (pcFRET): Formalism, implementation, and perspectives
topic_facet adFRET
Chemical equilibria
Diheteroarylethene
Dithienylethene
FRET
pcRelKin
Quantum Dot
Relaxation kinetics
Energy transfer
Fluorescence
Phase equilibria
Quantum theory
Rate constants
Resonance
Spectroscopy
Chemical equilibria
Dipoles
Pixels
Relaxation kinetics
Photochromism
description Photochromic FRET (pcFRET), a member of the family of acceptor depletion FRET techniques (adFRET), embodies a general conceptual and experimental scheme based on a coupled system of a fluorescent donor and a photochromic acceptor. The procedure involves the reversible and cyclic spectroscopic depletion of the acceptor, and was initially conceived for the determination of FRET efficiency on a continuous, pixel-by-pixel basis in the microscopy of living cells. However, the modulation of donor fluorescence in pcFRET has implications for a wide range of applications. We present the formalism for quantitative interpretations of photostationary and kinetic data, from which the relevant kinetic rate constants and quantum yields for the cyclization and cycloreversion reactions of the photochromic acceptor can be derived. The scheme was applied to a model system consisting of a fluorescent donor (Lucifer Yellow) covalently bound to a diheteroarylethene acceptor. In a Perspectives section, we discuss photochromic probes, instrumentation issues, and the potential of pcFRET for analyzing chemical equilibria and kinetics, in the latter case with a new technique we have denoted Photochromic Relaxation Kinetics (pcRelKin).
author Jares, Elizabeth Andrea
Giordano, Luciana
Spagnuolo, Carla Cecilia
author_facet Jares, Elizabeth Andrea
Giordano, Luciana
Spagnuolo, Carla Cecilia
author_sort Jares, Elizabeth Andrea
title Photochromic fluorescence resonance energy transfer (pcFRET): Formalism, implementation, and perspectives
title_short Photochromic fluorescence resonance energy transfer (pcFRET): Formalism, implementation, and perspectives
title_full Photochromic fluorescence resonance energy transfer (pcFRET): Formalism, implementation, and perspectives
title_fullStr Photochromic fluorescence resonance energy transfer (pcFRET): Formalism, implementation, and perspectives
title_full_unstemmed Photochromic fluorescence resonance energy transfer (pcFRET): Formalism, implementation, and perspectives
title_sort photochromic fluorescence resonance energy transfer (pcfret): formalism, implementation, and perspectives
publishDate 2004
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0277786X_v5323_n_p13_JaresErijman
http://hdl.handle.net/20.500.12110/paper_0277786X_v5323_n_p13_JaresErijman
work_keys_str_mv AT jareselizabethandrea photochromicfluorescenceresonanceenergytransferpcfretformalismimplementationandperspectives
AT giordanoluciana photochromicfluorescenceresonanceenergytransferpcfretformalismimplementationandperspectives
AT spagnuolocarlacecilia photochromicfluorescenceresonanceenergytransferpcfretformalismimplementationandperspectives
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